Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)

The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid residues for the active site. It was classified as a member of α-amylase family 13 because it has an active Domain A, similar to a (α/β)₈-barrel structure. Three-dimensional structural predictions revealed that this partial sequence completely covered all of necessary domains for amylase activity.