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240 articles for “E.coli”
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Research ArticleOpen accessEvery word in the titleExpression and Induction Optimization of Mucor Circinelloides Lipase in Escherichia ColiYueping Yang, Yao Zhang et al.Microbial lipases serve as versatile biocatalysts for modifying lipid substrates, enabling diverse biotechnological applications spanning food technology, renewable energy production, and pharmaceutical sectors. Genomic analysis of Mucor circinelloides WJ11 revealed multiple lipase-encoding genes, with WJ_23 successfully cloned and heterologously expressed in Escherichia coli. Since the lipase expression was mainly concentrated in the cell pellets, appropriate induction strategies had been investigated … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 2 · 13 Jun 2025 · 5,982 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExtraction Optimization of Essential Oil from Medicago hispida and Preliminary Inhibitory Mechanism Against Escherichia coliXiulan Chu, Qianfeng Chen, Tong Wang et al.In this study, the extraction optimization, characterization, antioxidant evaluation and preliminary inhibitory mechanism against Escherichia coli of the essential oil from dried Medicago hispida (EOMH) were explored to uncover the pending questions that had been raised in our previous work and to enlarge the industrial potential of EOMH production. The optimal parameters for EOMH extraction were: Extraction time of 7 … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 20 Nov 2021 · 5,789 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleOccurrence of Antibiotic Resistant C. jejuni and E. coli in Wild Birds, Chickens, Environment and Humans from Orang Asli Villages in Sungai Siput, Perak, MalaysiaIbrahim Mohamed Mohamed-Yousif, Jalila Abu, Saleha Abdul-Aziz et al.In developing countries, Escherichia coli and Campylobacter jejuni were found to be the prominent bacterial causes of gastroenteritis in human gastroenteritis, and they also showed an increased trend towards antibiotic resistance. Thus, the study aimed to determine the occurrence of E. coli and C. jejuni in wild birds, chickens, human and environment in villages in Perak and to determine the … …American Journal of Animal and Veterinary Sciences, Vol. 14, Issue 3 · 5 Aug 2019 · 8,909 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleProduction of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coliDessy Natalia, Christian Heryakusuma, Fernita Puspasari et al.Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 10 Oct 2018 · 10,346 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleImprovement on Extracellular Production of Recombinant Burkholderia cepacia Lu10-1 Lipase by Escherichia coliYao Zhang, Yuanda Song, Lu Wang, Qing LiuThe purpose of this study was to investigate several strategies on enhancing extracellular production of recombinant lipase from Burkholderia cepacia Lu10-1 in recombinant Escherichia coli BL21(DE3). In the present study, a fed-batch fermentation strategy for the excellular production of lipase by E. coli has been established. First of all, different induction methods (including selection of inducers, inducer concentration, induction temperature … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 30 May 2018 · 11,539 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleEnhanced Fatty Acid Production in Escherichia coli by Over-Expression of NADPH Generating EnzymesYuanda Song, Yong Q. Chen, Haiqin Chen et al.NADPH is an essential factor for Fatty Acid (FA) biosynthesis. The effect of NADPH generating enzymes [NADP+-dependent malic enzyme (NADP-ME), NADP+-dependent isocitrate dehydrogenase (NADP-IDH), Glucose 6-Phosphate Dehydrogenase (GPD) and 6-Phosphogluconate Dehydrogenase (PGD)] on FA biosynthesis was investigated in an engineered Escherichia coli BL21 △fadE/pTE. Among NADPH-generating enzymes, GPD and ICD increased total FA production by 60 and 16% respectively. Especially … …American Journal of Biochemistry and Biotechnology, Vol. 13, Issue 4 · 23 Oct 2017 · 8,578 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAntimicrobial Susceptibility of Community Acquired Escherichia coli in Urinary Tract Infections (UTI) in Benin for Eleven Years (2005-2015)Tchiakpe Edmond, Laurence Carine Yehouenou, Zahra Fall Malick et al.Antimicrobial resistance became a growing public health problem in the world and Escherichia coli (E. coli) appeared as one of nine bacteria commonly causing infections in community and hospitals. It prevalence and it resistance to antibiotics were evaluated in Benin throughout an eleven year period. In this retrospective study, routine urine samples from patients collected at the National Laboratory (NL) … …American Journal of Infectious Diseases, Vol. 13, Issue 2 · 2 Oct 2017 · 7,775 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleResistance in Escherichia coli Strains Isolated from Pig Faecal Samples and Pig farm Workers, GreeceGeorge Valiakos, Alexandros Vontas, Constantina N. Tsokana et al.In this study, we compare the degree of antibiotic resistance between the E. coli strains isolated from swine faecal and farm workers. Resistance in nine widely used antibiotics was assessed by the determination of Minimum Inhibitory Concentration. Sixty-four out of 72 E. coli isolates derived from pigs and sixty out of 72 derived from farm workers showed resistance to at … …American Journal of Animal and Veterinary Sciences, Vol. 11, Issue 4 · 5 Nov 2016 · 7,505 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleExtended-Spectrum β-Lactamases Producing Escherichia coli Strains Monitored Over 4 Years in The University Hospital in Košice, SlovakiaViera Lovayová, Lucia Vargová, Mária Nagyová et al.The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. The presented study provides identification and determination of the spectrum of resistance against different and clinically used antimicrobial drugs in the clinical isolates of Escherichia coli. These isolates had their origin in different departments of the University Hospital L. Pasteur in Košice. … …Current Research in Microbiology, Vol. 7, Issue 1 · 16 Jun 2016 · 14,537 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleCorrelation Between in vitro Biofilm Formation and Virulence Properties of Extra-Intestinal Pathogenic Escherichia Coli (Expec)Md. Fakruddin, Khanjada Shahnewaj Bin Mannan, Reaz Mohammad MazumdarAbout 65 Escherichia coli strains isolated from extra-intestinal sites were included in this study to determine their biofilm formation capability and virulence properties. About 87% ExPEC isolates were motile and 12.3% were found to be non-motile. Only 12.30% isolates were strong biofilm former (SBF >1.5) whereas 18.46% isolates were non biofilm former. About 71% isolates were capable to express curli … …OnLine Journal of Biological Sciences, Vol. 14, Issue 4 · 14 Jan 2015 · 6,942 accessesView PDFCite