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15 results for “Bovine serum albumin”
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Research ArticleOpen accessPhrase in the titleThe Effect of Trilobatin on Bovine Serum Albumin: Interaction Mechanism and Molecular DockingYuhan Zhai, Yuqing Zhang, Yaping Li et al.Understanding the transport and distribution of small molecules in vivo is aided by studying their interactions with proteins. As a natural sweetener, trilobatin possesses several biological activities. However, uncertainty surrounds the trilobatin and Bovine Serum Albumin (BSA) interaction mechanism. In this study, the binding types, number of binding sites and binding constants of trilobatin interaction with BSA were studied by … …American Journal of Biochemistry and Biotechnology, Vol. 20, Issue 1 · 29 Mar 2024 · 4,843 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleBinding Interaction between Bovine Serum Albumin and Chicoric Acid, a Food Functional ComponentHaifang Xiao, Quancai Sun, Xuebo Liu, Yuanda SongFuorescence, FTIR and UV-Vis absorption spectroscopy were used to explore the binding between chicoric acid and Bovine Serum Albumin (BSA). Binding characteristics at various levels of temperature have been calculated. The results indicated that chicoric acid statically quenched the intrinsic fluorescence of BSA. The binding constants (Ka) were 4.14×105 L mol-1 at 273K and 4.29×106 L mol-1 at 298 k. … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 3 · 19 Sep 2016 · 7,552 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleBinding Mechanism of Bovine Serum Albumin to Troxerutin by Synchronous Fluorescence Spectroscopy and ChemometricsTianhu Wang, Yi Yan, Yinsheng LuoPrevious works have reported the binding of Bovine Serum Albumin (BSA) to troxerutin by absorption and fluorescence spectroscopy. In order to extend the investigation of their binding characteristics, the study presented here was designed to evaluate their interaction by the synchronous fluorescence spectroscopy and Multivariate Curve Resolution With Alternating Least Squares (MCR-ALS) under simulative physiological conditions. The Evolving Factor Analysis … …American Journal of Biochemistry and Biotechnology, Vol. 12, Issue 2 · 14 May 2016 · 7,449 accessesView PDFCite
Research ArticleOpen accessPhrase in the titlePRODUCTION OF CROSS-REACTIVE AUTOANTIBODY BINDING TO BOVINE SERUM ALBUMIN IN THE D-GALACTOSE-INDUCED AGING MOUSE MODELJi-Hun Park, Tae-Saeng ChoiThe D-galactose (D-gal)-induced animal model, generated by repeated subcutaneous D-gal injections over approximately 6 weeks, has been frequently used for diabetes and aging research. However, little research has investigated the direct correlation between D-gal and autoantibody formation despite several reports on diabetes-and aging-related autoantibodies. The purpose of this study was to determine whether repetitive injection of D-gal can induce autoantibody … …American Journal of Immunology, Vol. 10, Issue 1 · 13 Jan 2014 · 7,039 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleStabilization of Tyrosinase-Bovine Serum Albumin Crystals by GlutaraldehydeD. Norouzian, A. Akbarzadeh, M.H. Abnosi et al.Tyrosinase and bovine serum albumin were co-crystallized by saturated ammonium sulfate solution(65%) and 20% polyethylene glycol ( PEG) 6000 and n-propanol as co-solvents .The obtained crystals were cross linked by glutaraldehyde solution(1% v/v).Polyethylene glycol 6000 was found to be better co-solvent than n-propanol. The developed biocatalyst could be recycled 6 times without further loss of tyrosinase activity. No loss of … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 30 Sep 2007 · 7,194 accessesView PDFCite
Research ArticleOpen accessPhrase in the titleRelation between Macroscopic Binding Constant and the Anticancer Efficacy of the Bovine Serum Albumin-Quercetin Complex against Drug-Sensitive and Drug-Resistant CellsWinit Choiprasert, Chatchanok Loetchutinat, Nathupakorn Dechsupa, Samlee MankhetkornProblem statement: We have previously analyzed the interaction of BSA with flavonoids by using FRET. In this study, the role of BSA on increasing in solubility and on carrying the quercetin derivatives thus enhanced their anticancer efficacy against drug-sensitive and drug-resistant cells were conducted. Approach: The macroscopic (KD) and microscopic (kd) binding constant of the complexation and the cellular partition … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 1 · 27 May 2011 · 8,292 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBinding of the scutellarin to albumin using tryptophan fluorescence quenching, CD and FT-IR spectraJianniao Tian, Jiaqin Liu, Zhide Hu, Xingguo ChenThe binding of scutellarin with bovine serum albumin (BSA) was investigated at three temperatures, 296k, 310K, and 318K, by the fluorescence, circular dichroism (CD) and fourier transform infrared spectroscopy (FT-IR) at pH 7.40. The binding parameters were determined by Stern-Volmer equation. The thermodynamic parameters were calculated according to the dependence of enthalpy change on the temperature as follows:ΔH0 and ΔS0 … …American Journal of Immunology, Vol. 1, Issue 1 · 31 Mar 2005 · 13,643 accessesView PDFCite
Research ArticleOpen accessIn the keywordsExtraction, Purification and Characterization of Fish Chymotrypsin: A ReviewLiang Zhou, Suzanne M. Budge, Abdel E. Ghaly et al.Problem statement: Solid fish waste is generated from the unwanted parts of fish including heads, tails, fins, frames, offal (guts, kidney and liver) and skin. It accounts for up to 80% of material from production of surimi, 66% from production of fillet and 27% from production of headed and gutted fish. Currently, fish wastes are disposed off in land-based waste … …American Journal of Biochemistry and Biotechnology, Vol. 7, Issue 3 · 22 Sep 2011 · 10,585 accessesView PDFCite
Research ArticleOpen accessIn the abstractInhibitory Effect of Crude Walnut Leaf Polysaccharides on Oxidative Damage of Proteins and Proliferation of SGC-7901 CellsShuqing Yang, Zhike Xie, Shuyan Yu et al.In this paper, we studied the inhibitory effect of crude Walnut Leaf Polysaccharides (WLP) on free radical-induced oxidative damage of proteins and anti-proliferation activity on human Gastric Cancer cells (SGC-7901). The oxidative degradation of Bovine Serum Albumin (BSA), mouse brain proteins (MBP) and Mouse Liver Proteins (MLP) induced by hydroxyl radical and alcoxyl radical was analyzed by Sodium Dodecyl Sulfate-Polyacrylamide … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 26 Apr 2020 · 7,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Platelet Rich Plasma on Post Cryopreservation Viability, Morphology and Proliferation of Human Umbilical Cord Stem CellsNoviyanti Goei, Isabella Kurnia Liem, Jeanne Adiwinata Pawitan, Dian MedianaIn most cryopreservation medium, Fetal Bovine Serum (FBS) is used as supplement, while it is well known that FBS contains xenoproteins that can be incorporated into the cells and may be harmfull, as they can elicit immune response. Therefore, finding other xenofree materials as FBS alternative in cryopreservation medium is very important. Platelet Rich Plasma (PRP) is albumin rich and … …OnLine Journal of Biological Sciences, Vol. 15, Issue 2 · 4 Jun 2015 · 7,386 accessesView PDFCite