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241 results for “In Vitro Study”
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Research ArticleOpen accessIn the abstractInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDFCite
Research ArticleOpen accessIn the abstractPunicalagin Suppresses Mediators Involved in Labor Onset and Progression in vitroCaitlyn Nguyen-Ngo, Jane C Willcox, Martha LappasSpontaneous preterm birth is the leading cause of neonatal morbidity and mortality. Inflammation plays a central role in the activation of myometrial contractions and rupture of fetal membranes associated with spontaneous preterm birth. The pomegranate polyphenol punicalagin is known to possess anti-inflammatory properties. In this study, we aimed to determine the effect of punicalagin on pro-inflammatory and pro-labor mediators in … …American Journal of Immunology, Vol. 17, Issue 1 · 22 Dec 2021 · 7,609 accessesView PDFCite
Research ArticleOpen accessIn the abstractLignin Content, Ligninase Enzyme Activity and in vitro Digestability of Sugarcane Shoots using Pleurotus ostreatus and Aspergillus oryzae at Different Fermentation TimesRoni Pazla, Novirman Jamarun, Lili Warly et al.This study aims to obtain the best lignin content, ligninase enzyme activity and in vitro digestibility value of fermented sugarcane shoots using Pleurotus ostreatus and Aspergillus oryzae at different fermentation times. The research process is divided into 2 stages. Stage 1 treatment samples include A1B1 = Sugarcane shoots fermented with Pleurotus ostreatus for 14 days; A1B2 = Sugarcane shoots fermented … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 30 Sep 2021 · 7,298 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudies on Pharmacokinetics and Liver Microsome Metabolism of Nuciferine in Different Species of AnimalsDong Xiao-Chen, Liu Ming-Qi, Liu Jun et al.This study aimed to compare the characteristics of pharmacokinetics and liver microsome metabolism among different species of animals including rabbit, rat and mouse and healthy New Zealand white rabbits, SPF Sprague Dawley (SD) rats and ICR mice were given intraperitoneal injection of nuciferine at a dose of 50 mg per kg body weight. Blood samples were collected via caudal vein … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 3 · 12 Aug 2021 · 6,468 accessesView PDFCite
Research ArticleOpen accessIn the abstractSupplementation of Prill Fat Derived from Palm Oil on Nutrient Digestibility and Dairy Cow PerformanceLolita Udin Riestanti, Despal, Yuli RetnaniPrill fat is a hydrogenated vegetable oil which contains more than 85% palmitic acid with a high melting point. Due to this reason, it does not melt at rumen temperature and bypass rumen degradation. This study was aimed to determine the optimum level of prill fat (86% palmitic acid) supplementation in dairy ration on fermentation characteristics, feed digestibility, milk production, … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 2 · 1 Jul 2021 · 8,750 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of the Protein Profile of Cassava Plantlets (Manihot esculenta Crantz.) Resistance to Fusarium Wilt DiseaseEndang Nurcahyani, Hardoko Insan Qudus, Ferina EvlinFusarium wilt disease is still a production constraint in Cassava (Manihot esculenta Crantz.) cultivation. The disease is caused by the fungus Fusarium oxysporum (Fo), which to date has not been cured. It is expected that the use of varieties of cassava that are resistant to Fusarium wilt is an important alternative for disease control. Induced Resistance cassava research on Murashige … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 18 Jun 2021 · 6,777 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) GenotypesMd. Nazmul Hasan, Fahmid H. Bhuiyan, Hammadul Hoque et al.Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 2 · 19 Apr 2021 · 7,859 accessesView PDFCite
Research ArticleOpen accessIn the abstractAmphetamine Stimulates Protein Kinase and Calcium Influx to Increase Corticosterone and Aldosterone Secretion from Male Rat Adrenal CellsLing-Ling Chang, Wan-Song Alfred Wun, Cai-Yun Jian, Paulus S. WangAmphetamine is a potent central nervous system stimulant. Clinical trials have demonstrated that in healthy adults, low (therapeutic) doses of amphetamine can improve i.e., cognition, memory, attention behavior. An amphetamine overdose can affect cardiovascular, central nervous system, musculoskeletal, respiratory, urinary, or sexual function. Furthermore, amphetamine can activate the hypothalamic-pituitary-adrenal axis to increase glucocorticoids and mineralocorticoids released from adrenal. The object … …American Journal of Pharmacology and Toxicology, Vol. 16, Issue 1 · 10 Mar 2021 · 7,872 accessesView PDFCite
Research ArticleOpen accessIn the abstractPharmacological Properties of Bioactive Extracts from the Rhizome of Aristolochia longa LAmal El Hamsas El Youbi, Dalila BoustaAristolochia longa is a medicinal plant well known in Moroccan folk medicine for its activity against constipation, intestinal infections and skin diseases. The present study was performed to evaluate in vitro and in vivo immunomodulatory and cytotoxic activities of aqueous and protein extracts of A. longa. The in vitro immunomodulatory results showed that aqueous extract at 0.1 mg/mL enhanced T-lymphocyte … …American Journal of Pharmacology and Toxicology, Vol. 15, Issue 1 · 21 Jul 2020 · 7,456 accessesView PDFCite