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30 results for “immobilized”
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Research ArticleOpen accessEvery word in the titleStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDegradation of Textile Dyes Ponceau-S and Sudan IV Using Recently Developed Photocatalyst, Immobilized Resin Dowex-11R. C. Meena, Ram Babu Pachwarya, Vijay Kumar Meena, Shakuntla AryaProblem statement: In present study, we selected a model dyes Ponceau S and Sudan IV, to test a recently developed photo catalyst methylene blue immobilized resin dowex-11. Approach: This is a light-activated process that has been successfully applied to remove organic and inorganic dyes of textile industries. Results: The reactor, made of glass slides (tubes) coated with a thin-film of … …American Journal of Environmental Sciences, Vol. 5, Issue 3 · 30 Jun 2009 · 7,054 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImmobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide DetectionChadaporn Pootawee, Witsanu Senbua, Jesdawan WichitwechkarnAs the result of the extensive use and high contamination of organophosphate (OP) insecticides in agricultural products and the environment, especially in developing countries, a simple and convenient means for OP detection and screening was investigated. Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 18 Sep 2018 · 7,673 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleBiosorption of Textile Dye Using Immobilized Bacterial (Pseudomonas aeruginosa) and Fungal (Phanerochate chrysosporium) CellsNatarajan Saravanan, Tiruvenkadam Kannadasan, Chiya Ahmed Basha, Veerasamy ManivasaganWastewater containing dyes presents a serious problem due to its high toxicity which leads to creating enormous environmental pollution and ecological hazards. Therefore the removal of the high stable dyes from the textile effluents is of major importance. The purpose of this study is to remove the reactive dye Procion Blue 2G from textile dye solution by biosorption process using … …American Journal of Environmental Sciences, Vol. 9, Issue 4 · 16 Sep 2013 · 7,350 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleHydrogen Peroxide Biosensor Based on the Direct Electrochemistry of Myoglobin Immobilized in Poly-3-Hydroxybutyrate FilmXiang Ma, Rongwu Yang, Genxi LiDirect electrochemistry of myoglobin (Mb) was observed in a stable film composed of a natural lipid polymer (poly-3-hydroxybutyrate) and Mb, the film of which was modified on a pyrolytic graphite electrode. The apparent formal potential of Mb was at about -260 mV in an acetate buffer solution with pH 5.0. Moreover, Mb in the polymer film exhibited catalytic activity towards … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 1 · 31 Mar 2005 · 6,872 accessesView PDFCite
Research ArticleOpen accessIn the keywordsPotential Applications of Chitosan Nanoparticles as Novel Support in Enzyme ImmobilizationHoda Jafarizadeh Malmiri, Mohammad Ali Ghaz Jahanian, Aydin BerenjianChitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,302 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSurface Modification of Polystyrene Beads by Ultraviolet/Ozone Treatment and its Effect on Gelatin CoatingA. N. Yusilawati, M. Maizirwan, M. S. Hamzah et al.Problem statement: Polystyrene failed to provide any reactive functionality of surface hydrophilicity that is capable of binding proteins. It is known that polystyrene must be chemically modified to make its surface amenable to covalent crosslinking with protein. Approach: The aim of this study was to investigate the effects of UV/ozone treatment on gelatin coating. The surfaces of microsize polystyrene beads … …American Journal of Applied Sciences, Vol. 7, Issue 6 · 30 Jun 2010 · 8,844 accessesView PDFCite
Research ArticleOpen accessIn the abstractPotentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer ImmobilizationZhainagul Kakimova, Klara Zharykbasova, Aitbek Kakimov et al.Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection of these contaminants, a potentiometric biosensor was developed for the quantification of Pb²⁺ and Cd²⁺ ions in milk and dairy products. The biosensor functions by measuring the change … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 8 Feb 2026 · 1,480 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiodegradation of 2, 4 DichlorophenolTaghreed Al-Khalid, Muftah H. El-NaasThe present study focuses on the optimization of process parameters of the aerobic biodegradation of 2, 4 Dichlorophenol (2, 4 DCP) by a commercial strain of P. putida immobilized in PVA gel matrix, using design of experiments by Response Surface Methodology (RSM). The degradation rates obtained from these experiments were used to evaluate the effects of the main factors and … …American Journal of Engineering and Applied Sciences, Vol. 10, Issue 1 · 12 Mar 2017 · 7,390 accessesView PDFCite