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7 results for “α-galactosidase”
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Research ArticleOpen accessIn the keywordsChaenomeles Sinensis: A Potent α-and β-Glucosidase InhibitorShruti Sancheti, Sandesh Sancheti, Sung-Yum SeoProblem statement: Glycosidase inhibitors are vital sources for the treatment of diabetes type II with a special importance in pharmacology, food industry and biotechnology, since for diabetes control, different diets and drugs, especially herbal medicines are recommended in this era. Approach: While screening for the potent natural glycosidase inhibitors, we found the fruits of Chaenomeles sinensis (C. sinensis), as the … …American Journal of Pharmacology and Toxicology, Vol. 4, Issue 1 · 31 Mar 2009 · 12,071 accessesView PDFCite
Research ArticleOpen accessIn the abstractSeparation, Purification and Anti-aging Activity of Tobacco PolysaccharidesMengyue Huang, Jingwu Song, Shuaishuai Chang et al.To separate tobacco polysaccharides and investigate their antioxidant and anti-aging properties, crude tobacco polysaccharides were extracted from raw tobacco using hot water, followed by purification through DEAE-52 cellulose column chromatography. The molecular weight and monosaccharide composition of the isolated polysaccharides were analyzed using HPGPC and HPLC. The antioxidant activities of these tobacco polysaccharides were tested using DPPH, ABTS, hydroxyl radicals … …American Journal of Biochemistry and Biotechnology, Vol. 21, Issue 1 · 15 Jan 2025 · 5,103 accessesView PDFCite
Research ArticleOpen accessIn the abstractGenetic Analysis of Bifidobacteriaceae in a Fermented Milk and Colostrum Product in Relation to Immune FunctionMarco RuggieroThis study investigates the microbial composition of a commercially available Swiss fermented milk and colostrum product with a history of use in chronic condition management since 2014. The analysis, performed using the Axiom Microbiome Array, identified a high number of targets corresponding to microbial species classified as probiotics belonging to the Bifidobacteriaceae family. The array provided 28 unique targets, each … …American Journal of Immunology, Vol. 19, Issue 1 · 2 Aug 2024 · 9,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractPassage Effect on Aging of Human Umbilical Cord Derived Mesenchymal Stem CellDian Mediana, Isabella Kurnia Liem, Jeanne Adiwinata Pawitan, Noviyanti GoeiTo be used in regenerative medicine, cells should be checked for various conditions, including cell aging. This study aimed to learn senescent profile and its relation to cell viability, proliferation and cell size in various passages, which were done in α-MEM-10% PRP medium, until senescence-associated β-galactosidase (SA-β-Gal) positive cells were found. Stem cells were isolated from umbilical cord tissue by … …OnLine Journal of Biological Sciences, Vol. 15, Issue 3 · 31 Jul 2015 · 8,039 accessesView PDFCite
Research ArticleOpen accessIn the abstractBiotransformation Using Recombinant Cmp Sialic Acid Synthetase and α-2, 6-Sialyltran Sferase: Enzymatic Synthesis of SialosidesUlrike Hubl, Cynthia Sun, Shuguang Zhang et al.In this research, we successfully expressed recombinant CMP-sialic Acid Synthetase (CSS) from Neisseria meningitides and 2,6-Sialyltransferase (SAT) from Photobacterium damsela in E. coli BL21(DE3) fermented at a scale of up to 8 litres using individual plasmids pIRL-1 and pIRL-4b, respectively. After cell lysis with BugBuster, enzyme levels of 2U and 22U per litre were produced for CSS and SAT, respectively. … …American Journal of Biochemistry and Biotechnology, Vol. 8, Issue 4 · 25 Dec 2012 · 8,854 accessesView PDFCite
Research ArticleOpen accessIn the abstractAssessment of Site Specific Mutational Effect on Transcription Initiation at Escherichia coli PromoterS. Kannan, M. Krishnan, S. Sundarraj et al.Problem statement: It is widely accepted thought that the weak promoters control the RNA synthesis and play regulatory role in complex genetic networks in bacterial system. An experiment had been designed to address whether mutations in the -16/-17 region affect the rate of transcription at an activator-independent promoter in E. coli or not? Approach: The aim of this study was … …American Journal of Biochemistry and Biotechnology, Vol. 5, Issue 2 · 30 Jun 2009 · 7,555 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe NifX Protein is Involved in the Final Stages of FeMo-cofactor Transport to the MoFe ProteinSurobhi Lahiri, Byron Cole, Lakshmi Pulakat, Nara GaviniThe nitrogenase enzyme catalyzes the reduction of dinitrogen to ammonia and is composed of the Fe and MoFe proteins. The iron molybdenum cofactor (FeMo-co) of the MoFe protein is the site of active substrate reduction. The NifX protein has also been suggested to have a role in the FeMo-co synthesis, although its exact role is still open to investigation. We … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 2 · 30 Jun 2007 · 7,262 accessesView PDFCite