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35 results for “RFLP”
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Research ArticleOpen accessEvery word in the titleA NEW PCR-RFLP FOR SPECIES-SPECIFIC DIAGNOSIS OF SOUTH AMERICAN ANIMAL TRYPANOSOMIASISLiliana Torcoroma García, Yezid A. Ardila, Deilis Rincón et al.The diagnosis of animal trypanosomiasis in field samples is currently based on clinical suspicion and less frequently on traditional parasitological methods characterized by their very low sensitivity, especially in the sub-acute and chronic phase of the infection. Molecular tools such as Polymerase Chain Reaction have been shown to reach the highest sensitivity percentages, but without good resolution for all of … …American Journal of Animal and Veterinary Sciences, Vol. 9, Issue 2 · 5 Jun 2014 · 7,182 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleVibrio cholerae O1 Strains of Different Ribotypes have Similar hlyA RFLP Patterns but Different Vacuolating AbilityJorge E. Vidal, Paula Figueroa-Arredondo, Silvia Giono-Cerezo et al.Extensive cytoplasmic vacuolation on Vero and HeLa cells in vitro by the Vibrio cholerae pore forming toxin HlyA, has been previously reported by our group. Vibrio cholerae O1 and non-O1 pathogenic strains show differences in the potential to induce vacuolation, here we study occurring variations on vacuolating cytotoxic ability, related to changes in the nucleotide sequence of the hlyA-orf. A … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,984 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMarking of Meat Productivity Features in Pairs of bGH, bGHR and bIGF-1 Polymorphic Genes in Aberdeen-Angus CattleLaura Zhanedilovna Dushayeva, Askar Myrzahmetovich Nametov, Indira Saltanovna Beishova et al.The purpose of the study is to identify paired combinations associated with meat productivity in Aberdeen-Angus cattle for the genes of the somatotropin cascade (growth hormone bGH, growth hormone receptor bGHR, insulin-like growth factor-1 bIGF-1). The determination of animal genotypes was carried out by PCR-RFLP. Results of statistical processing was carried out using the software capabilities of "Microsoft Excel 2010" … …OnLine Journal of Biological Sciences, Vol. 21, Issue 2 · 22 Jun 2021 · 6,899 accessesView PDFCite
Research ArticleOpen accessIn the keywordsThe -863 TNF-α Polymorphism and Primary Open Angle Glaucoma in EgyptiansWalaa Bayoumie Abdulwahhab El Gazzar, Shaymaa Mohamed Abd El Rahman, Mohamed Nagy Elmohamady et al.Growing evidence indicates that TNF-alpha is involved in the pathogenesis of POAG in several ways, primarly by induction of retinal ganglion cells apoptosis and therefore optic nerve degeneration. TNF-alpha and POAG relationship has been studied at the genetic level with variable results in different populations. The transcription rate and the release of the TNF-alpha cytokine have been reported to be … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 2 · 1 Jun 2017 · 7,356 accessesView PDFCite
Research ArticleOpen accessIn the keywordsLeukemia Inhibitory Factor (LIF) Gene Polymorphism and its Impact on Reproductive Traits of PigsM. A. Leonova, L. V. Getmantseva, V. N. Vasilenko et al.The purpose of this study was to determine the frequencies of alleles and genotypes of the Leukemia Inhibitory Factor (LIF) gene (ID: 399503) in Landrace, Large White and Duroc pigs in Russia and to evaluate the impact on productive traits of pigs. Polymorphism was genotyped using PCR-RFLP method. In the course of studies we observed a different distribution of allele … …American Journal of Animal and Veterinary Sciences, Vol. 10, Issue 4 · 18 Oct 2015 · 7,404 accessesView PDFCite
Research ArticleOpen accessIn the keywordsINTERVENTION OF PHARMACOGENETIC PROFILING IN THERAPEUTICS OF KASHMIRI POPULATION, A LESSON LEARNED FROM CYP3A5*3 VARIANTAshaqullah Bhat, Aadil Wani, Rajinder Kaur et al.Human Cytochrome P450 3A, a variant of Cytochrome P-450- dependent monooxygenases is a major drug metabolizing enzyme. CYP3A has evolved to catalyze the biotransformation of its substrate. A great deal of inter-individual variation in expression levels exists for CYP3A5 gene. The common polymorphism in CYP3A5 that induces a cryptic splice site has been shown to cause a significant reduction in … …OnLine Journal of Biological Sciences, Vol. 14, Issue 3 · 10 Jul 2014 · 7,215 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCASE REPORT OF A PATIENT WITH RARE P.ARG136CYS (APOE2*) MUTATION IN APOLIPOPROTEIN E GENEJana Lisyova, Jan Chandoga, Stanislav Oravec et al.Apolipoprotein E (apoE) is a polymorphic glycoprotein associated with plasma lipoproteins and plays an essential role in the metabolism and clearance of plasma lipids. ApoE can represent the major genetic risk factor in development of premature atherosclerosis, coronary heart disease and late-onset familial and sporadic Alzheimer’s Disease (AD). We present a case report of a 51-year old male with suspected … …OnLine Journal of Biological Sciences, Vol. 14, Issue 1 · 9 Jan 2014 · 7,295 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCTLA-4 Gene Polymorphism in Relation to Thyroid Disorders in Patients with Chronic Hepatitis C Under Interferon Alpha TherapySalwa M. Abo El-Khair, Abd El-Hameed A. Metwali, Mohamed Abd El-Maksoud, Tarek BesheerThyroid autoimmunity and dysfunction have reported as side effects of interferon-α treatment. The CT60 and exon 1+49 A/G polymorphisms in the Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) gene have linked to susceptibility to autoimmune disease. The aim of this research was to analyze the frequencies of CTLA-4 CT60 and +49 A/G polymorphisms and evaluate both polymorphisms as indicators of thyroid disorder … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 3 · 20 Sep 2012 · 7,582 accessesView PDFCite
Research ArticleOpen accessIn the keywordsStudy and Identification of Insulin-Like Growth Factor-I Gene Polymorphisms in Zel Sheep PopulationSeyed Mohsen Kazemi, Cyrus Amirinia, Hossein Emrani, Shahaboddin GharahveysiProblem statement: The IGFs play an important role in regulating somatic growth according to nutritional conditions. Polymorphisms of IGF gene are reported to be significantly associated with many traits. Approach: In these study 142 samples of DNA from Zel sheep were used for detecting the polymorphisms of promoter region of the Insulin-like Growth Factor-1 (IGF-1) gene. Therefore DNA extracted from … …American Journal of Animal and Veterinary Sciences, Vol. 6, Issue 4 · 13 Jan 2012 · 7,739 accessesView PDFCite