Alcohol Intubation and Synergistic Effect of Folic Acid and Egg Yolk Powder Solution on Alcohol Dehydrogenase [ADH] Class I Gene Expression and Single Nucleotide Polymorphism Frequency in Parent and Prenatal Alcohol Exposed Wistar Rats

Point mutation detections in alcohol metabolizing genes could unravel molecular targets of gestational alcohol and epigenetic remodeling capacity of maternal nutrition, which underscores growth and health endpoints in Pre­natal Alcohol Exposed (PAE) fetuses. gene expression was assayed using sequenced target and internal control gene primers in a two-step thermal cycling process from isolated RNA in purely bred alcohol male and female Norway rats weighing between 200-220 g. They were mated and grouped as follows: (A) Distilled water only [negative control)., (B) Local gin only, (C) Local gin supplemented with egg yolk solution, (D) Local gin supplemented with egg yolk solution and folic acid (E) EtOH only (positive control). Equal doses of 3.0 mL/kg/bw were administered before, during, and after gestation via oral gavage. ADHI gene subtypes in the pups of mated rats were analyzed by the methods used in the parental stocks. The PCR pro­ducts were electrophoresed on a 2.5% agarose gel while bioinformatics databases for SNP detection were used for the sequenced data analyses in the alcohol-dosed parent and PAE rats. a total number of 35,838 bp coding DNA of the ADH1 gene were sequenced in the experimental rats with 115 random synonymous and non-synonymous SNP distributions. Parent-of-origin inheritance pattern of ADHI was confirmed in PAE neo­nates while SNPs frequency in sequence data of ADHI subtypes in groups' B-E dicts func­tional alcohol effects and changes in resultant pro­tein. Hence, this study provides evidence for paternal and maternal contributions and nutrient-gene interactions in PAE rats.