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Research ArticleOpen accessIn the abstractUse of Cross-linking to Assess Subunit Interaction of Recombinant Human Coproporphyrinogen OxidaseJason R. Stephenson, Nancy E. Thomas, Jon A. Friesen, Marjorie A. JonesTo provide further evidence for a dimeric form of coproporphyrinogen oxidase reported using the conventional hydrodynamic methods, bifunctional cross-linkers were incubated with purified, recombinant human coproporphyrinogen oxidase to determine subunit interaction in solution. The use of cross-linkers provides an effective way to demonstrate subunit association and allows for assessment of activity upon covalent cross-linking. Following incubation with selected cross-linkers, enzyme … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,597 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effects of Chinese Traditional Processing Method on Components in Semen Sinapis AlbaeLifang Liu, Hui Zhou, Suqin Sun et al.Techniques of infrared (IR) spectrophotometry, High Performance Liquid Chromatography (HPLC) and HPLC-mass spectrometry are employed in this study to reveal the changes of the components in Semen sinapis Albae during the Chinese traditional processing. While the degradation of proteins and polysaccarides can be observed, sinapine in this drug can be known to decompose immediately after 15 mins during processing. A … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 2 · 30 Jun 2005 · 6,815 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of Transparent Conductive Thin Films of In 2O 3:Sn by Spray Pyrolysis TechniqueSeyed Mohammad Rozati, Tohid GanjHighly conductive transparent In2O3:Sn (ITO) thin films were prepared onto glass substrate using the spray pyrolysis technique. It is observed that these films are polycrystalline with the preferred orientation of [400]. The extent of this orientation increases with substrate temperature. The results show that films with a sheet resistance as low as 35Ω/cm2 and with a transmittance as high as … …American Journal of Applied Sciences, Vol. 2, Issue 6 · 30 Jun 2005 · 6,766 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Gamma Irradiation on the Structure Diffusion Coefficient of Co-Zn Doped FerriteDalal Mohammed HemedaA series of the ferrite system Co0.6Zn0.4MnxFe2-xO4 with (x = 0.7, 0.8, 0.9 and 1) were prepared by general ceramic method. The X-ray diffraction patterns confirmed the formation of the cubic single phase structure. The lattice parameter and the crystal size increased after irradiation which was attributed to the formation of ferrous ions of larger radius than that of ferrous … …American Journal of Applied Sciences, Vol. 2, Issue 5 · 31 May 2005 · 6,731 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Laser Irradiation on the Structural and Electrical Properties of Co-Zn Ferrite Thin FilmNehad M. TashtoushThin films of irradiated and non-irradiated Co-Zn ferrite was prepared by the thermal evaporation method on a glass substrate. The samples were annealed at 200°C, 400°C and 600°C for 1hr. The XRD analysis of all kinds of films, prepared for this study, shows that the spinel phase is almost completed after annealing. The change of the XRD after annealing is … …American Journal of Applied Sciences, Vol. 2, Issue 4 · 30 Apr 2005 · 7,039 accessesView PDFCite
Research ArticleOpen accessIn the abstractResidual Defects in Silicon Implanted with Boron and Phosphorous IonsM. JadanAccumulation of radiation defects in Si implanted with B+ or P+ ions, and formation of the residual extended defects (dislocation loops, rod-like defects) in the process of successive thermal treatment have been studied. The anomalies observed in the formation of the extended defects are associated with the elastic stresses in the damaged regions affecting the process of radiation defects clustering. …American Journal of Applied Sciences, Vol. 2, Issue 4 · 30 Apr 2005 · 6,935 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacterization of Reconstructed Basins Using Pattern Spectrum ProcedureP. RadhakrishnanSeveral classical and Fractal binary shapes, which are akin to geophysical shapes such as basins, lakes, and pore-grain spruce, are analyzed and characterized by employing various mathematical morphological transformations, and methods. By employing rhombus, square and octagon structuring elements, these shapes are decomposed into their skeletal networks and their corresponding skeletal network subsets are dilated to the respective degree by … …American Journal of Applied Sciences, Vol. 2, Issue 4 · 30 Apr 2005 · 6,933 accessesView PDFCite
Research ArticleOpen accessIn the abstractFactors Associated with Syringe Sharing Among Users of a Medically Supervised Safer Injecting FacilityEvan Wood, Mark W. Tyndall, Jo-Anne Stoltz et al.Vancouver, Canada recently opened a medically supervised safer injecting facility (SIF) in an effort to reduce HIV and overdose risk and public injection drug use. We sought to examine factors associated with syringe sharing among SIF users. SIF users were randomly recruited into a prospective cohort of SIF users known as the Scientific Evaluation of Supervised Injecting (SEOSI) cohort. We … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 10,764 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvidences of Apoptosis in Hepatitis C Virus-Infected Hepatocytes and Peripheral Blood Monocuclear Cells in the Absence of Liver InjuryViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.Understanding the mechanism of Hepatitis C Virus (HCV) pathogenesis is an important part of HCV research. In this study, the presence of apoptosis in HCV-infected liver and Peripheral Blood Mononuclear Cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA was investigated. The samples obtained from 21 patients were studied by in situ Hybridization (ISH), Immunofluorescence, TUNEL … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 6,984 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvidences of Hepatitis C Virus Replication in Hepatocytes and Peripheral Blood Monocuclear Cells from Patients Negative for Viral RNA in SerumViviana Falcón, Nelson Acosta-Rivero, Mineko Shibayama et al.In this study, we examined the expression of hepatitis C virus (HCV) components in hepatocytes and peripheral blood mononuclear cells (PBMC) from patients positive for anti-HCV antibodies and negative for serum HCV-RNA. The samples obtained from 25 randomly selected patients (13 of 25 patients showed no histological evidences of chronic hepatitis and had normal serum ALAT and GGTP levels) were … …American Journal of Infectious Diseases, Vol. 1, Issue 1 · 31 Mar 2005 · 7,173 accessesView PDFCite