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29 results for “α-amylase”
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Research ArticleOpen accessEvery word in the titleThe Deproteinization, Antioxidant Acticities and Inhibitory Effect on α-Amylase of Polysaccharides from Corn SilkYaping Li, Songbiao Shi, Xiudong Yang, Hongli ZhouAn approach of deproteinization of polysaccharides from corn silk (CSPs) was established to improve the purity and activities of CSPs by investigating Sevag method, trichloroacetic acid (TCA) method and HCl method. Deproteinization rate, polysaccharide retention rate, antioxidant activities and inhibition α-amylase effects were used as indicative mark to select suitable deproteinization method of CSPs. The crude CSPs was extracted by … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 23 May 2019 · 9,294 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAmino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)Raksmont Ubonbal, Saijai Posoongnoen, Jureerut Daduang et al.The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 3 · 19 Aug 2015 · 7,215 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIsolation of Amylolytic Bacteria from Viscera of Labeo rohita (Ham.) and Optimization of Alpha Amylase ExtractionDebabrat Baishya, Hridip Kumar SarmaProblem statement: Fish is largely consumed in the north-eastern part of India resulting in the generation of huge amount of fish viscera in the market places. These waste generated is not properly managed for disposal. Under such circumstances it is necessary to properly utilize this waste regenerating the possibility of environment friendly disposal. Besides, industrially and commercially useful biological products … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 8,051 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,329 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCharacteristics of a Novel Highly Thermostable and Extremely Thermophilic Alkalitolerant Amylase from Hyperthermophilic Bacillus Strain HUTBS71Farouk Al-Quadan, Hazem Akel, Rasha NatshiProblem statement: This study reported the purification and characterization of a novel highly thermostable alkaline amylase from a newly isolated Bacillus strain HUTBS71. Approach: The enzyme was purified using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. Results: Maximum amylase activity (72 U mL-1) was obtained at 100°C after 10 min of incubation. The enzyme was purified 24 fold … …OnLine Journal of Biological Sciences, Vol. 9, Issue 3 · 30 Sep 2009 · 7,400 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleStudy on Salivary Glands α-amylase In Wheat Bug Eurygaster maura (Hemiptera: Scutelleridae)Mohammad Mehrabadi, Ali R. Bandaniα-amylase activity in the salivary glands of Eurygaster maura was determined by biochemical experiments. Some of adult insect was collected and their salivary glands isolated and characterized. Enzyme samples from salivary glands of adults were prepared by the method of Cohen with slight modifications. α-Amylase activity was assayed based on Bernfeld method by the dinitrosalicylic acid (DNS) procedure. The activity … …American Journal of Applied Sciences, Vol. 6, Issue 4 · 30 Apr 2009 · 7,643 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAssessing of α-Amylase Activity of Midgut in Wheat Bug Eurygaster mauraMohammad Mehrabadi, Ali R. BandaniTo determine of α-amylase activity in the midgut of Eurygaster maura, some of adult insect was collected and their gut isolated and characterized. Enzyme samples from midguts of adults were prepared by the method of Cohen with slight modifications. α-Amylase activity was assayed based on Bernfeld method by the dinitrosalicylic acid (DNS) procedure. The activity of α-amylase in midgut was … …American Journal of Applied Sciences, Vol. 6, Issue 3 · 31 Mar 2009 · 7,625 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleA Thermostable α-Amylase Producing Natural Variant of Bacillus spp. Isolated From Soil in IranIraj Rasooli, Shakiba D. A. Astaneh, Hojjat Borna, Kamal A. BarchiniThermophilic processes appear more stable, rapid and less expensive and facilitate reactant activity and product recovery. Amylases have a quarter of the world enzyme market and thermostable a-amylases possess extensive commercial applications. Since little work has been done on strain isolation, growth and enzyme yield optimization, the level of thermophilic enzyme production remains relatively low. Therefore, large scale exploitation of … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 3 · 30 Sep 2008 · 8,902 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Amylase Activity by Modulating Abiotic Factors and Enzyme Stability in the Thermophilic Bacterial IsolatesAnthoni Agustien, Denny Bendrianis, Feskaharny Alamsyah et al.Amylase is one of the enzymes that is widely used in bioprocess technology, amylase from different microbial sources also varies in form, so it is suitable for its specific application. The purpose of this study was to determine the optimum temperature, pH, agitation, and stability of the amylase enzyme from two thermophilic bacterial isolates, TUA-07 and TUA-09. This study used … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 7 Feb 2024 · 5,493 accessesView PDFCite
Review ArticleOpen accessIn the keywordsOptimization of Red Sorghum (Sorghum bicolor (L.) Moench) Malt Mashing Using Response Surface MethodologyArthur Kapepa Amisi et al.The Response Surface Methodology (RSM) was used to optimize the Released Glucose Equivalent during red sorghum malt mashing. Three parameters were tested: the β-amylase (EC 3.2.1.2) temperature level (min value = 62°C and max value = 63°C, TB), the α-amylase (EC 3.2.1.1) temperature level (min value = 72.5 and max value = 77.5°C, TA) and the duration of each stage … …American Journal of Applied Sciences, Vol. 21, Issue 1 · 28 Nov 2024 · 8,092 accessesView PDFCite