Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme
Rafika Mila Amanah1, Dita Prameswari Trenggono Putri1, Mohammad Zainal Abidin1, Yuny Erwanto2 & Yuny Erwanto3 Show affiliations
- Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No. 3, Bulaksumur, Yogyakarta, Indonesia
- Department of Animal Products Technology, Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No. 3, Bulaksumur, Yogyakarta, India
- Halal Materials Development, Institute for Halal Industry & System, Gadjah Mada University, LPPT Building, Jl. Kaliurang Km 4.5, Yogyakarta, Indonesia
American Journal of Animal and Veterinary Sciences, Volume 20, Issue 2, pp. 97–102 · doi.org/10.3844/ajavsp.2025.97.102
Abstract
The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis (SDS-PAGE). The results showed the highestcollagen yield was 7.77% from D treatment; the viscosity ranges were 2.18-1.99cP, and the pH ranges were 4.89-4.68. FTIR spectra showed amide A, B, I, II,and III. SDS-PAGE results showed three distinct main bands, consisting of α1,α2, and β chains. In conclusion, collagen could be extracted from Local duckfeet and categorized as collagen type I.
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Amanah, R. M., Putri, D. P. T., Abidin, M. Z. & Erwanto, Y. (2025). Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme. American Journal of Animal and Veterinary Sciences, 20(2), 97–102. https://doi.org/10.3844/ajavsp.2025.97.102
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