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35 results for “Expression Vector”
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Research ArticleOpen accessIn the abstractProduction of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coliDessy Natalia, Christian Heryakusuma, Fernita Puspasari et al.Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 10 Oct 2018 · 10,346 accessesView PDFCite
Research ArticleOpen accessIn the abstractPerformance Analysis of LDA, AdaBoost and Ensemble Bag Classifiers for Automatically Recognizing Nine Common Facial ExpressionsM.D. Sumithra, M. Abdul RahimanFacial expression recognition holds a prominent role in today’s digital world with more Human –Computer Interaction happening in day to day life. Successful identification of facial expressions needs extraction of descriptive attributes from the active facial patches and accurate classification. This paper is presented with the comparison of three multi class classifiers namely Linear Discriminant Analysis, AdaBoost and Ensemble bag. … …Journal of Computer Science, Vol. 14, Issue 9 · 27 Sep 2018 · 7,787 accessesView PDFCite
Research NotesOpen accessIn the abstractAn Artificial Design Technique to Optimize Signal PeptideGao Cui-Fang, Wang Sen, Tian Feng-Wei et al.To determine optimal artificial signal peptide candidates for the possibility of creating high levels of secretion of heterologous proteins, substitution and redesign of amino acid sequences in the H-domain of the signal peptide was theoretically attempted. The method was based on comprehensive score matrix and Markov transfer matrix, which can make the artificial sequences maintain the structural characteristics and original … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 3 · 21 Jul 2017 · 8,063 accessesView PDFCite
Research ArticleOpen accessIn the abstractDimensionality Reduction using Principal Component Analysis for Cancer Detection based on Microarray Data ClassificationAdiwijaya, Untari N. Wisesty, E. Lisnawati et al.Cancer is one of the most deadly diseases in the world. The International Agency for Research on Cancer (IARC) noted 14.1 million new cancer cases and 8.2 million deaths from cancer in 2012. In the last few years, DNA microarray technology has increasingly been used to analyze and diagnose cancer. Analysis of gene expression data in the form of microarray … …Journal of Computer Science, Vol. 14, Issue 11 · 15 Nov 2018 · 9,930 accessesView PDFCite
EditorialOpen accessIn the abstractCRISPR-CAS SYSTEM: A FEASIBLE SOLUTION FOR GETTING RID OF PERSISTENT VIRAL INFECTION?Zhaohua ZhongMany viruses, including DNA and RNA viruses, can persistently replicate in the infected cells and cause a prolonged damage. Herpes viruses can be latent in its host cells. Human Immunodeficiency Virus (HIV) reversely transcripts its RNA genome to DNA and integrates to the host’s chromosome. Hepatitis B Virus (HBV) and Human Papilloma Virus (HPV) persistently stay in the host cells … …Current Research in Virology, Vol. 3, Issue 1 · 10 Nov 2014 · 12,614 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Recombinant Proteins in Insect CellsChristian Kollewe, Andreas VilcinskasAmong the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive post-translational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression Vector System (BEVS). We discuss the different strategies … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 3 · 21 Sep 2013 · 13,742 accessesView PDFCite
Research ArticleOpen accessIn the abstractHigh-Yield Production of Bioactive Flatfish (Paralichthys olivaceus) Myostatin-1 Prodomain in Escherichia coliIl Shik Shin, Hyung-Joo Jin, Jeong Hwan Kim et al.Myostatin Propeptide (MstnPro), the N-terminal part of Mstn precursor molecule, binds to Mstn and suppress its activity. Mstn is a potent negative regulator of skeletal muscle growth in many animal species, thus MstnPro has a great potential to be used as an agent to enhance skeletal muscle growth in animal species. Bioactive fish Myostatin-1 Propeptide (Mstn1Pro) proteins were recently expressed … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 3 · 20 Sep 2012 · 8,012 accessesView PDFCite
Research ArticleOpen accessIn the abstractA NAPIN PROMOTER ACTIVATES GENE EXPRESSION IN DEVELOPING SEEDS OF LESQUERELLA FENDLERIGrace Q. Chen, Jiann-Tsyh LinLesquerella fendleri produces industrial useful Hydroxy Fatty Acids (HFA) in seed oil. To improve oil and HFA of L. fendleri, it is desirable to use of seed-specific promoters to control the expression of target genes by genetic engineering. A seed-specific promoter fragment, -397 to -1 of a napin gene (PnapA) from Brassic napus was isolated by PCR and constructed to … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 18 Sep 2012 · 7,890 accessesView PDFCite
Research ArticleOpen accessIn the abstractTransformation of Lesquerella Fendleri with the New Binary Vector pGPro4-35SGrace Q. Chen, Roger Thilmony, Jiann-Tsyh LinProblem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,425 accessesView PDFCite