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9 results for “Polar Body”
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Research ArticleOpen accessIn the keywordsAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDFCite
Research ArticleOpen accessIn the abstractInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDFCite
Review ArticleOpen accessIn the abstractChandrayaan-2: India’s Second Lunar Exploration MissionSantosh KosambeThe Moon has always been the center of attention for mankind, more than any other heavenly body in the night sky. With the early history of the solar system etched on it, the Moon challenges mankind from time immemorial to discover its secrets and admire its marvels. Understanding the Moon will provide us a pathway to unravel the early evolution … …Journal of Aircraft and Spacecraft Technology, Vol. 3, Issue 1 · 2 Nov 2019 · 9,542 accessesView PDFCite
Review ArticleOpen accessIn the abstractNasa Selects Concepts for a New Mission to Titan, the Moon of SaturnRelly Victoria Petrescu, Raffaella Aversa, Antonio Apicella, Florian Ion Tiberiu PetrescuNasa just has selected two new concepts for some new robotic missions, one of which aims to explore potential landing sites on Saturn’s largest moon, Titan. This first concept is named Dragonfly, which is a drone-like rotorcraft that would use the environment on Titan to fly to a number of locations to sample materials and determine surface composition to examine … …Journal of Aircraft and Spacecraft Technology, Vol. 2, Issue 1 · 19 Jan 2018 · 12,003 accessesView PDFCite
Research ArticleOpen accessIn the abstractStability and Ergonomic Design Features of Tractor Mounted Hydraulic Elevator for Coconut HarvestingKishor Purushottamrao Kolhe, Ashok G. Powar, Amit D. Dhakane, Sham H. MankarProblem statement: The most important design criterion of mechanized device was safety; safety comprises both the safety of operator and safety of maintenance personnel. Failures and safety of harvesting platform of Tractor Mounted Hydraulic Elevator (TMHE) powered by tractor PTO was tested by finite element method for the mechanical harvesting of coconut orchards using digital ergonomic hart rate meter. The … …American Journal of Engineering and Applied Sciences, Vol. 4, Issue 3 · 20 Sep 2011 · 8,326 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Changes of Some Electrolytes after Two Months ActivityM. J. Pourvaghar, A. R. ShahsavarSome investigators have speculated that sodium chloride losses in sweat associated with net dehydration may lead to the observed hyponatremia after prolong strenuous exercise. The effects of altered hydration can influence athletes cognitively in addition to physically. In this research, rest alterations of sodium and potassium of blood serum have been investigated. The subjects were male students who have been … …Journal of Social Sciences, Vol. 4, Issue 4 · 31 Dec 2008 · 7,935 accessesView PDFCite