19 results for “Enzyme Stability”

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Research ArticleOpen accessPhrase in the titleEnhanced Amylase Activity by Modulating Abiotic Factors and Enzyme Stability in the Thermophilic Bacterial IsolatesAnthoni Agustien, Denny Bendrianis, Feskaharny Alamsyah et al.Amylase is one of the enzymes that is widely used in bioprocess technology, amylase from different microbial sources also varies in form, so it is suitable for its specific application. The purpose of this study was to determine the optimum temperature, pH, agitation, and stability of the amylase enzyme from two thermophilic bacterial isolates, TUA-07 and TUA-09. This study used … …OnLine Journal of Biological Sciences, Vol. 24, Issue 2 · 7 Feb 2024 · 5,493 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsThe Effect of NaCl on the Stability of Ab3 Lipase from Sphingobacterium sp. that Co-Expressed with LEA K Using In silico ApproachIbrahim Muhammad et al.This study explores how sodium chloride (NaCl) influences the binding affinity and stability of the Ab3 lipase-LEA K complex when interacting with olive oil, using molecular docking techniques. The docking simulations, conducted with AutoDock Vina, reveal that the presence of NaCl significantly enhances the binding affinity of the enzyme-substrate complex, increasing it from-6.55 kcal/mol in the absence of salt to-7.81 … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 9 Mar 2025 · 5,599 accessesView PDF Cite

Research ArticleOpen accessIn the keywordsPotential Applications of Chitosan Nanoparticles as Novel Support in Enzyme ImmobilizationHoda Jafarizadeh Malmiri, Mohammad Ali Ghaz Jahanian, Aydin BerenjianChitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,302 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPotentiometric Biosensor for Highly Toxic Pb (lead) and Cd (cadmium) Detection in Milk Using Layer-by-Layer ImmobilizationZhainagul Kakimova, Klara Zharykbasova, Aitbek Kakimov et al.Milk contamination with heavy metals such as lead (Pb) and Cadmium (Cd) poses a serious threat to public health, particularly in industrial regions. To address the need for rapid and on-site detection of these contaminants, a potentiometric biosensor was developed for the quantification of Pb²⁺ and Cd²⁺ ions in milk and dairy products. The biosensor functions by measuring the change … …OnLine Journal of Biological Sciences, Vol. 25, Issue 4 · 8 Feb 2026 · 1,480 accessesView PDF Cite

Research ArticleOpen accessIn the abstractDiscovery of a New Thermostable Chitinase from Streptomyces No. 6Xiaowen Que, Yonghong Bao, Qing Liu et al.Chitinases are a class of hydrolases that hydrolyze the β-1, 4-glycosidic bonds between N-acetyl-D-glucosamine (GlcNAc) in chitin. Among chitinases, thermophilic chitinases and thermostable chitinases have received extensive attention in recent years due to their ability to tolerate higher temperatures and maintain enzyme stability for a longer period of time. However, it has previously been observed that not all chitinases are … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 3 · 1 Aug 2024 · 5,115 accessesView PDF Cite

Research ArticleOpen accessIn the abstractOptimization and Molecular Identification of Protease-Producing Thermophilic Bacterial Isolate TUA-26Anthoni Agustien, Mufidhatul Muqarramah, Feskaharny AlamsjahProtease enzymes are used as biocatalysts that can accelerate a reaction. Thermostable enzymes produced by thermophilic bacteria are needed in the industrial field because of their ability to withstand high temperatures. The thermophilic bacterial isolate TUA-26 that produces protease was to analyze the optimum conditions of temperature, pH, agitation, trace elements, carbon sources, and nitrogen sources; the stability of protease … …OnLine Journal of Biological Sciences, Vol. 24, Issue 3 · 6 Apr 2024 · 4,935 accessesView PDF Cite

Research ArticleOpen accessIn the abstractInvestigation of the Contribution of Late Embryogenesis Abundant (LEA) K Peptide in Enhancing the Expression of Lipase from Sphingobacterium sp.: In vitro and in Silico StudiesIbrahim Muhammad et al.Sphingobacterium sp. AB3 lipase is a psychrophilic enzyme with optimal lipolytic activity at pH 7 and 15°C. The cold-adapted properties of the lipase render it suitable for various low-temperature industrial applications such as biodiesel production, detergent formulation, and non-thermal food processing. Recent studies have shown that co-expression of LEA K peptide with AB3 lipase resulted in enhanced protein expression in … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 21 Nov 2024 · 4,569 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProduction and Characterization of Hydrocarbon Degrading Peroxidase from Rhizopus and Saccharomyces SpeciesFerdinand U. Paschaline, Onyeocha O. Ignatius, Ezeji E. Uchechukwu et al.The production, characterization and partial purification of hydrocarbon degrading peroxidase from di-culture of Rhizopus and Saccharomyces species was assessed. The fungi were isolated from hydrocarbon polluted soil and identified using standard microbiological and biochemical techniques. Fermentation was carried out for enzyme production and the extracellular extract was precipitated with 50% ammonium sulphate salts and further purified by gel filtrationusing sephadex … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 4 · 7 Dec 2019 · 7,398 accessesView PDF Cite

Research ArticleOpen accessIn the abstractProduction, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulansLuis Lascano-Demera, Patricia Portero Barahona, Enrique Javier Carvajal BarrigaThe production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 11 Apr 2019 · 10,020 accessesView PDF Cite

Research ArticleOpen accessIn the abstractHide Dehairing and Laundry Detergent Compatibility Testing of Thermostable and Solvents Tolerant Alkaline Protease from Hot Spring Isolate Bacillus cohniiU3Pravin R. Dudhgara, Bhavsar Sunil, Ghelani AnjanaIn the present study, alkaline protease producing thermophilic Bacillus cohniiU3 strain was isolation from Unnai hot spring, India. The maximum production of protease (344 U/mL) was reported after 72 h of inoculation at 50°C in shake flask culture using the gelatin casein medium. The protease was found to remain active up to 96 h and production was growth dependent. The … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 23 Jul 2015 · 7,335 accessesView PDF Cite