Research ArticleOpen accessIn the author namesEffects of Wheat Bran, EM Bacteria, Ammonium Nitrate and Fungal Additives on Fermentation and Forage Quality of Rice Straw SilageBin Yang, Jiahuan Liu, Dongmei Ma et al.The global ruminant industry is constrained by a persistent shortage of high-quality roughage, thus rendering the bioconversion of agricultural residues, such as rice straw, a critical priority. Nevertheless, the high lignification, silicification, and nitrogen deficiency of rice straw pose significant biological barriers to traditional ensiling. This study investigated the impact of various additives on rice straw silage quality. Six treatments … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 30 Sep 2026 · 642 accessesView PDFCite
Research ArticleOpen accessIn the author namesSynergistic Effects of Wheat Bran and Inoculants on Fermentation and Nutritional Quality of Soybean Straw SilageLie Yang, Rundong Qin, Jiahuan Liu et al.The efficient valorisation of lignocellulosic biomass from soybean straw into high-quality silage remains a pressing challenge for sustainable livestock production. This study employed a 3×3 factorial design to systematically investigate the interactive effects between wheat bran additions (0%, 10%, 20% of fresh weight) and microbial inoculant regimes (control, Effective Microorganisms, EM+Trichoderma longibrachiatum) on soybean straw silage quality. Two-way ANOVA revealed … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 2 · 1 Jul 2026 · 1,109 accessesView PDFCite
Research ArticleOpen accessIn the author namesStudy on the Polyploidy Induction of Herbicides on Hengshan Astragalus MembranaceusJianxia Liu, Yanmao Gao, Lizhen Liu et al.Studying medicinal polyploid plants is of great significance for the cultivation of traditional Chinese medicine varieties and the extraction of active ingredients. The polyploidization of A. membranaceus (Astragalus membranaceus) was induced using two treatment methods: oryzalin and pendimethalin as mutagens. The results showed that in the same method, the induction effect of polyploidy was affected by the concentration of reagents … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 1 · 14 Apr 2026 · 1,535 accessesView PDFCite
Research ArticleOpen accessIn the author namesUltrasonic Microwave Assisted Extraction, Chemical Characterization and Antioxidant Activity of Polysaccharides from Fig LeavesBaikangzhe Zheng, Yang Miao, Yi Zhou et al.This study employed ultrasonic-microwave-assisted extraction (UMAE) to isolate water-soluble polysaccharides from fig (Ficus carica L.) leaves (FLPs). Polysaccharide yield was used as the primary index to evaluate extraction efficiency. The effects of material-liquid ratio, microwave power, extraction temperature, and extraction time on polysaccharide yield were systematically investigated through single-factor experiments, followed by response surface methodology optimization. Structural characterization and antioxidant … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 3 Mar 2026 · 1,671 accessesView PDFCite
Research ArticleOpen accessIn the author namesExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessIn the author namesStudy on the Enhancement of Hypoglycemic and Antioxidant Activities of Compound Herbal Tea through Solid-State FermentationQing Liu, Xiaowen Que, Yueping Yang et al.This study utilized Aspergillus niger KY2295 for the Solid-State Fermentation (SSF) of a compound tea made from Cinnamomum cassia bark, Glycyrrhiza glabra, Morus abla L. leaf, and Crataegus oxyacantha L. to enhance its hypoglycemic effects. The fermentation process was optimized using single-factor experiments and a Central Composite Design (CCD), with α-amylase and α-glucosidase inhibitory activities as evaluation indices. Following fermentation, … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 20 May 2025 · 5,473 accessesView PDFCite
Research ArticleOpen accessIn the author namesPreparation and Stability of Moisturizing Essence Liquid from Trollius Chinensis Bunge Stem ExtractXiyue Yu, Jiaqing Wu, Wenqiu Zhu et al.The stem, a byproduct of traditional Chinese herbal medicine Trollius chinensis Bunge, has not been effectively developed and utilized for a long time. In this study, the polysaccharides and flavonoids from Trollius chinensis Bunge was used as main ingredient in moisturizing essence liquid, and the preparation and stability of moisturizing essence liquid was studied. The approach was as follows: The … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 27 Mar 2025 · 5,253 accessesView PDFCite
Research ArticleOpen accessIn the author namesDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDFCite
Research ArticleOpen accessIn the author namesExtraction Process Optimization and Functional Characteristics of by Papain Solubilized Collagen from Large Yellow Croaker (Pseudosciaena crocea) SkinBiaoshi Wang, Wenfeng Li, Shumin Liu et al.Through collagen extraction from large yellow croaker (Pseudosciaena crocea) skin using an enzymatic method, the extraction optimization and characterization of collagen from raw materials after papain hydrolysis pretreatment are studied. The extraction process was optimized using the response surface methodology in this study. The findings show that the optimal conditions are a ratio (solvent to solid) of 25.0 mL/g, a … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 4 · 26 Dec 2023 · 5,553 accessesView PDFCite