Author

Dessy Natalia

Institut Teknologi Bandung, Indonesia

4 articles in our journals

Articles where this person is an author, newest first. People are matched by their name as printed (no ORCID iD is recorded for them), not by name alone; search the name as text to find namesakes too.

Research ArticleOpen accessThe Use of α-MF Signal Peptide Without Spacer for Producing Insulin Aspart Precursor in Pichia pastoris KM71Popi Asri Kurniatin, Fifi Fitriyah Masduki, Neni Nurainy et al.A spacer peptide (Glu-Ala repeats) connects N-terminal of a polypeptide precursor to the signal peptide of α-mating factor (α-MF) from Saccharomyces cerevisiae. During the polypeptide precursor secretion, the signal peptide will be first cleaved by signal peptidase in the endoplasmic reticulum, followed by Kex2 endopeptidase and finally the spacer peptide is removed by dipeptidyl aminopeptidase in the Golgi apparatus. In … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 31 Oct 2019 · 9,026 accessesView PDF Cite

Research ArticleOpen accessProduction of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coliDessy Natalia, Christian Heryakusuma, Fernita Puspasari et al.Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 10 Oct 2018 · 10,346 accessesView PDF Cite

Research ArticleOpen accessIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,329 accessesView PDF Cite

Research ArticleOpen accessCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDF Cite