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31 results for “Electrophoresis Gel”
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Research ArticleOpen accessEvery word in the titleSwarm Optimization Techniques for Segmenting Gel Electrophoresis ImagesSara Ibrahim Ibrahim, Mohamed Abd Allah Makhlouf, Ghada.S. El-Tawel, M.E. WahedGel Electrophoresis (GE) are discussed as the main tool to dissociate DNA sequences. It helps in analyzing the genome such that each image resulting from it consists of lanes that include several bands. Image segmentation plays the foremost role in image processing. It helps in producing accurate results in medical diagnosis. Image segmentation works by dividing an image into regions … …Current Research in Bioinformatics, Vol. 5, Issue 1 · 18 Jun 2016 · 8,092 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleA Recursive Application of a Support Vector Machine for Protein Spot Detection in 2-Dimensional Gel ElectrophoresisGary D. Boetticher, Hisham Al-Mubaid, Karen Frasier-ScottTwo-dimensional polyacrylamide gel electrophoresis (2-D PAGE) analysis remains the core of proteomic technology because it is currently the most powerful method to analyze large collections of proteins. Advances in electrophoresis equipment are making this technique more accessible but effective computer assisted protein spot detection remains a very labor-intensive endeavor. Protein spot analysis is still time consuming, requires human intervention and … …Journal of Computer Science, Vol. 1, Issue 3 · 30 Sep 2005 · 6,675 accessesView PDFCite
Research ArticleOpen accessEvery word in the title2-D Gel Electrophoresis Map of Methicillin-Resistant Staphylococcus aureus Treated with Quercus Infectoria Gall ExtractDayang Fredalina Basri, Lee Seng Aik, Radhiah Khairon, Mariati Abdul RahmanThe widespread outbreak of Methicillin-Resistant Staphylococcus Aureus (MRSA) has caused clinical and epidemiological concern in hospital environment. The emergence of Vancomycin-Intermediate S. Aureus (VISA) and, more recently, Vancomycin-Resistant S. Aureus (VRSA) has further alarmed clinician and scientist worlwide. The objective of this study is to determine the optimum concentration of sample protein from MRSA after treatment with acetone extract from … …American Journal of Biochemistry and Biotechnology, Vol. 9, Issue 1 · 18 Mar 2013 · 7,994 accessesView PDFCite
Research ArticleOpen accessIn the keywordsProteomics Approach for Investigating the Disease Resistance Using Cucumber as Model PlantSaligrama A. Deepak, Junko Shibato, Hideo Ishii et al.The profile of proteins induced in cucumber leaves pretreated with Acibenzolar-S-Methyl (ASM) and challenge inoculated with Colletotrichum orbiculare are presented. ASM was applied to the first leaf and the third leaf collected at different time intervals of 0-48 h and the total protein extracted using trichloroacetic acid acetone was analyzed by two-dimensional gel electrophoresis. Increased expressions of pyridoxine biosynthesis protein, … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 7,240 accessesView PDFCite
Research ArticleOpen accessIn the keywordsHeroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA)Jessica L. Reynolds, Supriya D. Mahajan, Donald Sykes, Madhavan P.N. NairHeroin use is postulated to act as a cofactor in the neuropathogenesis of human immunodeficiency virus (HIV-1) infection. Astrocytes, integral components of the CNS, are reported to be susceptible to HIV-1 infection. Upon activation, astrocytes release a number of immunoregulatory products or modulate the expression of a number of proteins that foster the immunopathogenesis of HIV-1 infection. However, the role … …American Journal of Infectious Diseases, Vol. 2, Issue 2 · 30 Jun 2006 · 7,261 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,724 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA Proteomics Analysis of Drought Stress-Responsive Proteins as Biomarker for Drought-Tolerant Sugarcane CultivarsNisachon Jangpromma, Supansa Kitthaisong, Khomsorn Lomthaisong et al.Problem statement: The prime objective in breeding selection process of drought-tolerant sugarcane is to identify the correlating marker, which could lead to rapid screening for drought-tolerant cultivars. In this study, we have reported an unknown 18-kDa protein (p18) along with other stress-inducible proteins to be highly expressed in sugarcane leaves under drought stress condition. Approach: The 2D-PAGE patterns of proteins … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 2 · 30 Jun 2010 · 9,814 accessesView PDFCite
Research ArticleOpen accessIn the keywordsAlterations of HDL Subclasses in Different Lipid Levels of Men and WomenYuye Yang, Luchuan Yang, Mingde Fu et al.With the increase of plasma TG level, both in men and women, apoA-I concentrations of preβ1-HDL and HDL3a were significantly higher while HDL2a and HDL2b were significantly lower. Similarly, with the increase of plasma LDL-C level, apoA-Iconcentrations of preβ1-HDL, HDL3c(in men) and HDL3b were significantly higher while apoA-Iconcentrations of HDL2b were significantly lower. And with the decrease of plasma HDL-C, … …American Journal of Immunology, Vol. 1, Issue 2 · 30 Jun 2005 · 9,075 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain EnzymeRafika Mila Amanah, Dita Prameswari Trenggono Putri, Mohammad Zainal Abidin et al.The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium … …American Journal of Animal and Veterinary Sciences, Vol. 20, Issue 2 · 24 May 2025 · 5,165 accessesView PDFCite
Research ArticleOpen accessIn the abstractCharacteristics of Collagen from Parrotfish (Chlorurus Sordidus), Tiger Grouper (Epinephelus Fuscoguttatus) and Pink Ear Emperor (Lethrinus Lentjan): Effect of Acetic Acid Concentration and Extraction TimeAsep Awaludin Prihanto, Abdul Aziz Jaziri, Mohammad D. Pratomo et al.The use of marine fish's skin waste such as parrotfish (Chlorurus sordidus), tiger grouper (Epinephelus fuscoguttatus), and pink ear emperor (Lethrinus lentjan) needs improvement to increase its economic value and sustainability. Therefore, this study aims to investigate the effect of fish skin to acetic acid ratio with different extraction times on the yield and characteristics of skin collagen obtained from … …OnLine Journal of Biological Sciences, Vol. 22, Issue 1 · 28 Jan 2022 · 6,675 accessesView PDFCite