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43 results for “Maturation”
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Research ArticleOpen accessEvery word in the titleComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInvestigation of Oocyte Retrieval Methods and Maturation Media on in vitro Development of Porcine EmbryosKayla M. Honneysett, Masindi L. Mphaphathi, Edward C. Webb, Ayanda MaqhashuThe objectives of the study were to: Compare oocyte retrieval methods (aspiration and slicing) from pig ovaries on oocyte quality and quantity; compare in vitro maturation (IVM) media (North Carolina State University -NCSU 37; Tissue Culture Medium-TCM 199; and porcine follicular fluid-mpFF) on oocytes polar body extrusion; compare the effect of Electrical Stimulation (ES) and frozen-thawed semen on cleavage rates … …American Journal of Animal and Veterinary Sciences, Vol. 16, Issue 4 · 5 Jan 2022 · 6,600 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleRal-Binding Protein is Required for the Maturation and Function of Dendritic CellsJozef Borvak, Mukesh Sahu, Varun Joy et al.Problem statement: Dendritic Cells (DCs) integrate responses of innate and adaptive immune system via transmitting pro-inflammatory and regulatory signals to naïve cells within draining lymph nodes. DCs have high capacity for endocytosis, both Clathrin-Dependent (CDE) and Caveolin-Dependent (CvDE). Approach: We have recently shown that ATP-hydrolysis by the multifunctional transporter protein RLIP76 is the primary determinant of the rate of CDE. … …American Journal of Immunology, Vol. 6, Issue 3 · 13 Sep 2010 · 8,095 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleInterleukin 10 Induces the Maturation of Putative Tolerogenic Monocyte-derived Dendritic Cells as Revealed by GeneArray AnalysisMarianne A. Jakobsen, Bjarne K. MØller, Klaus Rieneck et al.IL-10 is a multifunctional cytokine with known inhibitory effect on DC maturation. In the present study, we show that the presence of IL-10 during the first 6 days of maturation of Mo-DC has severe effects on the phenotype with IL-10 treated Mo-DC retaining a high expression of factors involved in antigen uptake and having low expression of costimulatory factors and … …American Journal of Immunology, Vol. 1, Issue 4 · 31 Dec 2005 · 6,621 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleMicronutrients, Hormesis and the Aptitude for the Maturation of RegulationJaap C. HanekampThe potential of hormesis as the default model to assess and manage chemicals is considered in relation to micronutrients. It is pointed out that micronutrients, despite their well-known U-shaped dose-response curve, are assessed and managed only with excess-exposure in mind. Hereby a schism between health and safety is unjustifiably realized. This proves to be the conundrum of basically all chemicals … …American Journal of Pharmacology and Toxicology, Vol. 3, Issue 1 · 31 Mar 2008 · 7,577 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEffect of Fetal Serum and Follicular Liquor Supplementation on the In Vitro Production of Bovine EmbryosClara Larocca, Yael Filipiak, William Perez, Juan CalvoTo optimize In Vitro Fertilization (IVF) results in bovines by lowering costs, comparing the efficiency of Fetal Calf Serum (FCS) (high cost) with respect to bovine Follicular Fluid (bFF) (low cost) and by quantifying embryo production. Cumulus-Oocyte-Complexes (COC) obtained from a slaughter house, transported at 37°C in saline solution, were classified according to their quality in A, B, C and … …American Journal of Animal and Veterinary Sciences, Vol. 7, Issue 3 · 9 Aug 2012 · 7,839 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSynergistic Effect of Insulin on in vitro Development of Immature Bovine OocytesMojtaba Dashtizad, Abd Wahid Haron, Rosnina Yusoff et al.Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes … …American Journal of Animal and Veterinary Sciences, Vol. 5, Issue 4 · 31 Dec 2010 · 10,433 accessesView PDFCite
Literature ReviewOpen accessIn the abstractMolecular Genetic Basis of Starch Biosynthesis and Waxy Allelic Variation in Rice Grain QualityInnabat Sartbayeva, Aigul Amirova, Dana Mynbaeva et al.The culinary, nutritional, and industrial properties of rice (Oryza sativa L.) grains are fundamentally governed by the structural arrangement of endosperm starch, a complex physiological phenotype shaped by genetic architecture and environmental conditions. This review provides a comprehensive synthesis of the molecular pathways driving starch biosynthesis in rice, centering on allelic diversity within the Waxy (Wx) locus, which codes for … …OnLine Journal of Biological Sciences, Vol. 26, Issue 3 · 22 Sep 2026 · 479 accessesView PDFCite