5 results for “Cattle Oocytes”

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Research ArticleOpen accessPhrase in the titleEffect of two Different Permitting and Combination of Cryoprotectants on Cattle Oocytes Maturation Rate Following Brilliant Cresyl Blue ExposureMasindi Lottus Mphaphathi, Hester Adri O'Neill, Tshimangadzo Lucky NedambaleThe objectives of this study were to elucidate the toxicity of Dimethyl sulfoxide (DMSO), Ethylene glycol (EG), and the combination of both permitting cryoprotectants (CPAs) to cattle oocytes and the effectiveness of Brilliant cresyl blue (BCB) on immature oocytes pre-selection. Cattle ovaries were collected from the local slaughterhouse and transported within 2 hours of slaughter. The oocytes were exposed to … …American Journal of Animal and Veterinary Sciences, Vol. 17, Issue 2 · 6 May 2022 · 6,201 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleEffect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro FertilizationMaleke Dimpho Sebopela, Masindi Lottus Mphaphathi et al.The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 4 · 16 Nov 2023 · 5,319 accessesView PDF Cite

Research ArticleOpen accessPhrase in the titleComparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo ProductionSindisiwe Mbali Sithole, Masindi Lottus Mphaphathi et al.The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved … …American Journal of Animal and Veterinary Sciences, Vol. 18, Issue 1 · 23 Feb 2023 · 6,912 accessesView PDF Cite

Research ArticleOpen accessIn the abstractIsolation and Culture of Oocytes from Dairy Cows' Ovaries for Productivity in the Southeastern Region of KazakhstanDauren Maratovich Bekenov, Batyrkhan Azimkhanovich Buralkhiev, Yusupzhan Artykovich Yuldashbayev et al.This research work aims to study methods for isolating and culturing follicular oocytes from Holstein cows in vitro. The proven complex of technologies for embryo transplantation on an industrial scale will provide a 3-5-fold acceleration of reproduction of highly productive cows. As a result of scientific research, biotechnological methods of accelerated reproduction of dairy cattle have been substantiated and introduced … …OnLine Journal of Biological Sciences, Vol. 24, Issue 4 · 14 Oct 2024 · 4,298 accessesView PDF Cite

Research ArticleOpen accessIn the abstractEffects of Age and Season on in vivo Embryo Production of Friesian Holstein CowsNurul Isnaini, Syafira Aldina Febrianty, Faizal Andri et al.Embryo transfer is one of the leading technologies that is now being applied in an effort to increase the population and productivity of dairy cattle in Indonesia. Several factors may affect embryo production including age and season. In this study, the effects of age and season on in vivo embryo production were evaluated on Friesian Holstein (FH) cows. A total … …American Journal of Animal and Veterinary Sciences, Vol. 19, Issue 1 · 2 Mar 2024 · 5,457 accessesView PDF Cite