Author

Jiann-Tsyh Lin

Western Regional Research Center, United States

3 articles in our journals

Articles where this person is an author, newest first. People are matched by their name as printed (no ORCID iD is recorded for them), not by name alone; search the name as text to find namesakes too.

Research ArticleOpen accessA NAPIN PROMOTER ACTIVATES GENE EXPRESSION IN DEVELOPING SEEDS OF LESQUERELLA FENDLERIGrace Q. Chen, Jiann-Tsyh LinLesquerella fendleri produces industrial useful Hydroxy Fatty Acids (HFA) in seed oil. To improve oil and HFA of L. fendleri, it is desirable to use of seed-specific promoters to control the expression of target genes by genetic engineering. A seed-specific promoter fragment, -397 to -1 of a napin gene (PnapA) from Brassic napus was isolated by PCR and constructed to … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 18 Sep 2012 · 7,890 accessesView PDF Cite

Research ArticleOpen accessTransformation of Lesquerella Fendleri with the New Binary Vector pGPro4-35SGrace Q. Chen, Roger Thilmony, Jiann-Tsyh LinProblem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,425 accessesView PDF Cite

Research ArticleOpen accessUse of Quantitative Polymerase Chain Reaction for Determining Copy Numbers of Transgenes in Lesquerella fendleriGrace Qianhong Chen, Jiann-Tsyh LinProblem statement: In transgenic plants, the number of transgene copies could greatly influence the level of expression and genetic stability of the target gene, thus it is important to develop an efficient method for accurate estimation of transgene copies. The quantitative Polymerase Chain Reaction (qPCR) technique is becoming more efficient nowadays to determine copy numbers of transgenes in transgenic plants, … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,191 accessesView PDF Cite