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172 articles for “Sequences”
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Research ArticleOpen accessIn the abstractSequence Analysis of Scaffold/Matrix Attachment Regions (S/MARs) From Human Embryonic Kidney and Chinese Hamster Ovary CellsNur Shazwani Mohd Pilus, Azrin Ahmad, Nurul Yuziana Mohd YusofBinding of intergenic Scaffold/Matrix Attachment Regions (S/MARs) to nuclear matrix proteins is believed to poise adjacent genes for transcription by forming chromatin loops. Vector constructs containing Scaffold/Matrix Attachment Regions (S/MAR) flanking the gene of interest, therefore, are able to enhance recombinant protein expression in mammalian cells. We compared two methods that are based on buffers containing 2M NaCl and Lithium-3,5-diidosalicylate … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 8 Oct 2018 · 7,637 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Genomics of Xylella fastidiosa Subsp. Pauca and Insights on its Current and Potential Movements in EcuadorCarlos Bolanos-Carriel, Eliana Granja Guerra, Jaris Veneros et al.Xylella fastidiosa subsp. Pauca causes Coffee Leaf Scorch (CLS). It is a major threat to coffee production in most South and Central American countries. X. fastidiosa is prone to intersubspecific recombination, and it is believed that this species became pathogenic in citrus and coffee due to recent mutation events and gene flow between closely related strains. CLS epidemics have not … …OnLine Journal of Biological Sciences, Vol. 25, Issue 1 · 23 Nov 2024 · 6,176 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimizing N-linked Glycosylation Site Prediction in Human Proteins with Ensemble Stacking and Cross-ValidationMubina Malik, Jaimin UndaviaThe most frequent post-translational modification of proteins in all territories is glycosylation which impacts many biological activities. The most significant and critical of these modifications is N-linked glycosylation which is associated with various human diseases including diabetes cancer Inflammation Alzheimers and atherosclerosis. This article illustrates recent advances in knowledge of biology that are eventually targeting the computer science sector. Moreover-identification … …Journal of Computer Science, Vol. 20, Issue 12 · 20 Nov 2024 · 5,005 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Analysis of Bifidobacteriaceae in a Fermented Milk and Colostrum Product in Relation to Immune FunctionMarco RuggieroThis study investigates the microbial composition of a commercially available Swiss fermented milk and colostrum product with a history of use in chronic condition management since 2014. The analysis, performed using the Axiom Microbiome Array, identified a high number of targets corresponding to microbial species classified as probiotics belonging to the Bifidobacteriaceae family. The array provided 28 unique targets, each … …American Journal of Immunology, Vol. 19, Issue 1 · 2 Aug 2024 · 9,054 accessesView PDFCite
Research ArticleOpen accessIn the abstractStress Resistance Physiological Traits in Maize Under High-Concentration Salt StressMingxia WangTo quantitatively analyze the stress-resistant physiological characteristics of maize under high-concentration salt stress, a combination of statistical analysis and quantitative recursive analysis is selected to conduct salt stress experiments on maize seedlings. 20 treatments are set up, with each treatment consisting of 5 pots and 3 plants per pot. The experiment is conducted without the involvement of any animals. Physiological … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 26 Apr 2024 · 4,872 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,937 accessesView PDFCite
Research NotesOpen accessIn the abstractAn Artificial Design Technique to Optimize Signal PeptideGao Cui-Fang, Wang Sen, Tian Feng-Wei et al.To determine optimal artificial signal peptide candidates for the possibility of creating high levels of secretion of heterologous proteins, substitution and redesign of amino acid sequences in the H-domain of the signal peptide was theoretically attempted. The method was based on comprehensive score matrix and Markov transfer matrix, which can make the artificial sequences maintain the structural characteristics and original … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 3 · 21 Jul 2017 · 8,069 accessesView PDFCite
Research ArticleOpen accessIn the abstractMorphological Aspects of the Inferior Frontal Gyrus and its Relations with Brain Hemispheres and Gender: A Study with in vivo Magnetic Resonance ImagingSergio Murilo Georgeto, Munir Antônio Gariba, Luiz Roberto AguiarThis study aims to evaluate the reliability of an anatomy-topographic location method for the anatomical references that form the inferior frontal gyrus and compare the findings of both studied brain hemisphere as well as the genre. Two different pulse sequences-gradient-echo T1 (T1 GRE) and reversal recovery gradient-echo T1 (T1 IR GRE) were used for such purpose. The sample consisted of … …Neuroscience International, Vol. 7, Issue 1 · 22 Mar 2017 · 7,277 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn silico Analysis of 4CL Family in Scutellaria baicalensis through Biocomputational Tools and ServersGuoming Li, Xiaozhong Lan, Xiaorong Shui et al.The gene sequences of 4-coumarate:coenzyme A ligase (4CL) family of Scutellaria baicalensis came from the GenBank Database. With help of some bioinformatics tools such as Vector NTI Suite 8, ProtParam and SWISS-MODEL and so on, a series of biological information of their nucleic acid sequences and amino acid sequences were predicted and analyzed and the results were revealed as following: … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 1 · 15 Feb 2017 · 7,866 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of Antagonistic Activity of Two Trichoderma Species New to Kazakhstan Against Soil PathogensYelena Rakhimova, Assem Assylbek, Lyazzat Kyzmetova et al.The high efficiency of representatives of the Trichoderma genus in suppressing a wide range of plant pathogens contributes to the conduct of large-scale research on the search for local strains. The purpose of this study is to characterize the antagonistic activity of two Trichoderma species new to Kazakhstan (T. pararogersonii and T. rossicum) isolated from the rhizosphere of Picea schrenkiana … …OnLine Journal of Biological Sciences, Vol. 23, Issue 4 · 17 Oct 2023 · 4,461 accessesView PDFCite