4 results for “transgenes”

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Research ArticleOpen accessEvery word in the titleUse of Quantitative Polymerase Chain Reaction for Determining Copy Numbers of Transgenes in Lesquerella fendleriGrace Qianhong Chen, Jiann-Tsyh LinProblem statement: In transgenic plants, the number of transgene copies could greatly influence the level of expression and genetic stability of the target gene, thus it is important to develop an efficient method for accurate estimation of transgene copies. The quantitative Polymerase Chain Reaction (qPCR) technique is becoming more efficient nowadays to determine copy numbers of transgenes in transgenic plants, … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,191 accessesView PDF Cite

Research ArticleOpen accessIn the abstractTransformation of Lesquerella Fendleri with the New Binary Vector pGPro4-35SGrace Q. Chen, Roger Thilmony, Jiann-Tsyh LinProblem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,425 accessesView PDF Cite

Research ArticleOpen accessIn the abstractCharacterization of Substrate-Borne Vibrational Signals of Euschistus servus (Heteroptera: Pentatomidae)Brittany Lampson, Young Han, Ahmad Khalilian et al.Problem Statement: Stink bugs were not major pests of cotton in the southeastern United States until cotton varieties containing Bacillus thuringiensis (Bt) transgenes came into common use, enabling reductions in insecticide applications against highly destructive lepidopteran pests. To maintain the benefits of reduced insecticide applications, it would be advantageous to detect and target isolated populations of stink bugs before they … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 1 · 31 Mar 2010 · 8,272 accessesView PDF Cite

EditorialOpen accessIn the abstractCRISPR-CAS SYSTEM: A FEASIBLE SOLUTION FOR GETTING RID OF PERSISTENT VIRAL INFECTION?Zhaohua ZhongMany viruses, including DNA and RNA viruses, can persistently replicate in the infected cells and cause a prolonged damage. Herpes viruses can be latent in its host cells. Human Immunodeficiency Virus (HIV) reversely transcripts its RNA genome to DNA and integrates to the host’s chromosome. Hepatitis B Virus (HBV) and Human Papilloma Virus (HPV) persistently stay in the host cells … …Current Research in Virology, Vol. 3, Issue 1 · 10 Nov 2014 · 12,614 accessesView PDF Cite