Author

Masindi Lottus Mphaphathi

Germplasm Conservation and Reproductive Biotechnologies, Agricultural Research Council, Animal Production, Private Bag X2, Irene, South Africa

9 articles in our journals

Articles where this person is an author, newest first. People are matched by their name as printed (no ORCID iD is recorded for them), not by name alone; search the name as text to find namesakes too.

Research ArticleOpen accessOptimizing Boar Epididymal Sperm Preservation: Comparative Analysis of Slicing Float-Up vs. Flushing and BTS vs. Andromed® Extenders on Sperm Motility and Morphometry TraitsMamonene Angelinah Thema, Ntuthuko Raphael Mkhize, Gerhard van der Horst et al.Epididymal sperm collection represents a valuable strategy for preserving genetics from deceased or castrated boars of superior breeding value. The two primary collection methods-slicing float-up (high sperm yield) and flushing (low blood contamination risk)-differ in sperm recovery efficiency, while semen extenders provide cryoprotective nutrients that protect against cold shock during preservation. This study compared the effects of slicing float-up versus … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 14 Mar 2026 · 1,794 accessesView PDF Cite

Research ArticleOpen accessAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDF Cite

Research ArticleOpen accessSupplementation with Different Concentrations of Dithiothreitol and Glutathione During Cryopreservation of Semen from Large White BoarsMahlatsana Ramaesela Ledwaba, Masindi Lottus Mphaphathi et al.The study aims to compare different concentrations (0, 2.5, 5, 7.5 and 10 mM) of antioxidants, Dithiothreitol (DTT) and Glutathione (GSH), on sperm parameters following the freeze-thawing of semen from large white boars. Three large white boars were selected for semen collection and the collected semen was sent to the lab for analysis. Following dilution with Beltsville thawing solution and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 3 Jun 2024 · 5,517 accessesView PDF Cite

Research ArticleOpen accessEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDF Cite

Research ArticleOpen accessFrozen Semen Quality of Kolbroek Boars Concerning Glutathione ConcentrationsLerato Deirdre Sehlabela, Masindi Lottus Mphaphathi et al.Glutathione (GSH) is an antioxidant that is being studied for its ability to improvecellular responses to semen preservation challenges. The research goal aimed toevaluate the impact of introducing different glutathioneconcentrations to boar semen freezing extender (0, 1, 5, and 10 mM) onthe Kolbroek boar sperm's quality after being frozen and thawed. For 2 h, thecollected sperm-rich fraction was chilled at … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 1 Apr 2023 · 6,005 accessesView PDF Cite

Research ArticleOpen accessComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDF Cite

Research ArticleOpen accessEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDF Cite

Research ArticleOpen accessStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,915 accessesView PDF Cite

Research ArticleOpen accessEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,591 accessesView PDF Cite