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16 results for “thawing”
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Research ArticleOpen accessEvery word in the titleEffect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer SystemMasindi Lottus Mphaphathi, Tshimangadzo Lucky NedambaleThe aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 1 · 27 Jan 2021 · 6,596 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of the Tensioactive Agent SDS on Extender’s Boar Semen Viability after ThawingMaria Helena Moreira da Silva, Loide Valadão, Fernando Moreira da SilvaEffects of the surfactant Sodium Dodecyl Sulfate (SDS) on the motility and viability of boar semen after thawing were evaluated. For such purpose, semen of twelve adult boars was collected twice a week, the rich portion of this semen was separated and frozen with different concentrations of SDS (0; 2.5; 5; 10; 20 and 40 mM). For freezing the semen … …American Journal of Applied Sciences, Vol. 18, Issue 1 · 20 Jan 2021 · 7,797 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleFreezing and Thawing Durability of Very High Strength ConcreteSameer Hamoush, Miguel Picornell-Darder, Taher Abu-Lebdeh, Ahmed MohamedProblem statement: The newly developed Very High Strength Concrete (VHSC), having compressive strengths of 29 ksi and flexural strengths of 6 ksi, represents a breakthrough in concrete technology. Study to further enhance the properties of this new concrete is continuing. Approach: The objective of this study is to investigate the effect of exposing Very High Strength Concrete (VHSC) specimens to … …American Journal of Engineering and Applied Sciences, Vol. 4, Issue 1 · 28 Jan 2011 · 8,823 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimizing Boar Epididymal Sperm Preservation: Comparative Analysis of Slicing Float-Up vs. Flushing and BTS vs. Andromed® Extenders on Sperm Motility and Morphometry TraitsMamonene Angelinah Thema, Ntuthuko Raphael Mkhize, Gerhard van der Horst et al.Epididymal sperm collection represents a valuable strategy for preserving genetics from deceased or castrated boars of superior breeding value. The two primary collection methods-slicing float-up (high sperm yield) and flushing (low blood contamination risk)-differ in sperm recovery efficiency, while semen extenders provide cryoprotective nutrients that protect against cold shock during preservation. This study compared the effects of slicing float-up versus … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 4 · 14 Mar 2026 · 1,800 accessesView PDFCite
Literature ReviewOpen accessIn the abstractLiterature Study on Factors Influencing the Biochemical Analytes Stability in Blood, Serum and Plasma: Systematic ReviewSamia El Mahi, Asmae Tantane, Sanaa Ait Hamou, Said OubraimBlood testing represents an essential phase in diagnosis and treatment. It is important to comprehend the biochemical analytes' stability. This research gathers information from different journals that have investigated the stability of biochemical analytes under different conditions. This research aims to collect and analyze data about conditions that promote stability, conditions that lead to instability, and how stability varies in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 21 Aug 2024 · 5,198 accessesView PDFCite
Research ArticleOpen accessIn the abstractSupplementation with Different Concentrations of Dithiothreitol and Glutathione During Cryopreservation of Semen from Large White BoarsMahlatsana Ramaesela Ledwaba, Masindi Lottus Mphaphathi et al.The study aims to compare different concentrations (0, 2.5, 5, 7.5 and 10 mM) of antioxidants, Dithiothreitol (DTT) and Glutathione (GSH), on sperm parameters following the freeze-thawing of semen from large white boars. Three large white boars were selected for semen collection and the collected semen was sent to the lab for analysis. Following dilution with Beltsville thawing solution and … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 3 Jun 2024 · 5,520 accessesView PDFCite
Research ArticleOpen accessIn the abstractFrozen Semen Quality of Kolbroek Boars Concerning Glutathione ConcentrationsLerato Deirdre Sehlabela, Masindi Lottus Mphaphathi et al.Glutathione (GSH) is an antioxidant that is being studied for its ability to improvecellular responses to semen preservation challenges. The research goal aimed toevaluate the impact of introducing different glutathioneconcentrations to boar semen freezing extender (0, 1, 5, and 10 mM) onthe Kolbroek boar sperm's quality after being frozen and thawed. For 2 h, thecollected sperm-rich fraction was chilled at … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 1 Apr 2023 · 6,008 accessesView PDFCite
Research ArticleOpen accessIn the abstractEvaluation of Japanese Quail Egg-Yolk Extender in Cryopreservation of Cockerel SemenRantloko Rolly Maapola, Khathutshelo Agree Nephawe, Tshimangadzo Lucky Nedambale et al.The purpose of this study was to examine the effects of quail egg yolk extenders in the cryopreservation of cockerel semen. Ten Venda cockerels were chosen randomly and semen was collected twice a week with a two-day rest period using an abdominal massage technique. Immediately following semen collection, semen samples were evaluated for microscopic parameters (sperm cell motility, viability, morphology, … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 25 Mar 2023 · 5,644 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Intracellular Reactive Oxygen Species in Several Spermatozoa Activity in Indonesian Ongole Bull Cryopreserved SpermKurniawan Dwi Prihantoko, Asmarani Kusumawati, Mulyoto Pangestu et al.Certain factors may affect sperm motility and integrity in a freezing process. Reactive Oxygen Species (ROS) is essential in damaging the sperm, as the previous study revealed that ROS is a major cause damaging the membrane of spermatozoa. This study aimed to understand ROS production and its interaction within the motility, mitochondrial activity and DNA damage to the cryopreservation process … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 1 · 26 Jan 2022 · 6,846 accessesView PDFCite
Research ArticleOpen accessIn the abstractStudy the Effect of Extracted Low-Density Lipoprotein from Egg Yolk on the Cryopreservation of Nguni Bull SemenMduduzi M. Tshabalala, Masindi Lottus Mphaphathi, Khathutshedzo A. Nephawe et al.The mechanism on how Egg Yolk (EY) helps sperm to survive the low temperature outside the animal body during cryopreservation and thawing process is not yet known. It is usually believed that the Low-Density Lipoprotein (LDL) is the major component that provides protection. The present study was undertaken to assess the cryo-protective effect of different concentrations (6, 8, 10 and … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 3 · 13 Sep 2021 · 6,921 accessesView PDFCite