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6 results for “extracellular lipase”
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Research ArticleOpen accessEvery word in the titleImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Production of Extracellular Alkaline Lipase by an Improved Strain of Pseudomonas aeruginosa MTCC 10,055Deepali Bisht, Santosh Kumar Yadav, Nandan Singh DarmwalProblem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation … …American Journal of Applied Sciences, Vol. 9, Issue 2 · 24 Nov 2011 · 8,760 accessesView PDFCite
Research ArticleOpen accessIn the keywordsProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCharacterization and Wash Performance Analysis of Microbial Extracellular Enzymes from East Calcutta Wetland in IndiaRamesh Malathu, Sanhita Chowdhury, Madhusmita Mishra et al.Extracellular protease from a novel bacterial isolate showing maximum similarity of 98.22% with Microbacterium luteolum was obtained from East Calcutta Wetland, India. It showed compatibility with commercial detergents. The enzyme retains more than 60% of its activity between 6.0 to 10.5 pH. The maximum activity is at pH 7.5 with 71% activity at pH 10.0 and 10.5. The protease retained … …American Journal of Applied Sciences, Vol. 5, Issue 12 · 31 Dec 2008 · 8,727 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessAll words, across the recordCondoms-As Sources of Extracellular Enzyme Producing MicrobesMadhusmita Mishra, Poulomi Nandy, Sarvani Debsarma et al.Problem statement: Condoms are widely used birth control measures which are made of natural latex or synthetic materials like polyurethane or polyisoprene as matrix with lubricants added to make them ready to use commodities. Absence of sterilization method along with presence of lubricant like petroleum jelly (that are applied on the outer surface), prompted the investigators to look for microbes … …Current Research in Microbiology, Vol. 1, Issue 2 · 31 May 2011 · 8,985 accessesView PDFCite