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40 results for “human serum”
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Research ArticleOpen accessIn the keywordsThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Metoprolol and Felodipine in Binary Mixture Using Chemometric-Assisted Spectrophotometric and High-Performance Liquid Chromatographic-UV MethodsHesham Salem, Ola M. AbdallahFour methods were developed for simultaneous determination of metoprolol and felodipine without previous separation. In the first method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 222 and 235 nm for metoprolol and felodipine, respectively. The second method depends on first derivative of the ratios spectra by measurements of the amplitudes at 250.1 nm … …American Journal of Applied Sciences, Vol. 4, Issue 9 · 30 Sep 2007 · 8,025 accessesView PDFCite
Research ArticleOpen accessIn the keywordsDetermination of Diloxanide Furoate and Metronidazole in Binary Mixture Using First Derivative of the Ratio-Spectra and High-Performance Liquid Chromatography-UV MethodsNora H. Al-ShaalanTwo methods are presented for simultaneous determination of diloxanide furoate and metronidazole. The first method depends on first derivative of the ratio-spectra by measurements of the amplitudes at 242.5 and 285.5 nm for diloxanide furoate and 225.5 and 300 nm for metrinidazole. Calibration graphs were established for 2-100 μg mL-1 for diloxanide furoate and 1-50 μg mL-1 for metronidazole. In … …American Journal of Applied Sciences, Vol. 4, Issue 2 · 28 Feb 2007 · 7,713 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDFCite
Review ArticleOpen accessIn the abstractThe Impact of Di-2-Ethylhexyl Phthalate (DEHP) in Focus on the Reproductive System and Toxicity in Rat or Mammalian ModelVenkataramanaiah Poli, Srinivasulu Reddy MotireddyAbsalan, F., Saremy, S., Mansouri, E., Moghadam, M. T., Moghadam, A. R. E., & Razie, G. (2016). Effects of Mono-(2-Ethylhexyl) Phthalate and Di-(2-Ethylhexyl) Phthalate Administrations on Oocyte Meiotic Maturation, Apoptosis and Gene Quantification in Mouse Model. Cell Journal, 18(4), 503–513. https://doi.org/10.22074/cellj.2016.4717 Acevedo‐Rodriguez, A., Kauffman, A. S., Cherrington, B. D., Borges, C. S., Roepke, T. A., & Laconi, M. (2018). Emerging … …American Journal of Pharmacology and Toxicology, Vol. 19, Issue 1 · 6 Jun 2024 · 12,990 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Association of Blood Serum Atherogenicity with Risk Factors for Cardiovascular DiseaseTatiana Vladimirovna Kirichenko, Igor Alexandrovich Sobenin et al.Cardiovascular Diseases (CVD) caused by atherosclerosis are the most frequent cause of mortality worldwide, so the identification of new biomarkers of atherosclerosis development is quite actual. The atherogenicity of blood serum is considered as the potential of human serum to cause cholesterol accumulation in primary culture macrophages. The current study aimed to evaluate the association of the atherogenicity of blood … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 30 Oct 2023 · 4,917 accessesView PDFCite
Case ReportOpen accessIn the abstractAn Unusual Case of Pyrexia with BlindnessNaveen, Bharat Bhushan Sharma, Annu Kumari Saw, Akash ParuthiPyrexia is a common clinical problem; causes may vary depending on the clinico- epidemiological settings. Infectious diseases form the most common cause of fever especially in the immunocompromised category. The cause may not be obvious from the initial presentation and a stepwise workup is usually required. Potential diagnostic clues are very important in this regard. Our patient had a fever … …American Journal of Infectious Diseases, Vol. 20, Issue 3 · 2 Dec 2024 · 4,932 accessesView PDFCite
Case ReportOpen accessIn the abstractConsumption of an Extremely Biodiverse Probiotic and a Supplement based on Microbial Chondroitin Sulfate is Associated with Very Low Serum Alpha-N-acetylgalactosaminidase (Nagalase) Activity and Decrease of C-reactive Protein ValuesMichael Carter, Stefania Pacini, Marco RuggieroWe describe the case of a 57-years-old male healthy professional who added to his nutritional plan a novel, extremely biodiverse, probiotic and a supplement based on microbial chondroitin sulfate, vitamin D3 and ultrapure phosphatidylcholine to improve his healthy life expectancy. After three months of such a nutritional regimen, serum alpha-N-acetylgalactosaminidase (nagalase) activity was 0.40 that is a value below the … …American Journal of Immunology, Vol. 16, Issue 1 · 22 Apr 2020 · 13,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractOccurrence of Avian Influenza H5N1 among Chicken, Duck Farms and Human in EgyptMohamed S. Diab, Mohamed S. Abd El Hafez, Mohamed A. Ashry, Wael K. ElfeilAvian influenza (AI) H5N1 virus consider a potential threat to the poultry industry with sever zoonotic effect associated with a high risk on human being associated with the poultry production. The disease become endemic in Egypt and cause more than 359 confirmed human infection cases in the last few years. Samples (serum and tissues) collected from 160 poultry farms that … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 1 · 22 Feb 2019 · 7,643 accessesView PDFCite
Research ArticleOpen accessIn the abstractDoes Iron Modulate Cytokine Production by Desferrioxamine Treated Mononuclear Cells?Hanna Bessler, Meir DjaldettiIron is an essential element for maintaining the normal function of cellular and multicellular organisms and it is closely associated with inflammation and immunity. Macrophages and lymphocytes play an important role in its homeostasis. Hereby we have evaluated cytokine production by iron depleted and iron supplemented human peripheral blood mononuclear cells (PBMC) from healthy adult donors. Non-stimulated or stimulated PBMC … …OnLine Journal of Biological Sciences, Vol. 18, Issue 1 · 7 Jan 2018 · 6,845 accessesView PDFCite