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14 results for “Protein Purification”
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Research ArticleOpen accessPhrase in the titleEmerging Trends in Designing Short and Efficient Protein Purification ProtocolsSaurabh Gautam, Joyeeta Mukherjee, Ipsita Roy, Munishwar Nath GuptaProtein purification is carried out in both academic and industrial sectors. Both non-chromatographic approaches (precipitation, crystallization, partitioning) and chromatographic methods are used. When recombinant proteins are over expressed in foreign hosts, inclusion bodies are obtained. Many purification methods can be used to refold proteins. Many strategies allow one to avoid formation of inclusion bodies. The concept of affinity based separation … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 9,007 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDFCite
Research ArticleOpen accessIn the keywordsComparison of Radial and Axial Flow Chromatography for Monoclonal Antibody Downstream Processing at Bench and Pilot ScalesAli Demirci, Frank Leu, F. James BaileyAxial Flow Chromatography (AFC) is widely used for the purification of therapeutic Monoclonal Antibodies (MAbs). However, AFC columns can generate high pressure drops across the resin bed, preventing operation of the column at high flow rates especially at pilot or manufacturing-scales. Radial Flow Chromatography (RFC) was developed to provide lower pressure drops during chromatographic separations. In these studies, small and … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,508 accessesView PDFCite
Research ArticleOpen accessIn the keywordsMolecular Cloning and Heterologous Expression of Human Interferon Alpha2b GeneI. Made Artika, Yemima Budirahardja, Anna Lucia EkowatiHuman alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 4 · 20 Dec 2013 · 7,835 accessesView PDFCite
Research ArticleOpen accessIn the abstractInfluence of Tags on the Binding Affinity of Acyl-CoA Binding ProteinJizhong Han, Yunlong Sun, Yu Chen et al.Tagged fusion proteins are frequently employed for protein purification methods, but their effects on protein function and binding affinity are rarely studied. Here we expressed recombinant protein Acyl-CoA Binding Protein (ACBP) cloned from the full-length cDNA of Aspergillus oryzae and Saccharomyces cerevisiae. ACBP was expressed in Escherichia coli fused to a Maltose-Binding Protein (MBP) and Histidine-tag fusion. Recombinant ACBP was … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 23 Jan 2019 · 9,097 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Physical Characterization of Hydrophobin-Like Proteins (HLP) from Aerial Conidia of Metarhizium Anisopliae Var. AnisopliaeD. S.B. Kiong, Choon Fah J. Bong, P. J.H. KingThe aim of this study was to isolate and physically characterized Hyrophobin-Like Proteins (HLP) from the aerial conidia of two local isolates of Metarhizium anisopliae var. anisopliae, namely, TA and LR2. The protein samples were isolated based on their insolubility in hot Sodium Dodecyl Sulfate (SDS). The SDS-insoluble proteins were then purified in cold 98% formic acid and performic acid. … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 2 · 29 Jan 2015 · 7,776 accessesView PDFCite
EditorialOpen accessIn the abstractAre Heat Shock Proteins Druggable Candidates?Tawanda Zininga, Addmore ShonhaiBanumathy, G., V. Singh, S.R. Pavithra and U. Tatu, 2003. Heat shock protein 90 function is essential for Plasmodium falciparum growth in human erythrocytes. J. Biol. Chem., 278: 18336-18345. DOI: 10.1074/jbc.M211309200 Brehmer, D., S. Rudiger, C.S. Gassler, D. Klostermeier and L. Packschies et al., 2001. Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange. Nature Structural Molecular … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 4 · 2 Dec 2014 · 8,166 accessesView PDFCite
Research ArticleOpen accessIn the abstractCELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOSNicole Mika, Katharina Cierpka, Michaela C. Lange et al.As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Feb 2014 · 8,157 accessesView PDFCite
Research ArticleOpen accessIn the abstractDIFFERENT APPROACHES TO CRYSTALLIZATION OF MEMBRANE PROTEINSPrakash G. Doiphode, Vivek Pratap SinghCrystallography is more like an art than science. Crystallizing membrane proteins are a big challenge; membrane proteins are present in the cell membrane and serve as cell support. The most important feature of membrane protein is that it contains both hydrophobic and hydrophilic regions on its surface. They are generally much more difficult to study than soluble proteins. The problem … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 18 Dec 2012 · 7,288 accessesView PDFCite
Research ArticleOpen accessIn the abstractBioaugmentative Approaches for Dairy Wastewater TreatmentIrina Schneider, Yana TopalovaProblem statement: The achievement of a good ecological status of water receivers after discharge of waste or partially treated water from dairy industry requires harmonic interaction between water treatment technology and self-purification processes. Approach: The present research included two modules. First: an anaerobic treatment process for dairy wastewater in broadly spread sequencing batch bioreactor with fixed biomass was studied. As … …American Journal of Agricultural and Biological Sciences, Vol. 5, Issue 4 · 13 Dec 2010 · 8,894 accessesView PDFCite