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11 results for “C-terminal domain”
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Research ArticleOpen accessIn the keywordsIdentification a Novel Raw-Starch-Degrading-α-Amylase from a Tropical Marine BacteriumZeily Nurachman, Alfredo Kono, Ocky Karna Radjasa, Dessy NataliaProblem statement: Bacteria from the surface of the tropical marine hard coral Acropora sp. were screened for producing raw-starch-degrading-α-amylase. Approach: Based on its 16s rDNA sequence, a bacterium that produced the highest amylolitic activity was identified as Bacillus amyloliquifaciens ABBD. The bacterial isolate secreted a α-amylase extracellularly and then the enzyme was partially purified by ammonium sulfate precipitation followed by … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,337 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Silico Sequence Analysis, Structure Prediction and Function Annotation of Melanocortin 1 Receptor Gene (MC1R) from the Guppy Poecilia reticulataAbdul Khader Sultan Mohideen, Mehboob Asrar Sheriff, Kareem AltaffThe common guppy, Poecilia reticulata is one of the most popular aquarium fish used as a model organism in toxicology, embryology and genetical studies. Vibrant colors in males are a secondary sexual character which attracts the females for mating. Pigmentation in guppy is found to be linked with melanin synthesis. Melanocortin genes are involved in the synthesis of melanin pigments. … …American Journal of Biochemistry and Biotechnology, Vol. 11, Issue 4 · 17 Nov 2015 · 8,615 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Silico Analysis of Cellulose Synthase Gene (NcCesA1) in Developing Xylem Tissues of Neolamarckia CadambaTiong Shing Yiing, Ho Wei Seng, Pang Shek Ling, Ismail JusohThis study reported the isolation and in silico characterization of full-length cellulose synthase (CesA) cDNA from Neolamarckia cadamba, an important tropical plantation tree species. CesA is a member of processive glycosyltransferases that involved in cellulose biosynthesis of plants. CesA acts as a central catalyst in the generation of plant cell wall biomass or cellulose. It also plays an important role … …Current Research in Bioinformatics, Vol. 3, Issue 2 · 13 Jan 2015 · 7,995 accessesView PDFCite
Research ArticleOpen accessIn the abstractHigh Incidence of Disorder-Promoting Amino Acids in the Amino Terminus of Mature Proteins in Bacillus subtilisGuangqiang Wang, Yining Dong, Haiqin Chen et al.Problem statement: Exported polypeptides are usually synthesized as preproteins which are composed with mature domains and the signal peptides. The foreign proteins are usually inefficiently secreted by the aid of signal peptides. Although we know that the mature protein played an important role in the protein export process, we do not know what properties of the mature domains are critical … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 4 · 2 Feb 2012 · 8,258 accessesView PDFCite
Research ArticleOpen accessIn the abstractElectrical and Magnetoresistive studies Nd doped on La-Ba-Mn-O3 Manganites for Low-field Sensor applicationH. Abdullah, S. A. HalimProblem Statement: Electrical and magnetoresistive properties of the Nd doped (La1-xNdx)0.5Ba0.5MnO3 type samples with 0 ≤ x ≤ 1.0 had been prepared using the solid state reaction. These materials are extensively studied by the substitution of rare-earth compound is to understand the nature of transport phenomena in each system. Approach: The samples were calcined at 900°C for 12 h, pelletized … …American Journal of Engineering and Applied Sciences, Vol. 2, Issue 2 · 30 Jun 2009 · 7,666 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Different Control Strategies in GA-Based Optimized UPFC Controller in Electric Power SystemsSeyed Abbas Taher, Reza Hematti, Majid NematiThis study presents a study of the application of a Unified Power Flow Controller (UPFC) to control power transmission, generator terminals voltage and better damping of Low Frequency Oscillation (LFO) in Single-Machine Infinite-Bus (SMIB) power system. The UPFC integrates properties of both shunt and series compensations and can effectively alter power system parameters in a way that increases power transfer … …American Journal of Engineering and Applied Sciences, Vol. 1, Issue 1 · 31 Mar 2008 · 7,606 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression of Thioredoxin-Fusion Proteins of α-Gliadin, γ-Gliadin and Low Molecular Weight Glutenin, from Wheat Endosperm and their Domains in EnterobacteriaClaudia G. Benitez-Cardoza, Yves Popineau, Jacques GueguenWheat seed storage proteins play a determining role in the viscoelastic properties of wheat gluten. The genes encoding α-gliadin, γ-gliadin and Low Molecular Weight glutenin and their N-central-repetitive and C-terminal domains from wheat endosperm had been subcloned into a thioredoxin expression system (pET102/D-Topo) and produced as fusion proteins in E. coli. The expression levels for each of the proteins varied … …American Journal of Infectious Diseases, Vol. 3, Issue 2 · 30 Jun 2007 · 6,748 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Characterization of Soybean Mosaic Virus NIa Protein and its Processing Event in Bacterial ExpressionBong K. Choi, Jae S. Park, Hye J. Ahn et al.Soybean mosaic virus (SMV)-CN18 is an Rsv resistance-breaking (RB) isolate to overcome soybean resistance genes Rsv1, Rsv3 and Rsv4. The aim of this study was to characterize nuclear inclusion protein a (NIa protein) of RB isolate at the molecular level and demonstrate its processing into genome-linked protein (VPg) and NIa-Pro domains in Esherichia coli containing a bacterial expression pET vector … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 4 · 31 Dec 2006 · 6,906 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Complementation Studies of Human Pin1 in Azotobacter vinelandii Revealed that it Requires Amino Terminus of the NifM to Deliver PPIase Effect to the Fe-protein of NitrogenaseKumaraguru Raja, Lakshmi Pulakat, Nara GaviniThe NifM is a peptidyl prolyl cis-trans isomerase and is required for the maturation and activation of the Fe protein of Nitrogenase. Since the carboxyl terminus of NifM is similar to the Human Pin1, we expressed the Human Pin1 in A. vinelandii BG98, a nifM mutant strain containing a kanamycin insertion and found that it could not complement the function … …American Journal of Biochemistry and Biotechnology, Vol. 2, Issue 1 · 31 Mar 2006 · 6,710 accessesView PDFCite
Research ArticleOpen accessIn the abstractHuman Latrophilin-2 is Expressed in the Cytotrophoblast and Syncytiotrophoblast of Placenta and in Endothelial CellsGunda Herberth, Anke Stein, Jens Glienke et al.Latrophilin-2 is a member of the family of adhesion-GPCRs that is characterised by a long N-terminus which contains motifs identified in proteins involved in cell adhesion. We were interested in determining the expression pattern of human latrophilin-2 and to perform a biochemical characterisation of this protein. The expression pattern of latrophilin-2 was analysed in human organs, tissues and cell lines. … …American Journal of Biochemistry and Biotechnology, Vol. 1, Issue 3 · 30 Sep 2005 · 8,058 accessesView PDFCite