Sign inSubmit
Tips: “quotes” for a phrase · author:Lee · a DOI goes straight to its article

26 articles for “SDS-PAGE”
Set a search alert
Research ArticleOpen accessPhrase in the titleSDS-PAGE Protein Pattern and Antigenicity Cross Reaction of Human SchistosomesNahed A.A. EL-Ossily, Doaa A. Yones, Mohamed El-Salahy M.M. Monib et al.Schistosomiasis is one of the most important neglected tropical diseases. Its control depends on treatment with the available drug praziquantel. No vaccine exists despite the intense search for molecular candidates and adjuvant formulations over the last three decades. The present study aimed to compare the antigenic protein structures of Schistosoma mansoni, S. haematobium, Fasciola. hepatica and Echinococus granulosus hydatid cyst … …American Journal of Infectious Diseases, Vol. 12, Issue 1 · 10 Feb 2016 · 7,354 accessesView PDFCite
Review ArticleOpen accessIn the keywordsAssessing Protein PurityShiva Siddappa, Liang-Yin Ke, Chu-Huang Chen, Gopal Kedihithlu MaratheDetermination of protein purity is crucial, as proteins are frequently used as therapeutic agents and reagents in many biochemical reactions. Additionally, purified protein is necessary for biophysical and structural studies. Assessing protein purity involves examining for impurities or demonstrating their absence. In our experience, a satisfactory answer is seldom obtained when biochemistry students are asked a common question on how … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 4 · 9 Feb 2026 · 1,901 accessesView PDFCite
Research ArticleOpen accessIn the keywordsELISA-Based Development of Functional Antibodies for the Efficient Detection of Mycotoxigenic Fusarium (F. oxysporum and F. circinatum) and Diplodia (D. pinea) SpeciesPhumzile Mkhize, Phetole MangenaFusarium contains the most toxigenic and important fungal pathogens, producing a wide range of mycotoxins that include the more toxic secondary metabolites called fumonisins. This study analyzed the presence of common exo- and mycelium-soluble antigens, purified proteins using reducing SDS-PAGE and the expression of antibodies against different fungal pathogens. The results showed the presence of common antigens between pathogens and … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 4 · 18 Nov 2020 · 6,601 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGelatin Extraction of Alaska Pollock (Theragra chalcogramma) Dry SkinYanan Li, Fangyuan Chen, Jiong Zou et al.Alaska Pollock is rich in resource. And during its processing, skin is peeled as byproducts. The skin of Alaska Pollock contains lots of collagen or gelatin, promising materials, which can be used in food, cosmetics, biomedical and pharmaceutical industries. Extracting collagen from the dry skin of Alaska Pollock is studied in this study to provide a better basement for fully … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 28 Jan 2018 · 9,498 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Ferrous Ion Chelating Properties of Collagen Peptides from Dried and Fresh Cod SkinMengqian Chen, Cen Chen, Junchao Wu et al.Fish skin is a potential source of collagen and collagen is a protein source of bioactive peptides including iron chelating peptides. The aim of this study is to compare physicochemical properties of collagen extracted from fresh and dried cod skin and the ferrous ion chelating properties of collagen hydrolysates. Then the fresh and dried cod skin extracted collagen was hydrolyzed … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 3 · 2 Jul 2021 · 7,155 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 CellsShuqing Yang, Zhike Xie, Shuyan Yu et al.In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 26 Apr 2020 · 7,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Venom-Specific Fingerprints of Clinically Relevant Uruguayan Snakes by MALDI-TOFNorma Suarez, Victor MoraisSnakebite envenomation is a particular public health problem in many areas of the world, including extensive areas of Latin America. In particular, in Uruguay, snakebite accidents are caused by two snakes of the genus Bothrops: Bothrops alternatus and Bothrops pubescens. The only effective treatment available is the administration of the specific antivenom. Although there is an ELISA that allows the … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 26 Jul 2019 · 8,205 accessesView PDFCite
Research ArticleOpen accessIn the abstractHeterologous Expression and Biochemical Characterization of Lipase from Burkholderia cepacia Lu10-1Yao Zhang, Lu Wang, Yuanda SongEven though lipase genes from a variety of microorganism have been cloned and over-expressed, the prospective lipase resources for commercial production and industry application are still limited. In the present study, a lipase from Burkholderia cepacia Lu10-1 is heterologously over-expressed in Escherichia coli strain BL21(DE3) and purified to homogeneity. The molecular weight of the recombinant lipase from B. cepacia Lu10-1 … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Dec 2017 · 8,765 accessesView PDFCite
Research ArticleOpen accessIn the abstractIntracellular L-Asparaginase from Bacillus sp. PG02: Purification, Biochemical Characterization and Evaluation of Optimum pH and TemperatureFatemeh Izadpanah Qeshmi, Mahsa Rahimzadeh, Sedigheh Javadpour, Manijeh PoodatBacterial L-asparaginases are amidohydrolases that act on L-asparagine and produce L-aspartate and ammonia. These enzymes have been used in treatment of lymphoblastic leukemia. In the present study, a novel strain, Bacillus sp. PG02 was explored for the production of intra-cellular L-asparaginase enzyme. The nitrogen source for L-asparaginase production was L-asparagine. New intracellular L-asparaginase was purified using ion exchange chromatography and … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 1 · 26 Nov 2015 · 7,480 accessesView PDFCite