53 results for “Enzymatic Activity”

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Research ArticleOpen accessIn the abstractBiological Activity of Lactic Acid Based Feed Additive and its Physiological Effect on the Animal OrganismNadezhda Nikolaevna Lantseva, Alexander Nikolaevich Shvydkov, Lyudmila Anatolyevna Ryabukha, Veronica Valeryevna EvsyukovaThe data obtained as a result of the study of the enzymatic activity of the lactic acid based feed additive LAFA, based on various bacteria, are consistent with the results of the research presented in the publications on the matter, concerning the fact that probiotic bacteria have enzymatic activity. The conducted studies revealed the leading enzymatic properties of bifidobacteria in … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 4 Oct 2018 · 7,416 accessesView PDF Cite

Research ArticleOpen accessIn the abstractStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBrevibacillus Thermoruber 9X-GLC, Bacteria Isolated from Hot Compost, Producer of a Beta-Glucosidase Resistant to Glucose InhibitionAlejandro Sosa-Peinado, María Eugenia Hidalgo-Lara, Miguel Ángel Plascencia-Espinosa et al.The thermotolerant strain Brevibacillus thermoruber 9X-GLC produces large amounts of β-D-glucosidase and cellobiohydrolase when grown on cellobiose and avicel. 9X-GLC was isolated from hot compost of sugarcane bagasse and filter mud and selected for its high enzymatic activity from a Bacilli collection of 77 isolates from soils and compost. Culture supernatants and ultrafiltered concentrates from shaken flask cultures of 9X-GLC … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 20 Oct 2017 · 8,153 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIs RNA Binding in the Rossmann Fold Essential for GAPDH Intranuclear Functions?Natalia Krynetskaia, Manali Phadke, Evgeny KrynetskiyIn addition to its prominent enzymatic activity, GAPDH is an enigmatic component of multiple unrelated biochemical entities. In this study, we explored a series of mutated GAPDH and fusion EGFP-GAPDH polypeptides and compared nuclear accumulation, intranuclear mobility and RNA binding properties of the wild type and variant GAPDH proteins. Our results revealed that RNA binding to T99I-mutated GAPDH with non-functional … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 Jun 2016 · 7,657 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDistribution of Polyphenol Oxidase in some Cruciferae VegetablesAndi Nur Faidah Rahman, Februadi Bastian, Jumriah Langkong, Shuji FujitaEnzymatic oxidation of polyphenolic compounds in damaged tissue of many fruits and vegetables induced undesirable browning as results in decreased marketability of products. A new type of polyphenol oxidase (PPO) that is “Phloroglucinol Oxidase (PhO)” have been reported in turnip and cabbage, and the purified enzymes also had strong peroxidase (POD) activity. Distribution of PPO from different plant source of … …American Journal of Applied Sciences, Vol. 13, Issue 1 · 31 Jan 2016 · 8,093 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAnalysis of Phenolic Content and Antioxidant Properties of Selected Cowpea Varieties Tested in Bovine Peripheral BloodSarah Adjei-Fremah, Louis EN Jackai, Mulumebet WorkuCowpea is an important grain legume with a dual purpose function as a food and feed resource. Cowpea contains phenolic compounds that are beneficial to human and animal health. We evaluated the phenolic content, condensed tannin content and antioxidant capacities of methanol extracts of seed and leaf of seven varieties commonly used in food and animal feed. The total phenolic … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 4 · 19 Dec 2015 · 7,870 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPrenatal Ethanol Exposure Affects the Proliferation and Differentiation of the Osteoblasts from Newborn RatsIsabel Chaves Silva Carvalho, Dennia Perez de Andrade, Noala Vicensoto Moreira Milhan et al.Alcohol exerts teratogenic effects and its consumption during pregnancy may cause various alterations in the fetus, including deficit of bone development. The objective of this study was to evaluate the initial responses, on osteoblasts isolated from newborn rat calvaria, after prenatal ethanol exposure. Nine pregnant rats were divided into three groups according to the diet fed during pregnancy: Rats fed … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 14 Jul 2015 · 6,704 accessesView PDF Cite

Research ArticleOpen accessIn the abstractImpact Assessment of Soil Contamination with Antibiotics (for Example, an Ordinary Chernozem)Yuliya Viktorovna Akimenko, Kamil Shagidullovich Kazeev, Sergey Ilyich KolesnikovThe environmental behavior of antibiotics is not well known and the precise environmental risk assessment is not practical. We studied the impact antibiotics (benzylpenicillin, pharmasin and nystatin) at different concentrations (100 and 600 mg kg-1) on population densities of microorganisms and enzymatic activity of ordinary chernozems in model experiments. The applied doses of antibiotics had definite suppressing effects on population … …American Journal of Applied Sciences, Vol. 12, Issue 2 · 29 Apr 2015 · 7,852 accessesView PDF Cite

Research ArticleOpen accessIn the abstractVARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY LENTINULA EDODES IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSECitlalli Harris-Valle, Elisa Valenzuela-Soto, Alfonso Sánchez et al.The white-rot basidiomycete Lentinula edodes is the second most edible mushroom cultivated on the world. This fungus decomposes cell-wall associated macromolecules, is a natural degrader of lignin polymers. The differences in enzyme activities between strains of L. edodes provided useful information about the participation of enzymes in different development stages of the fungus. The effect of lignin on the fungal … …American Journal of Agricultural and Biological Sciences, Vol. 9, Issue 3 · 28 Apr 2014 · 7,845 accessesView PDF Cite