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8 results for “ISSR-PCR”
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Research ArticleOpen accessIn the keywordsDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessIn the keywordsGenetic State of Pulsatilla Patens (L.) Mill. Coenopopulations Based on DNA LabelingRustem Saltanovich Beishov, Gulnar Sultangazina, Indira Saltanovna Beishova et al.In the article, the results of studying the gene pools of the Kazakhstan coenopopulations of a rare plant species Pulsatilla patens (L.) Mill. are shown. The biomaterial taken from five coenopopulations located in Northern Kazakhstan has been used as samples. To identify the multilocus DNA polymorphism, the authors have used ISSR (Inter Simple Sequence Repeats, or intermicrosatellite analysis) labeling. The … …OnLine Journal of Biological Sciences, Vol. 21, Issue 1 · 2 Apr 2021 · 5,944 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Diversity of Common Wild Wheat Species in Armenia Using ISSR MarkersManvel Badalyan, Tatevik Aloyan, Varya Dilanyan et al.Armenia is one of the primary genealogical centers of numerous crops, the wild relatives of many of which are still found in Armenia today. Population limits of three out of 4 species of wild relatives of endemic wheat in Armenia (T. araraticum Jakubz, T. urartu Tumanian ex Gandilyan, T. boeoticum Boiss.) have been reduced in recent decades as a result … …OnLine Journal of Biological Sciences, Vol. 23, Issue 3 · 15 Aug 2023 · 5,217 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment, Polymorphism and Cross-Species Transferability of Genomic SSR Markers in Duabanga Moluccana, an Indigenous Tree Species from SarawakKit-Siong Liew, Wei-Seng Ho, Shek-Ling Pang, Julaihi AbdullahIn this study, we used ISSR-suppression methods to develop a set of SSR markers for Duabanga moluccana. It is an indigenous fast growing tropical tree species. A total of 44 SSR regions were identified and specific primer pairs were designed. The SSR motifs contained perfect compound with 24 (54.5%) occurrences, followed by the imperfect compounds with 8 (18.2%), simple perfect … …OnLine Journal of Biological Sciences, Vol. 16, Issue 1 · 26 Jan 2016 · 7,784 accessesView PDFCite
Research ArticleOpen accessIn the abstractCOMPARATIVE ANALYSIS OF INTER SIMPLE SEQUENCE REPEATS AND SIMPLE SEQUENCE REPEATS MARKERS: GENETIC ANALYSIS OF DESCHAMPSIA CESPITOSA POPULATIONS GROWING IN METAL CONTAMINATED REGIONS IN CANADAK.K. Nkongolo, S. Gervais, P. Michael, Y. ZhouComparative studies conducted on the genetic variation of metal-tolerant populations and their non-metal-tolerant counterparts have been performed on numerous species using isozyme markers. Analysis of genetic differences among plant populations growing in heavy metal-contaminated and uncontaminated regions are limited. The main objectives of the present study were to compare ISSR and microsatellite markers in assessing genetic variation in D. cespitosa … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 8 Apr 2014 · 7,286 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Opium Poppy (Papaver somniferum) GermplasmHari Shanker Acharya, Vimal SharmaProblem statement: The medicinal value of opium poppy (2n = 22, Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The genetic origin and diversity of cultivars used in the breeding collection is not fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated in the state to provide genetic information about heterogeneity, … …American Journal of Infectious Diseases, Vol. 5, Issue 2 · 30 Jun 2009 · 8,096 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Tospovirus Transmitting Thrips Populations from IndiaRang Lal Meena, T. Ramasubramanian, S. Venkatesan, S. MohankumarThe presence of Tospovirus in thrips vector, Scirtothrips dorsalis has been detected for the first time in India using RT- PCR analysis. Similarly RT-PCR analysis with GBNV infected tomato leaves also resulted in the amplification of cDNA corresponding to the N gene (approximately 830 bp) of Coimbatore isolate. Dendrogram constructed on the basis of RAPD similarity matrix revealed that S. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 6,733 accessesView PDFCite