Author

Qing Liu

Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, China

10 articles in our journals

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Research ArticleOpen accessExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDF Cite

Research ArticleOpen accessStudy on the Enhancement of Hypoglycemic and Antioxidant Activities of Compound Herbal Tea through Solid-State FermentationQing Liu, Xiaowen Que, Yueping Yang et al.This study utilized Aspergillus niger KY2295 for the Solid-State Fermentation (SSF) of a compound tea made from Cinnamomum cassia bark, Glycyrrhiza glabra, Morus abla L. leaf, and Crataegus oxyacantha L. to enhance its hypoglycemic effects. The fermentation process was optimized using single-factor experiments and a Central Composite Design (CCD), with α-amylase and α-glucosidase inhibitory activities as evaluation indices. Following fermentation, … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 20 May 2025 · 5,473 accessesView PDF Cite

Research ArticleOpen accessDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDF Cite

Research ArticleOpen accessEffect of Adding Cellulose on Lipid Accumulation in Cellobiohydrolase and Delta-6 Desaturase Engineered Mucor circinelloides StrainsYao Zhang, Yanxia Wang et al.Despite microbial production of multifunctional gamma-linolenic acid (GLA) being an important and promising way, the high cost is still a major factor limiting industrial applications. Previously, we proposed a novel approach to promote the production of GLA from cellulose, a widely available and cheapest resource, by overexpression of the cellobiohydrolase (CBH2) and delta-6 desaturase (D6) in Mucor circinelloides. In the … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 13 Oct 2023 · 4,731 accessesView PDF Cite

Research ArticleOpen accessPreparation of Rose Vinegar Rich in Gamma-Linolenic Acid and Analysis of its Nutritional Composition and Antioxidant ActivityYao Zhang, Qing Liu et al.Despite the rapid development of various types of fruit vinegar or nutrient-fortified vinegar, the weakened functions or high cost is still the main limiting factor for healthy vinegar. In the present study, a new type of rose vinegar was obtained from raw grain supplemented with natural extract of roses through the cooperative fermentation of multiple microorganisms by adding Mucor circinelloides … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 8 Aug 2023 · 6,388 accessesView PDF Cite

Research ArticleOpen accessPhytochemical Profiles and Anti-Diabetic Potential of the Extract of Pueraria lobata Compound Teabags: Preparation and Optimization Infusion ConditionsQing Liu, Wenyue Shi, Futing Xue et al.A Pueraria lobata root compound teabag using Pueraria lobate root, Stigma maydis, and Prunus mume as the main material was prepared in this study, and the brewing technology was optimized using Response Surface Methodology (RSM). The sensory evaluation, total polysaccharide, and phenolic compound content were used as evaluation indicators and the effect of the compound ratio of raw material was … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 26 Nov 2022 · 5,641 accessesView PDF Cite

Research ArticleOpen accessEvaluation of Antioxidant and Antibacterial Activities of the Polysaccharides from Mycelium and the Culture Medium of Pleurotus Eryngii Strain UN-1Qing Liu, Haodong Wu, Ying Wu et al.Pleurotus eryngii was one of the most precious and ordinary edible mushrooms, and its polysaccharides were the major active substances. In this study, P. eryngii strain UN-1 was cultured in a liquid medium at 25℃ under a shaking of 80 rpm for 7 days. Two crude polysaccharides named CPN and CPNM were obtained from the mycelium of P. eryngii UN-1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 19 Jul 2022 · 6,176 accessesView PDF Cite

Research ArticleOpen accessInhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 CellsShuqing Yang, Zhike Xie, Shuyan Yu et al.In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 26 Apr 2020 · 7,057 accessesView PDF Cite

Research ArticleOpen accessTotal Phenolic, Resveratrol Content, Antioxidant and Antimicrobial Activity in Five Cultivars of Peanut SproutsQing Liu, Shaoqi Li, Yao Zhang et al.Peanut sprout is one of the potential natural sources of bioactive substances. In this study, five different peanut cultivars were conducted to germination, the change in total phenolic compounds, resveratrol content, antioxidant and antimicrobial activity were investigated. The sprouts of five peanut cultivars (Fuhua, Silihong, Dabaisha, Xiaobaisha and Huayu) were extracted using 75% ethanol for 3 h and the crude … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 8,546 accessesView PDF Cite

Research ArticleOpen accessImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDF Cite