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11 articles for “biocatalyst”
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Research ArticleOpen accessEvery word in the titleAn Efficient Production of Oligosaccharides by a Reaction using Whole Mammalian Cells as BiocatalystsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaProblem statement: Mammalian cells were used for the production of oligosaccharides by saccharide primer method. However, because cells in culture are used, productivity of oligosaccharides is low. Approach: In saccharide primer method employing cells under culture, glycosylation was performed only by mixing the collected cells in reaction mixtures. Saccharide primer, 12-azidododecyl β-lactoside that mimics lactosylceramide (LacCer), was incubated with various … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,439 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnhancement of Geobacillus thermodenitrificans Lipase Activity and Binding Affinity by Acetone Treatment: Kinetic and in silico InvestigationsAmmar Khazaal Kadhim Almansoori, Alyaa Abdulhussein Alsaedi et al.Lipase Geobacillus thermodenitrificans (LipGt) is a thermostable enzyme that has an optimal activity temperature of 65°C. It can tolerate various organic solvents and has an improved activity, especially in the presence of acetone. In this study, in-vitro and in-silico studies were carried out to investigate the effect of acetone in enhancing the kinetic and binding affinity of LipGt. The LipGt … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 10 Oct 2024 · 4,817 accessesView PDFCite
Research ArticleOpen accessIn the abstractYeast Immobilization on Peanut Hulls for Potential Application in Beer FermentationWaldir D. Estela-Escalante, Sergio Rosales-Mendoza, Mario Moscosa-SantillánThe potential of using peanut hulls as support material for immobilization of yeast cells during beer fermentation was evaluated. After treatment of peanut hulls with 0.5% (w/v) NaOH, FTIR analysis revealed an important change on its chemical composition. For instance, the proportion of lignin decreased and as a result the adsorption of yeast cells onto the support material during fermentation … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 22 Jul 2019 · 8,375 accessesView PDFCite
Research ArticleOpen accessIn the abstractEnzymatic Synthesis of Palm Alkyl Ester Using Dialkyl Carbonate as an Alkyl DonorsRoila Awang, Choo Yuen MayProblem statement: Though efficient in terms of reaction yield and time, the chemical approach to synthesizing alkyl ester has drawback such as difficulties in the recovery of glycerol and the need for removal of salt residue. On the other hand, biocatalyst allow for synthesis of specific alkyl esters and easy recovery of glycerol. However, the solvent-free alcoholysis, does not give … …American Journal of Applied Sciences, Vol. 7, Issue 8 · 31 Aug 2010 · 7,235 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Biodiesel by Enzymatic Transesterification: ReviewA.E. Ghaly, D. Dave, M.S. Brooks, S. BudgeProblem Statement: The research on the production of biodiesel has increased significantly in recent years because of the need for an alternative fuel which endows with biodegradability, low toxicity and renewability. Plant oils, animal fats, microalgal oils and waste products such as animal rendering, fish processing waste and cooking oils have been employed as feedstocks for biodiesel production. In order … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 21,467 accessesView PDFCite
Research ArticleOpen accessIn the abstractModification of Artificial Oliogosaccharides in Recombinant Escherichia coli CellsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaArtificial oligosaccharides were modified using recombinant Escherichia coli cells that overexpress sialidase. Based on the principle of the saccharide primer method by using bacterial cells overexpressing enzymes related to oligosaccharide modification. Problem statement: It is very hard to obtain oligosaccharides, because they have complex and diverse structures with different linkage patterns and monosaccharide components. Approach: It had been known that … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,802 accessesView PDFCite
Research ArticleOpen accessIn the abstractStabilization of Tyrosinase-Bovine Serum Albumin Crystals by GlutaraldehydeD. Norouzian, A. Akbarzadeh, M.H. Abnosi et al.Tyrosinase and bovine serum albumin were co-crystallized by saturated ammonium sulfate solution(65%) and 20% polyethylene glycol ( PEG) 6000 and n-propanol as co-solvents .The obtained crystals were cross linked by glutaraldehyde solution(1% v/v).Polyethylene glycol 6000 was found to be better co-solvent than n-propanol. The developed biocatalyst could be recycled 6 times without further loss of tyrosinase activity. No loss of … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,194 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessIn the abstractOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDFCite
Review ArticleOpen accessIn the abstractLupane-Type Triterpenoids BiotransformationZhumadilov Sayat Sagatovich, Bakibaev Abdigali Abdimanapovich, Zhadan Konstantin Vasilevich et al.A lot of scientific material has been accumulated in recent years about the biotransformation processes of lupine-type triterpenoids. The novelty of this review is the summary of these works, the analysis and identification of the most suitable biocatalysts for future researchers, and the presentation of development trends for new derivatives. The known biotransformation examples of the most common lupine-type triterpenoid … …OnLine Journal of Biological Sciences, Vol. 24, Issue 1 · 12 Dec 2023 · 4,948 accessesView PDFCite