61 results for “PCR detection”

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Research ArticleOpen accessIn the abstractComparison of Diagnostic Tests for Fosfomycin Resistance Detection in Clinically Significant EnterobacteralesSantoshi Maruti Naik, Biranthabail Dhanashree, Himani KotianMultidrug resistance in Enterobacterales has triggered interest in old antibiotics like fosfomycin. Various methods to determine fosfomycin resistance are time-consuming and yield unpredictable results. Hence, the study was undertaken to compare the rapid fosfomycin NP test with the recommended agar dilution test and few other tests. In this cross-sectional, time-bound, prospective study, Enterobacterales (N = 110) were tested for their … …OnLine Journal of Biological Sciences, Vol. 25, Issue 3 · 21 Nov 2025 · 2,113 accessesView PDF Cite

Research ArticleOpen accessIn the abstractMolecular Identification of Domestic and Wild Boars Using Cytochrome-b Specific Primers for Halal AuthenticationRifqi, Edi Suryanto, Ari Surya Sukarno et al.The subject of adulterating wild boar and pig meat products with other animal meat is delicate, particularly in the Muslim nation of Indonesia. In recent years, there has been a lot of concern over halal meat products that contain wild boar and pig meat due to Economically Motivated Adulteration (EMA). However, there are limited studies of PCR-DNA-based to discriminate between … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 3 · 14 Nov 2025 · 2,378 accessesView PDF Cite

Research ArticleOpen accessIn the abstractImpact of Multiplex PCR Panel on Reducing Broad-Spectrum Antibiotic Use in Ventilator-Associated PneumoniaMaría Inés Staneloni, Emilio F. Huaier Arriazu et al.The extended use of broad-spectrum antimicrobials contribute to the growing threat of antimicrobial resistance. This study evaluated the impact of a multimodal strategy aiming at reducing the duration of broad-spectrum antimicrobial therapy in Ventilator-Associated Pneumonia (VAP). Conducted in a single Intensive Care Unit (ICU), this quasi-experimental, retrospective study compares a pre-intervention period (08/01/2018-07/01/2019) with an intervention period (08/01/2022-07/01/2023). Adult patients … …American Journal of Infectious Diseases, Vol. 21, Issue 1 · 8 Jul 2025 · 6,582 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInvestigating Campylobacter Spp. in Mekong Delta Duck Farms: Prevalence, Risk Factors, and Antibiotic ResistanceNguyen Vu Thuy Hong Loan, Le Trung Kien, Do Ngoc Yen Phuong et al.Campylobacter spp. is a leading cause of gastroenteritis worldwide, contributing to significant health and economic burdens, especially in poor hygiene practices. Investigating this bacterium's prevalence and antibiotic resistance in ducks from the Mekong Delta is essential, as the region's extensive duck farming industry presents a potential risk for transmission to humans through the food chain. During August 2024 to February … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 8 Feb 2026 · 1,615 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPrevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, AlgeriaSabrina Rabehi, Bakir Mamache, Taha Mossadak Hamdi et al.The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 15 Oct 2021 · 6,997 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPCR Diagnosis for the Identification of the Virulent Gene of Salmonella in Poultry MeatD. K. Zhanabayeva, A. Y. Paritova, G. K. Murzakaeva et al.The purpose of this study was to conduct microbiological studies of poultry meat imported into Mongolia and establish the serotypes and plasmids of Salmonella and determine the virulence of the gene encoding antibiotic resistance and its transmigratory properties. The PCR molecular biological method was used and according to the results, Salmonella enteritidis was identified in chicken carcasses of the American … …OnLine Journal of Biological Sciences, Vol. 21, Issue 3 · 27 Aug 2021 · 6,865 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAlcohol Intubation and Synergistic Effect of Folic Acid and Egg Yolk Powder Solution on Alcohol Dehydrogenase [ADH] Class I Gene Expression and Single Nucleotide Polymorphism Frequency in Parent and Prenatal Alcohol Exposed Wistar RatsAdejoke Olukayode Obajuluwa, Oluyinka Ajibola Iyiola, Joseph Akintade Morakinyo et al.Point mutation detections in alcohol metabolizing genes could unravel molecular targets of gestational alcohol and epigenetic remodeling capacity of maternal nutrition, which underscores growth and health endpoints in Prenatal Alcohol Exposed (PAE) fetuses. gene expression was assayed using sequenced target and internal control gene primers in a two-step thermal cycling process from isolated RNA in purely bred alcohol male and … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 22 Nov 2023 · 4,857 accessesView PDF Cite

Research NotesOpen accessIn the abstractEvaluation of A Rapid IgM-IgG Combined Antibody Test for SARS-CoV-2 Infection: Single Italian Center StudyKatia Margiotti, Marina Cupellaro, Sabrina Emili et al.The need for timely establishment of diagnostic assays of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is demanded in laboratories worldwide. We evaluated the performance of a flow immunoassay which can detect IgM an IgG antibodies simultaneously against SARS-CoV-2 virus in human blood within 15 min. Among the 132 positive novel Coronavirus Disease 19 (COVID-19) cases, 126 tests were consistent … …American Journal of Infectious Diseases, Vol. 16, Issue 2 · 22 Jun 2020 · 7,610 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetection of tet Gene in Multidrug-Resistant Salmonella spp. Isolates from Layers and Broiler BreedersAgustin Indrawati, Leila Nur Azizah, I Wayan Teguh Wibawan et al.The present study was conducted with the following aims: (i) To detect the multidrug-resistant Salmonella spp. isolates recovered from faeces, litter and drinking water in layers and broiler breeders’ farms and (ii) to carried out the detection of the tet gene. A total of 21 Salmonella isolates were subjected to Polymerase Chain Reaction (PCR) assay to determine the presence of … …American Journal of Animal and Veterinary Sciences, Vol. 15, Issue 2 · 17 Jun 2020 · 7,007 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDetection of Commercial Fraud in Processed Meat Products Using Rapid TechniquesAli M. Ahmed, Takwa H. Ismail, Reham R. Abouelmaatti et al.Meat adulteration with different species has become a considerable problem worldwide. It means that meat products contain undeclared meat species; so, the meat ingredients are not consistent with the label. It has direct impact on public health, religious faith, fair-trades and food safety standers. This study focused on species adulteration detection in meat products by applying accurate, slandered and fast … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 7,817 accessesView PDF Cite