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22 results for “Extracellular Production”

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Research ArticleOpen accessPhrase in the titleImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleChemical Mutagenesis for Improvement of Production of a Biologically Active Extracellular Polymeric Substance by Halomonas xianhensis SUR308Jhuma Biswas, Amal K. PaulA moderately halophilic bacterium, Halomonas xianhensis SUR308 (Genbank Accession No. KJ933394) isolated from solar saltern of Surala, Odisha, India was found to produce significant amount of a biologically active extracellular polymeric substances (EPS). Under optimized cultural conditions, the strain was able to produce 7.05 g L-1 of EPS after 8 days of incubation under shake culture. To enhance the EPS … …Current Research in Microbiology, Vol. 7, Issue 1 · 1 Apr 2016 · 15,918 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Production of Extracellular Alkaline Lipase by an Improved Strain of Pseudomonas aeruginosa MTCC 10,055Deepali Bisht, Santosh Kumar Yadav, Nandan Singh DarmwalProblem statement: Lipases are industrially important enzymes having applications in numerous industries. For easy commercialization it is necessary to produce lipases at industrial level which could be achieved by strain improvement and medium formulation. Approach: In the present study strain improvement of Pseudomonas aeruginosa MTCC 10,055 was done by chemical mutagenesis using mutagen 4-nitroquinoline1-oxide for alkaline lipase production. Different fermentation … …American Journal of Applied Sciences, Vol. 9, Issue 2 · 24 Nov 2011 · 8,760 accessesView PDFCite
Research ArticleOpen accessIn the keywordsProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessIn the abstractCross Talk between Iron Regulatory Proteins and Proinflammatory Molecules in Ovarian Cancer Patients Based on Menopausal StatusHaritha Padannapurath Manoj, Sudha Kuthethur, Reshma Kumarchandra et al.Micronutrient iron is postulated to contribute to the initiation of cancer mainly by the formation of Reactive Oxygen Species (ROS), the key signaling molecules of inflammatory reactions. Iron may also cause elevation of MMP9, a gelatinase responsible for remodeling extracellular matrix in cancer cells through the ROS pathway. Estrogen the hormone which decreases markedly in post menopause is also known … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 31 May 2024 · 4,491 accessesView PDFCite
Research ArticleOpen accessIn the abstractProtective Role of Ganoderma lucidum Polysaccharides Against Stress Induced by Heavy Metals in Caenorhabditis elegansHuixiu Ma, Dehui Dai, Guicai Chen, Weilian HuAli, H., Khan, E., & Ilahi, I. (2019). Environmental Chemistry and Ecotoxicology of Hazardous Heavy Metals: Environmental Persistence, Toxicity, and Bioaccumulation. Journal of Chemistry, 2019, 6730305. https://doi.org/10.1155/2019/6730305 Aljerf, L., & Aljerf, N. (2023). Food products quality and nutrition in relation to public. Balancing health and disease. Progress in Nutrition, 25(1), e2023024. https://doi.org/10.23751/pn.v25i1.13928 Aljerf, L., Williams, M., Ajong, A. B., Onydinma, … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 2 · 18 May 2024 · 6,947 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiogenic Silver Nanoparticles Produced by Bacteria Isolated from University Campus Environment Soil Samples in Ras Al KhaimahPooja Shivappa, Grisilda Vidya Bernhardt, Sheela Haridas, Michael V MagaogaoSilver is observed to be comprised of a huge percentage ofsilver oxide and the utilization of silver and silver salts is in practicesince ancient human civilization. Silver nanoparticles remain to be potentialantifungal, antibacterial, anti-inflammatory, and antiviral agents. Further, itis demonstrated that silver nanoparticles have been involved in arresting thegrowth of several bacterial species thereby reducing their harmful effects.Micro-organisms are being … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 5 Sep 2022 · 5,870 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces SpeciesFerdinand U. Paschaline, Onyeocha O. Ignatius, Ezeji E. Uchechukwu et al.The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarbon polluted soil and identified using standard microbiological and biochemical techniques. Fermentation was carried out for enzyme production and the extracellular extract was precipitated with 50% ammonium sulphate salts and further purified by gel filtrationusing sephadex … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 7 Dec 2019 · 7,398 accessesView PDFCite
Review ArticleOpen accessIn the abstractPlant Growth Promoting Rhizobacteria (PGPR): A Novel Agent for Sustainable Food ProductionOdoh Chuks Kenneth, Eze Chibuzor Nwadibe, Akpi Uchenna Kalu, Unah Victor UnahPlant Growth Promoting Rhizobacteria (PGPR) involves the use of large array of soil bacteria to improve yield, plant growth and sustainable food production. As free living and symbiotic rhizobacteria, PGPR exert its role by colonizing extracellular and/or intracellular rhizoenvironment in the quest for carbon source. In the past decades, focus has been on developing a biosafety agro base approach void … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Apr 2018 · 14,183 accessesView PDFCite