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Author

Michelle Gouzy-Olmos

Pontificia Universidad Javeriana, Colombia
1 article in our journals

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Research ArticleOpen accessStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,780 accessesView PDFCite