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41 results for “enzyme yield”
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Research ArticleOpen accessIn the keywordsEnhanced Production of Extracellular Alkaline Lipase by an Improved Strain of Pseudomonas aeruginosa MTCC 10,055Deepali Bisht, Santosh Kumar Yadav, Nandan Singh DarmwalProblem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation … …American Journal of Applied Sciences, Vol. 9, Issue 2 · 24 Nov 2011 · 8,760 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Different Functional Microbial Agents on the Biological Characteristics and Rhizosphere Enzyme Activity of Hydroponic CucumberCe Wang, Ouya Zhao, Shiyou Sun et al.This study aims to comprehensively explore the effect of functional microbial agents on healthy soil cultivation and attempt to increase cucumber yield. Three different microbial agents were used for cucumber seedlings by virtue of the hydroponic cultivation method in order to study their effects on enzyme activity and seedling growth and reveal the relationship between rhizosphere enzyme activity and carbon, … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 24 Mar 2025 · 5,510 accessesView PDFCite
Research ArticleOpen accessIn the abstractCyclodextrin Glucanotransferase Activity of Microbial Strains Isolated from Yam-Cultivation Soils in Northwestern BeninCyrille Alode Vodounon, Ténor Dias-Mendel Allode et al.β-Cyclodextrin, an industrially important oligosaccharide, is produced by microbial enzymes such as cyclodextrin glucanotransferase (CGTase). This study investigated ways to optimize the enzymatic activity of CGTase from Bacillus cereus, Bacillus pumilus, Bacillus brevis, and Bacillus subtilis strains isolated from soils in north-western Benin. Cyclization activity of CGTase and the influence of pH, temperature, and metal ions were assessed using conventional … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 21 Sep 2026 · 652 accessesView PDFCite
Research ArticleOpen accessIn the abstractDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDFCite
Research ArticleOpen accessIn the abstractLarvicidal Activity of Spondias purpurea (Sineguelas) Leaf and Hylocereus costaricensis (Dragon Fruit) Stem Extracts Against Aedes aegyptiAlfredo V. Corpuz, Nigell Mikhail A. Gallardo, Ace Danielle C. AveroThe study determined the larvicidal activity of the different concentrations of Spondias purpurea and Hylocereus costaricensis extracts by measuring percent mortality in Aedes aegypti larvae, a primary vector of dengue and other arboviral diseases. The hypothesis proposed that combining both extracts would yield greater larvicidal activity due to potential synergistic interactions. Ethanolic extraction was used to isolate a wide array … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 20 Nov 2025 · 2,417 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Influence of Trichoderma viride Cellulase Enzyme Concentration on Saccharification of Waste Paper MaterialsKhomotso M. P. Mokatse, M. Alfred Mogale, J. Pieter H. Van WykEnzymatic hydrolysis of lignocellulosic materials is one of the major steps in the commercialization process of converting cellulosic substrates into bio-products. The saccharification of lignocellulosic materials is usually achieved by a synergistic action of an enzyme mixture consisting of multiple cellulase enzymes such as endo-and exo-glucanase, cellobiohydrolase and β-glucosidase with different mode of actions. During the enzymatic hydrolysis of the … …OnLine Journal of Biological Sciences, Vol. 21, Issue 4 · 28 Oct 2021 · 6,456 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of Constituents and Bacteriostatic Activity of Essential Oil Extracted from Sea Buckthorn Seeds by Different MethodsHepeng Zhao, Fuyin Zhao, Qian Xiao et al.Sea buckthorn is a homologous plant listed in Chinese pharmacopoeia. The Essential Oils (EOs) of Sea Buckthorn Seeds (SBTS) are widely used in medicine and health products in China, Russia and other countries. The extraction methods of SBTS with advantages of low cost, high yield and high activity have attracted considerable attention. In this study, the extraction rate, chemical compositions … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 4 Jun 2021 · 7,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Xylitol from Agricultural Waste by Enzymatic MethodsMarie K. Walsh, Hussein Fadhil Khliaf, Khalida A. ShakirEnzyme catalyzed production of xylose from autoclaved and non-autoclaved wheat straw was conducted followed by bioconversion of xylose to xylitol using a Crude preparation of Xylose Reductase (CXR) from Candida guillermondii. Ground wheat straw (autoclaved and non autoclaved) was treated at 40 and 50°C for up to 9 h at pH 4.5 with various concentrations of xylanase (12.4 to 37.2 … …American Journal of Agricultural and Biological Sciences, Vol. 13, Issue 1 · 26 Mar 2018 · 9,655 accessesView PDFCite