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79 articles for “polymerase chain reaction”
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Research ArticleOpen accessPhrase in the titleAnalysis of the Utility of MPT64, Drug Susceptibility Testing and Tuberculosis/Nontuberculous Mycobacteria Reverse Transcription Polymerase Chain Reaction for Tuberculosis Diagnosis and Efficiency Compared with Traditional CultureHo Keun Choi, Ga Yeon Kim, Tae Soung Kim, Jae Kyung KimDefining the molecular mechanisms underlying the dormant state of Mycobacterium tuberculosis (MTB) is essential for developing more effective treatments for tuberculosis (TB) infection. We investigated the positivity and resistance rates of TB tests on 6,174 samples referred to a single hospital. Of these, 342 cases (5.5%) were confirmed as positive by acid-fast bacilli staining, 243 cases (71.1%) were detected as … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 5 Dec 2024 · 4,206 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleUse of Quantitative Polymerase Chain Reaction for Determining Copy Numbers of Transgenes in Lesquerella fendleriGrace Qianhong Chen, Jiann-Tsyh LinProblem statement: In transgenic plants, the number of transgene copies could greatly influence the level of expression and genetic stability of the target gene, thus it is important to develop an efficient method for accurate estimation of transgene copies. The quantitative Polymerase Chain Reaction (qPCR) technique is becoming more efficient nowadays to determine copy numbers of transgenes in transgenic plants, … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 3 · 30 Sep 2010 · 8,191 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleDNA Code Word Design for DNA Computing with Real-Time Polymerase Chain ReactionMuhammad Faiz Mohamed Saaid, Zuwairie Ibrahim, Mohamad Shukri Zainal Abidin et al.Problem statement: A number of DNA computing models to solve mathematical graph problem such as the Hamiltonian Path Problem (HPP), Traveling Salesman Problem (TSP), and the Shortest Path Problem (SPP), have been proposed and demonstrated. Normally, the DNA sequences used for the computation should be critically designed in order to reduce error that could occur during a computation. We have … …Journal of Computer Science, Vol. 5, Issue 1 · 31 Jan 2009 · 7,502 accessesView PDFCite
Research ArticleOpen accessPhrase in the titlePolymerase Chain Reaction Detection of Pasteurella multocida Type B: 2 in Mice Infected with Contaminated River WaterFaez Firdaus Jesse Abdullah, Mohammed Muqdad Khaleel, Lawan Adamu et al.Hemorrhagic septicemia is an acute, deadly disease of cattle and buffaloes associated with colossal economic loss in the livestock industry in the Asian regions particularly Malaysia. Therefore, this study was conducted to investigate on the Polymerase chain reaction detection of Pasteurella multocida type B: 2 in mice inoculated through different routes with river water contaminated with infected mice carcasses. Sixty … …American Journal of Animal and Veterinary Sciences, Vol. 8, Issue 3 · 17 Sep 2013 · 7,392 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleDetection of Polymorphism at the Insulin Like Growth Factor-I Gene in Mazandaran Native Chicken using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism MethodHossein Ali Abbasi, Mohsen KazemiProblem statement: Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in chickens. The Insulin like Growth Factor-I (IGF1) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. Approach: In the present study polymorphism of the promoter and 5' untranslated region of IGF-1 gene of Mazandaran … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 2 · 8 Aug 2011 · 7,627 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleUsing Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction of Barbodes spp. in ThailandKeeravit Petjul, Aphidech Sangdee, Bungorn Thaewnon-ngiwProblem statement: The genetic diversity of Barbodes species in Thailand is presently unavailable. The objectives of this study were to study external morphology and genetic diversity using Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) of three Barbodes species; Barbodes altus, B. gonionotus and Barbodes sp. Approach: Two of twelve screened decanucleotide primers (OPA07 and UBC122), were selected for the genetic … …OnLine Journal of Biological Sciences, Vol. 11, Issue 1 · 6 Jul 2011 · 8,085 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleEpidemiology and Detection of Bacterial Diarrhea Using Multiplex Polymerase Chain Reaction, Focusing on Campylobacter spp.Eun Ju Oh, Tae Su Jang, Jung Min Park, Jae Kyung KimThis study assessed the clinical characteristics, epidemiological trends, and changes associated with major pathogens causing gastrointestinal inflammation. We retrospectively analyzed the test results of hospitalized patients who were administered a mPCR test for diarrhea causing bacteria between June 2010 and December 2019 at the Cheonan Dankook University hospital in the Republic of Korea and identified clinical characteristics and epidemiological trends … …American Journal of Biochemistry and Biotechnology, Vol. 19, Issue 1 · 27 Mar 2023 · 4,979 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleCharacterization of Bovine Calpastatin Gene in Nelore Cattle Using Polymerase Chain Reaction-Restricted Fragment Length PolymorphismsAlireza Majidi, Jothi Malar Panandam, Awis Qurni Sazili, Siti Shapor SirajProblem statement: In beef cattle production, of meat quality and carcass traits are important. Traditionally beef cattle breeding programs unfortunately are time consuming and also recording of carcass and growth traits need heavy cost, Approach: Marker Assisted Selection (MAS) should be utilized in beef herds, along with economically important phenotypic traits, for genetic progress to made with respect to improving … …American Journal of Animal and Veterinary Sciences, Vol. 4, Issue 4 · 31 Dec 2009 · 7,329 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleAssessment of Genotoxic Activity of Para-nitrophenol in Higher Plant Using Arbitrarily Primed- polymerase Chain Reaction (AP-PCR)Mohamed R. EnanParanitrophenol is a common toxic environmental pollutant; the aim of this study was to evaluate the potential of the DNA fingerprinting by AP-PCR assay to detect the DNA damage in the common bean (Phaseolus vulgaris) exposed to different concentrations of paranitrophenol (4.0-720 nM). The changes occurring in the fingerprint patterns were likely to be the result of paranitrophenol-induced DNA damage. … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 2 · 30 Jun 2007 · 7,064 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleApplications of Real-time Reverse Transcription Polymerase Chain Reaction in Clinical Virology Laboratories for the Diagnosis of Human DiseasesR. Manojkumar, V. MrudulaEarly diagnosis that prevents further expansion of the causative agent and thereby aids control and eradication of infectious agents is critical for countries trying to obtain a particular disease free status. In these diagnostics speed is paramount, including the assay’s applicability, sensitivity, specificity, cost effectiveness and patentability. Real-time Reverse Transcription PCR (rRT-PCR) has revolutionized the field of molecular biology and … …American Journal of Infectious Diseases, Vol. 2, Issue 4 · 31 Dec 2006 · 6,553 accessesView PDFCite