10 results for “Suspension Culture”

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Research ArticleOpen accessPhrase in the titleSomatic Embryogenesis of Oil Palm (Elaeis guineensis Jacq.) from Bud Explants Using Suspension CultureNurul Huda Panggabean, Isnaini Nurwahyuni et al.The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability to produce entire plants without the need for gamete fusion. While somatic embryo proliferation is typically performed on solid media, this approach is less efficient than liquid media, which is both more cost-effective and can lead to better proliferation results. … …OnLine Journal of Biological Sciences, Vol. 23, Issue 2 · 4 May 2023 · 5,873 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,261 accessesView PDF Cite

Research ArticleOpen accessIn the abstractResponse of Root Growth and Root Border Cell Characteristics of Hemerocallis fulva to NaCl StressJinxiu He, Yongxu Qiao et al.Taking three Hemerocallis cultivars ('Jingzhouhua', 'Qingchong', 'Changzuizihua') with different salt tolerance as materials, the effects of 0-200 mmol/L NaCl on root morphology and Root Border Cell (RBC) characteristics were investigated using hydroponic and root suspension culture methods and one-way analysis of variance (ANOVA). The results showed that the root morphology indicators and activity of the high-salt-tolerant 'Jingzhouhua' decreased less than … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 27 Oct 2026 · 80 accessesView PDF Cite

Research ArticleOpen accessIn the abstractComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,919 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEffect of Elicitors and Precursors on the Synthesis of Anthocyanin in Grape Vitis vinifera Cell CulturesNay Min Min Thaw Saw, Heidi Riedel, Onur Kütük et al.Problem statement: Nowadays, plant cell suspension cultures and immobilized cells are being utilized for the higher yield and quality of the products than extraction of whole plants. Anthocyanins are compounds found in plants that have powerful antioxidant properties. They also provide some of the coloring or pigment of plants, flowers and fruits. Anthocyanin from grape cell cultures can be used … …Energy Research Journal, Vol. 1, Issue 2 · 31 Oct 2010 · 15,285 accessesView PDF Cite

Research ArticleOpen accessIn the abstractA Quick Method for Metarhizium anisopliae Isolation from Cultural SoilsM. A. Tajick Ghanbary, A. Asgharzadeh, A. R. Hadizadeh, M. Mohammadi SharifProblem statement: Fungi are one of the most active members in biological community of cultural soils. Many saprophyte and facultative parasitic fungi live in soil. Metarhizium anisopliae, one of the most famous soil inhabitant entomopathogens has a virulence potential on plant and animal pests. Approach: Introducing a new method for its isolation from soil was an applied method to find … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 2 · 30 Jun 2009 · 9,326 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDevelopment of Microbial Inoculants and the Impact of Soil Application on Rice Seedlings GrowthHayyan Ismaeil Al-Taweil, Mohammad Bin Osman, Aidil Abdul Hamid, Wan Mohtar Wan YussofProblem statement: The present study was undertaken to assess the Empty Fruit Bunches (EFB) as carrier of Biofertilizer and microbial inoculants interaction. A study in Malaysia had shown a strain of Trichoderma viride and Bacillus megaterium were isolated from the soil. Questions were raised whether the tow inoculants compatible to be mixed in same carrier? It is important to develop … …American Journal of Agricultural and Biological Sciences, Vol. 4, Issue 1 · 31 Mar 2009 · 8,722 accessesView PDF Cite

Research ArticleOpen accessIn the abstractAn Efficient Production of Oligosaccharides by a Reaction using Whole Mammalian Cells as BiocatalystsTomohisa Kato, Rie Mitsumori, Kenichi HatanakaProblem statement: Mammalian cells were used for the production of oligosaccharides by saccharide primer method. However, because cells in culture are used, productivity of oligosaccharides is low. Approach: In saccharide primer method employing cells under culture, glycosylation was performed only by mixing the collected cells in reaction mixtures. Saccharide primer, 12-azidododecyl β-lactoside that mimics lactosylceramide (LacCer), was incubated with various … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 4 · 31 Dec 2008 · 6,441 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDevelopment of Growth Medium for Centella Asiatica Cell Culture Via Response Surface MethodologyRozita Omar, M. A. Abdullah, M. A. Hasan, M. MarziahThe effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell … …American Journal of Applied Sciences, Vol. 1, Issue 3 · 30 Sep 2004 · 7,206 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBiological Control of Rice Blast (Magnaporthe oryzae) by use of Streptomyces sindeneusis isolate 263 in GreenhouseM. Ebrahimi Zarandi, G.H. Shahidi Bonjar, F. Padasht Dehkaei et al.Soil Actinomycetes particularly Streptomyces spp. have antagonistic activity against wide range of plant pathogens. In the recent decades they have attracted high interests as biocontrol agents. In search for finding such principles, in vitro suppression of Magnaporthe oryzae the causal agent of rice blast disease was studied by use of Streptomyces sindeneusis isolate 263 in greenhouse. Spray of rice seedling-leaves … …American Journal of Applied Sciences, Vol. 6, Issue 1 · 31 Jan 2009 · 11,496 accessesView PDF Cite