6 results for “Cell Immobilization”

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Research ArticleOpen accessIn the keywordsImmobilized Whole Cell System with Methyl Parathion-Degrading Activity for Organophosphate Insecticide DetectionChadaporn Pootawee, Witsanu Senbua, Jesdawan WichitwechkarnAs the result of the extensive use and high contamination of organophosphate (OP) insecticides in agricultural products and the environment, especially in developing countries, a simple and convenient means for OP detection and screening was investigated. Whole cells from the recombinant clone BpGP carrying methyl parathion (MP) degrading, mpdB, gene originating from MP-degrading Burkholderia cepacia and possessing MP-degrading activity were … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 18 Sep 2018 · 7,673 accessesView PDF Cite

Research ArticleOpen accessIn the abstractYeast Immobilization on Peanut Hulls for Potential Application in Beer FermentationWaldir D. Estela-Escalante, Sergio Rosales-Mendoza, Mario Moscosa-SantillánThe potential of using peanut hulls as support material for immobilization of yeast cells during beer fermentation was evaluated. After treatment of peanut hulls with 0.5% (w/v) NaOH, FTIR analysis revealed an important change on its chemical composition. For instance, the proportion of lignin decreased and as a result the adsorption of yeast cells onto the support material during fermentation … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 3 · 22 Jul 2019 · 8,375 accessesView PDF Cite

Research ArticleOpen accessIn the abstractStatistical Improvement of Batch Culture with Immobilized Pichia pastoris Cells for rPOXA 1B Laccase ProductionMichelle Gouzy-Olmos, Luis M. Cháves-Tequia, María F. Rojas-Fajardo et al.Immobilized Pichia pastoris X33/pGAPZαA-LaccPost-Stop in Ca2+ alginate beads was employed for Pleurotus ostreatus rPOXA 1B laccase batch production. Sequential statistical improvement was achieved through Plackett-Burman (PBED), (PBED-T11, 29.5±0.8 UL-1), which allowed to increase activity by 2.36-fold (12.5±2.6 UL-1) obtained in a preliminary study. Following, Box-Behnken Experimental Design (BBED) was implemented and obtained enzymatic activity in PBED-T11 was further increased by … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 26 Apr 2018 · 11,776 accessesView PDF Cite

Research ArticleOpen accessIn the abstractOPTIMIZATION OF ALKALINE PROTEASE PRODUCTION BY STREPTOMYCES AMBOFACIENS IN FREE AND IMMOBILIZED FORMNayera A.M. Abdelwahed, Enas N. Danial, Noura El-Ahmady El-Naggar, Asem A. MohamedOptimization of alkaline protease production by Streptomyces ambofaciens NRRL 2420 in free and immobilized form was investigated using submerged fermentation technique. The optimum conditions for maximum alkaline protease production 342 unit mL-1 were 30°C at pH 8.5 and incubation time 96 h in free cell cultures using starch 20 g L-1 as carbon source and yeast extract 5 g L-1 … …American Journal of Biochemistry and Biotechnology, Vol. 10, Issue 1 · 28 Dec 2013 · 8,125 accessesView PDF Cite

Research ArticleOpen accessIn the abstractBiosorption of Textile Dye Using Immobilized Bacterial (Pseudomonas aeruginosa) and Fungal (Phanerochate chrysosporium) CellsNatarajan Saravanan, Tiruvenkadam Kannadasan, Chiya Ahmed Basha, Veerasamy ManivasaganWastewater containing dyes presents a serious problem due to its high toxicity which leads to creating enormous environmental pollution and ecological hazards. Therefore the removal of the high stable dyes from the textile effluents is of major importance. The purpose of this study is to remove the reactive dye Procion Blue 2G from textile dye solution by biosorption process using … …American Journal of Environmental Sciences, Vol. 9, Issue 4 · 16 Sep 2013 · 7,350 accessesView PDF Cite

Research ArticleOpen accessIn the abstractPotential Applications of Chitosan Nanoparticles as Novel Support in Enzyme ImmobilizationHoda Jafarizadeh Malmiri, Mohammad Ali Ghaz Jahanian, Aydin BerenjianChitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 11,302 accessesView PDF Cite