14 results for “Oocytes”

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Research ArticleOpen accessEvery word in the titleAugmentation of Temperatures, Media and Cryoprotectants on in vitro Fertilization of Pig OocytesTumelo Thelma Maduwa, Masindi Lottus Mphaphathi et al.There are numerous challenges to the large-scale production of pig embryos using in vitro procedures. The aim of the present study was to compare the different holding temperatures, in vitro maturation media, and permeating cryoprotectants in the in vitro fertilization of pig oocytes. Ovaries were obtained from the local slaughterhouse, oocytes were retrieved by means of aspiration and slicing techniques … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 3 · 29 Aug 2024 · 4,650 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleSynergistic Effect of Insulin on in vitro Development of Immature Bovine OocytesMojtaba Dashtizad, Abd Wahid Haron, Rosnina Yusoff et al.Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 31 Dec 2010 · 10,433 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleEffect of Equilibration Temperature on In vitro Viability and Subsequent Embryo Development of Vitrified-Warmed Immature Bovine OocytesHajarian Hadi, H. Wahid, O. Abas Mazni et al.Problem statement: Vitrification is replacing conventional slow freezing to cryopreserve gametes and embryos especially for in vitro production of embryo in domestic animal species. However, the results are still not satisfactory. The aim of this experiment was to study the effect of different equilibration temperatures on in vitro viability of immature bovine oocytes after vitrification. Approach: Oocytes were obtained from … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 2 · 1 Sep 2010 · 16,116 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleIsolation and Culture of Oocytes from Dairy Cows' Ovaries for Productivity in the Southeastern Region of KazakhstanDauren Maratovich Bekenov, Batyrkhan Azimkhanovich Buralkhiev, Yusupzhan Artykovich Yuldashbayev et al.This research work aims to study methods for isolating and culturing follicular oocytes from Holstein cows in vitro. The proven complex of technologies for embryo transplantation on an industrial scale will provide a 3-5-fold acceleration of reproduction of highly productive cows. As a result of scientific research, biotechnological methods of accelerated reproduction of dairy cattle have been substantiated and introduced … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 14 Oct 2024 · 4,298 accessesView PDF Cite

Research ArticleOpen accessEvery word in the titleEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsAssessment of Chili Pepper (Capsicum annuum) as an Additive for the Prevention of CoccidiosisJenny Piedad Lozada-Ortiz, Oscar Patricio Núñez-Torres, Jorge Ricardo Guerrero-LópezNowadays, the use of plants is an alternative for the control and treatment of diseases in the poultry industry. The objective of this study was to evaluate the effect of chili pepper (Capsicum annuum) as a natural additive for the prevention of coccidiosis in broilers up to 8 weeks of age. For this experiment were used 288 Ross 308 chickens … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 27 Apr 2022 · 6,913 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsEffect of Fetal Serum and Follicular Liquor Supplementation on the In Vitro Production of Bovine EmbryosClara Larocca, Yael Filipiak, William Perez, Juan CalvoTo optimize In Vitro Fertilization (IVF) results in bovines by lowering costs, comparing the efficiency of Fetal Calf Serum (FCS) (high cost) with respect to bovine Follicular Fluid (bFF) (low cost) and by quantifying embryo production. Cumulus-Oocyte-Complexes (COC) obtained from a slaughter house, transported at 37°C in saline solution, were classified according to their quality in A, B, C and … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 9 Aug 2012 · 7,839 accessesView PDF Cite

Review ArticleOpen accessIn the abstractThe Impact of Di-2-Ethylhexyl Phthalate (DEHP) in Focus on the Reproductive System and Toxicity in Rat or Mammalian ModelVenkataramanaiah Poli, Srinivasulu Reddy MotireddyAbsalan, F., Saremy, S., Mansouri, E., Moghadam, M. T., Moghadam, A. R. E., & Razie, G. (2016). Effects of Mono-(2-Ethylhexyl) Phthalate and Di-(2-Ethylhexyl) Phthalate Administrations on Oocyte Meiotic Maturation, Apoptosis and Gene Quantification in Mouse Model. Cell Journal, 18(4), 503–513. https://doi.org/10.22074/cellj.2016.4717 Acevedo‐Rodriguez, A., Kauffman, A. S., Cherrington, B. D., Borges, C. S., Roepke, T. A., & Laconi, M. (2018). Emerging … …American Journal of Pharmacology and Toxicology, Vol. 19, Issue 1 · 6 Jun 2024 · 12,990 accessesView PDF Cite