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12 results for “batch culture”
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Research ArticleOpen accessPhrase in the titleStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOptimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-CultureArundhati Pal, Amrita Biswas, Sriya Chatterjee, A. K. PaulA moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete … …Current Research in Microbiology, Vol. 6, Issue 2 · 27 Jul 2015 · 7,932 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Nitrogen on Growth and Lipid Content of Chlorella pyrenoidosaSubhasha Nigam, Monika Prakash Rai, Rupali SharmaProblem Statement: In today’s scenario, microalgae are widely recognized as a promising source for biofuel production as a renewable source of energy. They are exceedingly rich in oil, which can be converted to biofuel. This paper deals with one of the method to enhance the lipid content in microalgae. Approach: Microalga, Chlorella pyrenoidosa, was grown autotrophically in batch culture and … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 12,285 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Bacillus Subtilis as Biological Fertilizer by Biotransformation of Corn Steep LiquorXiaojie Ren, Baoyue Liu et al.Corn steep liquor is a kind of intractable organic waste liquidproduced during the processing of corn starch. But it is rich in nutrients,soluble protein, and amino acids and it could be used as a nitrogen source inthe fermentation industry. Many starch processing enterprises cannot directlyutilize corn steep liquor and choose to discharge it directly, thus causingenvironmental pollution. Studies have shown … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 10 Nov 2022 · 7,382 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn vitro Rumen Fermentability of the Pelleted Feed Containing Water Spinach (Ipomoea aquatica)Himmatul Hasanah, Eko Pangestu, Ali Agus, Joelal AchmadiAn in vitro technique based on two stages of batch culture was used to clarify fermentability and digestibility of pellet feed containing water spinach (Ipomoea aquatica). The portion of Pennisetum purpureum in the pellet feed was substituted with Ipomoea aquatica at respective levels of 0% (T1), 20% (T2), 40% (T3) and 60% (T4). The treatments were allocated in a completely … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 1 · 27 Mar 2020 · 7,559 accessesView PDFCite
Research ArticleOpen accessIn the abstractImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDFCite
Research ArticleOpen accessIn the abstractFUNGAL LEACHING OF IRON ORE: ISOLATION, CHARACTERIZATION AND BIOLEACHING STUDIES OF PENICILLIUM VERRUCULOSUMNeha Chaudhary, Trina Banerjee, Neha Ibrahim, G. SibiPyrometallurgical and hydrometallurgical technologies for recovery of metals from low grade ores require high energy and capital costs. Use of microorganisms in leaching of mineral ores is gaining importance due to the implementation of stricter environmental rules. Microbes convert metal compounds into their water soluble forms and are biocatalysts of leaching processes. This study was performed to isolate and characterize … …Current Research in Microbiology, Vol. 5, Issue 1 · 25 Sep 2014 · 7,999 accessesView PDFCite
Research ArticleOpen accessIn the abstractAdvances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol AdditionAydin Berenjian, Raja Mahanama, Andrea Talbot et al.Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 2 · 22 Jun 2012 · 10,539 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimizing of Trichoderma viride Cultivation in Submerged State FermentationHayyan Ismaeil Al-Taweil, Mohammad Bin Osman, Aidil Abdul Hamid, Wan Mohtar Wan YussofProblems statement: A study in Malaysia had shown a strain of Trichoderma viride was isolated from the soil. Questions were raised whether Trichoderma viride submerged state fermentation affected by parameters and which of them is effective? Approach: To investigate the effects of the submerged state fermentation parameters; concentrations of carbon g L-1; (10, 45 and 80), glucose, nitrogen g L-1; … …American Journal of Applied Sciences, Vol. 6, Issue 7 · 31 Jul 2009 · 10,076 accessesView PDFCite