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8 results for “Agrobacterium”
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Research ArticleOpen accessEvery word in the titleChlorophyll and Protein Changes Observed Post Co-Cultivation of Explants with Agrobacterium Tumefaciens in Soybean (Glycine max L. Merr.)Phetole MangenaAlthough, Agrobacterium-mediated genetic transformation represents a major milestone in agricultural biotechnology for crop improvement against biotic and abiotic stresses. Further insights on the interactions between this bacterium and explants required for in vitro plant regeneration remain scant. This study investigated the changes in chlorophyll content and protein profiles of cotyledonary node explants co-cultivated with A. tumefaciens in soybean. Soybean seeds … …American Journal of Biochemistry and Biotechnology, Vol. 18, Issue 4 · 7 Dec 2022 · 5,046 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleResponse of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache RugosaWoo Tae Park, Thanislas Bastin Baskar, Sun Kyung Yeo et al.In this study a total of seven different Agrobacterium rhizogenes strains were evaluated for their ability to transform the plant Agastache rugosa and to produce the secondary metabolite rosmarinic acid. All the strains of A. rhizogenes i.e., 13333, 15834, A4, LBA9402, R1000, R1200 and R1601 strains tested here in this study, were able to induce hairy root formation in leaf … …OnLine Journal of Biological Sciences, Vol. 17, Issue 3 · 3 Sep 2017 · 7,866 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleThe Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensisP. Arokiaraj, R. Leelawathy, H. Y. YeangProblem statement: The present study investigates the ability of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance genetic transformation in Hevea brasiliensis. Approach: Hevea anther callus was transformed via Agrobacterium-mediated genetic transformation using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens A281. The transformed callus … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 3 · 30 Sep 2009 · 8,920 accessesView PDFCite
Research ArticleOpen accessEvery word in the titleAgrobacterium-mediated Genetic Transformation in Cucumber (Cucumis sativus L.)A. Vasudevan, N. Selvaraj, A. Ganapathi, C.W. ChoiThe present study was undertaken to develop efficient transformation protocol for cucumber cv. Poinsett 76 using Agrobacterium strain EHA 105. Five-day-old mature cotyledon explants was used for transformation study. The infected explants were co-cultivated for 2 days in MS medium containing BA (1.0 mg Lˉ1). The selection of transformed shoots was carried out in MS medium fortified with BA (1.0 … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 1 · 31 Mar 2007 · 8,901 accessesView PDFCite
Research ArticleOpen accessIn the keywordsA NAPIN PROMOTER ACTIVATES GENE EXPRESSION IN DEVELOPING SEEDS OF LESQUERELLA FENDLERIGrace Q. Chen, Jiann-Tsyh LinLesquerella fendleri produces industrial useful Hydroxy Fatty Acids (HFA) in seed oil. To improve oil and HFA of L. fendleri, it is desirable to use of seed-specific promoters to control the expression of target genes by genetic engineering. A seed-specific promoter fragment, -397 to -1 of a napin gene (PnapA) from Brassic napus was isolated by PCR and constructed to … …OnLine Journal of Biological Sciences, Vol. 12, Issue 3 · 18 Sep 2012 · 7,890 accessesView PDFCite
Research ArticleOpen accessIn the keywordsTransformation of Lesquerella Fendleri with the New Binary Vector pGPro4-35SGrace Q. Chen, Roger Thilmony, Jiann-Tsyh LinProblem statement: Crop genetic engineering requires the use of various promoters to control the expression of introduced transgenes. Some of the binary vectors currently available for promoter characterization in dicotyledonous plants have pitfalls due to their construction, such as containing a selectable marker cassette with enhancer sequences that can potentially interfere with the expression specificity of nearby promoters. Also, many … …OnLine Journal of Biological Sciences, Vol. 11, Issue 3 · 19 Jan 2012 · 7,425 accessesView PDFCite
Research ArticleOpen accessIn the abstractDevelopment of Transgenic Maize using Immature Embryos of HiII Genotype as a Vaccine CandidateHyun A. Kim, Suk Yoon Kwon, Han Sang Yoo et al.Problem statement: Plant-based vaccines posses some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs and wider use of vaccines for veterinary medicine. These study was undertaken to develop the transgenic maize as edible vaccine candidate for animals. Approach: The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 1 · 5 Mar 2012 · 7,567 accessesView PDFCite
Research ArticleOpen accessIn the abstractTransgenic Indian Cotton (Gossypium hirsutum) Harboring Rice Chitinase Gene (Chi II) Confers Resistance to Two Fungal PathogensM. Ganesan, P. Bhanumathi, K. Ganesh Kumari et al.Problem statement: The present investigation described a simple and reproducible protocol for transgenic cotton regeneration and characterization of chitinase (Chi II) gene expression against two different fungal pathogens in cotton. Approach: Transgenic cotton (Gossypium hirsutum cv. SVPR2) plants were produced by pCambia-bar-Chi II (13.8 kb) under the control of the CaMV 35S promoter, harbored in the strain LBA 4404 Agrobacterium … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 8,531 accessesView PDFCite