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13 results for “Ion Chromatography”
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Research ArticleOpen accessEvery word in the titleCharacteristics of Lipoprotein Peak x Eluted from a Column with the Eluent of High-magnesium Ion Concentration in Lipoprotein Analysis Using the Cation-exchange ChromatographyYuji Hirowatari, Hiroshi Yoshida, Yutaka Ogura et al.The new lipoprotein analysis method using a cation-exchange chromatography, which contains a sulfopropyl-ligand column and two magnesium ion-containing eluents was previously reported. This method can separate serum lipoproteins on the column gel with a magnesium ion concentration gradient and high-density lipoprotein (HDL), low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and an unspecified lipoprotein peak are eluted in order from the column. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,473 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSimultaneous Detection of Nitrite and Nitrate in Celeries and CarrotsDongwu Liu, Jinye Niu, Ling Kong, Zhiwei ChenIn this study, the anions nitrite ion (NO2-) and nitrate ion (NO3-) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the content of NO2- and NO3- in celeries and carrots. NO2- and NO3- were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column and an Iopac … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 May 2016 · 7,214 accessesView PDFCite
Research ArticleOpen accessIn the abstractPurification, Structural Characterization and Anti-Aging and Stress Activities of Cordyceps Mycelium Polysaccharides from Paecilomyces HepialiXiaokang Hu, Huixiu Ma, Guicai Chen et al.This study identified and characterized the bioactive polysaccharide components from Cordyceps mycelium powder (CMP) derived from Paecilomyces hepiali, evaluating their anti-aging and stress-protective activities. Initial screening using Drosophila melanogaster revealed that crude Cordyceps polysaccharides (CCP) exhibited comparable effects to CMP and Cordyceps aqueous extracts (CAE) on natural lifespan and UV stress resistance, suggesting CCP as the primary bioactive component. CCP … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 16 Feb 2026 · 1,698 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Mercuric Ion on the Oxidant Response and Calcium Ion in the Protoplasts of Arabidopsis ThalianaJun Xing, Rui Wu, Hui Yu et al.As a non-essential element for plant growth and development, the heavy metal mercury (Hg) has significant toxicity to plants. The aim of this study was to investigate the effect of mercuric ion (Hg2+) on the oxidant response and calcium ion (Ca2+) in the protoplasts of Arabidopsis thaliana. The protoplast cells were exposed to 5.0, 10.0, and 20.0 mmol/L Hg2+ for … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 3 · 29 Aug 2023 · 4,961 accessesView PDFCite
Research ArticleOpen accessIn the abstractNovel LC-MS/MS Method for CJ-023423 (Grapiprant) Determination in Rabbit PlasmaElena Baralla, Virginia De Vito, Maria Vittoria Varoni et al.Grapiprant is a new anti-inflammatory drug that preferentially targets the EP4 receptor of prostaglandin E2 limiting the wide range of adverse effects caused by the classical non steroideal anti-inflammatory drugs. The aim of this study was to develop and validate a new, simple, sensitive and rapid Liquid Chromatography tandem Mass Spectrometry method (LC-MS/MS) in order to quantify this novel drug … …American Journal of Animal and Veterinary Sciences, Vol. 13, Issue 2 · 24 May 2018 · 7,988 accessesView PDFCite
Research ArticleOpen accessIn the abstractDirect Analysis of Six Pharmaceuticals using Online Solid Phase Extraction Liquid ChromatographySiti Norbayu Mohd Subari, Rozita Osman, Norashikin SaimIn this study, three parameters (sample volume, cleanup and elution step) in large volume injection online Solid Phase Extraction Liquid Chromatography with diode array detection (SPE-LC) were optimized for the determination of six pharmaceuticals in wastewater samples. Preconcentration and separation were carried out using 2 columns, Dionex Ion Pac AG14A and Acclaim Polar Advantage II. Response Surface Methodology (RSM) was … …American Journal of Applied Sciences, Vol. 14, Issue 5 · 31 Mar 2017 · 7,513 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction, Purification and Characterization of Fish Chymotrypsin: A ReviewLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly et al.Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 10,585 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite
Research ArticleOpen accessIn the abstractOligoglucans as Elicitors of an Enzymatic Antioxidant System in Zucchini Squash (Cucurbita pepo L.) Seedlings at Low TemperatureAbel Ceron-Garcia, Gustavo Adolfo Gonzalez-Aguilar, Irasema Vargas-Arispuro et al.Abstract: Problem statement: Non-controlled low temperatures affect physiological and biochemical processes leading to Reactive Oxygen Species (ROS) accumulation in plants, which normally are stabilized by the antioxidant system. Superoxide Dismutase (SOD), Ascorbate Peroxidase (APX) and Catalase (CAT) are the most important ROS-detoxification enzymes in stressed plants. Approach: The activity of these enzymes were examined in response to fungal Oligoglucans (OG) … …American Journal of Agricultural and Biological Sciences, Vol. 6, Issue 1 · 15 Dec 2010 · 8,306 accessesView PDFCite