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88 articles for “Culture Medium”

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Research ArticleOpen accessPhrase in the titleEvaluation of Antioxidant and Antibacterial Activities of the Polysaccharides from Mycelium and the Culture Medium of Pleurotus Eryngii Strain UN-1Qing Liu, Haodong Wu, Ying Wu et al.Pleurotus eryngii was one of the most precious and ordinary edible mushrooms, and its polysaccharides were the major active substances. In this study, P. eryngii strain UN-1 was cultured in a liquid medium at 25℃ under a shaking of 80 rpm for 7 days. Two crude polysaccharides named CPN and CPNM were obtained from the mycelium of P. eryngii UN-1 … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 3 · 19 Jul 2022 · 6,176 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleProduction of Selenium-Enriched Yeast (Kluyveromyces marxianus) Biomass in a whey-based Culture MediumNicolás Gurdo, Mario Calafat, Diego Gabriel Noseda, Isabel GigliTwo important aspects of agriculture intensification are the reduction in the concentration of specific soil minerals that affects livestock production and the increase of agricultural by-products, which produce environmental pollution. In this regard, whey - a cheese by-product-often is considered a wasted-product. Due to its lactose concentration, (4.5%), when whey is discarded without treatment generates a high Biological Oxygen Demand … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 28 Jun 2018 · 11,375 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleDevelopment of Growth Medium for Centella Asiatica Cell Culture Via Response Surface MethodologyRozita Omar, M. A. Abdullah, M. A. Hasan, M. MarziahThe effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell … …American Journal of Applied Sciences, Vol. 1, Issue 3 · 30 Sep 2004 · 7,204 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBasal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic EmbryogenesisJameel M. Al-KhayriProblem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 10,156 accessesView PDFCite
InvestigationOpen accessIn the keywordsEfficiency of Microtubers Application in the Production of Original Potato SeedsZhursinkul Abdugapparovna Tokbergenova, Sailau Akhmetovich Babayev, Dauriya Urazgaliyevna Togayeva et al.The development of the technology of mass microtubers cultivation in vitro based on innovative methods with respect to potato varieties allowed for use in Kazakhstan has an important theoretical and practical significance for the development of seed production in the Republic. The goal is to develop a technology for mass cultivation of potatoes microtubers in vitro based on biotechnology methods … …OnLine Journal of Biological Sciences, Vol. 17, Issue 4 · 6 Nov 2017 · 7,402 accessesView PDFCite
Research ArticleOpen accessIn the keywordsModeling Growth of Microalgae Dunaliella Salina under Different Nutritional ConditionsSergio Santos Jesus, Rubens Maciel FilhoProblem statement: The aim of this study was to find the empirical model that describes the growth kinetics of Dunaliella salina, with low production cost and to estimate parameters of this model. Approach: In this study the strain of D. salina UTEX 200 was cultivated in seawater (0.5 M NaCl) at room temperature with agitation of 150 rpm and luminous … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,014 accessesView PDFCite
Research ArticleOpen accessIn the abstractMarine-Derived Extracts as Economically Sustainable and Ethical Alternatives to Fetal Bovine Serum in HEK293 Cell CultureKian Kenneth Francisco Sanchez, Jennifer Seki-Wong et al.Fetal Bovine Serum (FBS) is a staple supplement in cell culture, providing key growth factors that promote cell health and proliferation. Since 1885, cell culture has been a cornerstone of both basic and applied research, leading to significant discoveries of cell communication and function. However, the use of FBS presents economic and ethical challenges. Millions of bovine fetuses are euthanized … …American Journal of Biochemistry and Biotechnology, Vol. 22, Issue 3 · 7 Aug 2026 · 1,042 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Analysis of Surface Microhardness and Antimicrobial Activity of Conventional and Bioactive Composite Materials for Dental RestorationsBruno Fongaro Dalbosco, Rafael da Silva Vanolli, Poliana Maria de Faveri Cardoso et al.This study evaluated the fluoride release capability, antimicrobial activity, and surface microhardness of bioactive composite resins, critical properties for the effectiveness and durability of dental materials in restorative procedures. Conventional and bioactive composite resins with varying viscosities were tested. Specimens of each resin type were prepared and stored in physiological saline solution for 24 hours to simulate oral conditions. Surface … …Current Research in Dentistry, Vol. 16, Issue 1 · 29 Jan 2026 · 2,809 accessesView PDFCite
Research ArticleOpen accessIn the abstractAnalysis of the Utility of MPT64, Drug Susceptibility Testing and Tuberculosis/Nontuberculous Mycobacteria Reverse Transcription Polymerase Chain Reaction for Tuberculosis Diagnosis and Efficiency Compared with Traditional CultureHo Keun Choi, Ga Yeon Kim, Tae Soung Kim, Jae Kyung KimDefining the molecular mechanisms underlying the dormant state of Mycobacterium tuberculosis (MTB) is essential for developing more effective treatments for tuberculosis (TB) infection. We investigated the positivity and resistance rates of TB tests on 6,174 samples referred to a single hospital. Of these, 342 cases (5.5%) were confirmed as positive by acid-fast bacilli staining, 243 cases (71.1%) were detected as … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 4 · 5 Dec 2024 · 4,206 accessesView PDFCite
Research ArticleOpen accessIn the abstractIsolation and Culture of Oocytes from Dairy Cows' Ovaries for Productivity in the Southeastern Region of KazakhstanDauren Maratovich Bekenov, Batyrkhan Azimkhanovich Buralkhiev, Yusupzhan Artykovich Yuldashbayev et al.This research work aims to study methods for isolating and culturing follicular oocytes from Holstein cows in vitro. The proven complex of technologies for embryo transplantation on an industrial scale will provide a 3-5-fold acceleration of reproduction of highly productive cows. As a result of scientific research, biotechnological methods of accelerated reproduction of dairy cattle have been substantiated and introduced … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 14 Oct 2024 · 4,298 accessesView PDFCite