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Research ArticleOpen accessIn the keywordsValidation of SSR markers associated with Submergence Tolerance in Rice (Oryza sativa L.)Kirill V. Azarin, Alexander V. Usatov, Andrey V. Alabushev et al.The present work is devoted to the validation of SSR markers associated with submergence tolerance (Sub1A) QTLs in rice. It was shown that only microsatellite markers RM 7481 differentiates donor lines and recipient variety. Based upon the results of DNA analysis with the marker of the RM 7481 of the F2-plants, the 14 homozygotes by the locus Sub1A, 40 samples … …American Journal of Agricultural and Biological Sciences, Vol. 11, Issue 4 · 19 Dec 2016 · 7,640 accessesView PDFCite
Research ArticleOpen accessIn the keywordsSwarm Optimization Techniques for Segmenting Gel Electrophoresis ImagesSara Ibrahim Ibrahim, Mohamed Abd Allah Makhlouf, Ghada.S. El-Tawel, M.E. WahedGel Electrophoresis (GE) are discussed as the main tool to dissociate DNA sequences. It helps in analyzing the genome such that each image resulting from it consists of lanes that include several bands. Image segmentation plays the foremost role in image processing. It helps in producing accurate results in medical diagnosis. Image segmentation works by dividing an image into regions … …Current Research in Bioinformatics, Vol. 5, Issue 1 · 18 Jun 2016 · 8,098 accessesView PDFCite
Research ArticleOpen accessIn the keywordsOne-Sided Multivariate Tests for High Dimensional DataSamruam ChongcharoenProblem statement: For a multivariate normal population with size smaller than dimension, n<p, the likelihood ratio tests of the null hypothesis that the mean vector was zero with a one-sided alternative were no longer valid because they involved with sample covariance matrix which was singular. Approach: The test statistics for one-sided multivariate hypotheses with n<p were proposed. Results: The simulation … …Journal of Mathematics and Statistics, Vol. 8, Issue 2 · 20 Jun 2012 · 7,264 accessesView PDFCite
Research ArticleOpen accessIn the keywordsStructural and Evolutionary Analysis of PARPs in D. discoideumA. Mehadvi Kawal, Hina A. Mir, Chethan Kumar Ramniklal et al.Problem statement: Dictyostelium discoideum, a unicellular eukaryote, exhibits multicellularity upon nutrient starvation, making it a better model for developmental studies and for the study of various signal transduction pathways. The most felicitous point of interest is that many of its genes show high degree of similarity to vertebrate genes. Poly (ADP-ribose) polymerase (PARP), a ubiquitous and abundant nuclear protein, has … …American Journal of Infectious Diseases, Vol. 7, Issue 3 · 3 Nov 2011 · 7,855 accessesView PDFCite
Research ArticleOpen accessIn the keywordsInversion of Covariance Matrix for High Dimension DataSamruam ChongcharoenProblem statement: In the testing statistic problem for the mean vector of independent and identically distributed multivariate normal random vectors with unknown covariance matrix when the data has sample size less than the dimension n≤p, for example, the data came from DNA microarrays where a large number of gene expression levels are measured on relatively few subjects, the p×p sample … …Journal of Mathematics and Statistics, Vol. 7, Issue 3 · 27 Jul 2011 · 7,874 accessesView PDFCite
Research ArticleOpen accessIn the keywordsEarly Diagnosis of Breast Cancer using Molecular, Biochemical and Pathological MarkersSalah El-Din El-Assal, Adel A. El-Tarras, Samir M. Abd-allaProblem statement: Laboratory diagnosis of breast cancer in most of the hospitals has traditionally been performed using cell culture and the direct hormone receptor assay, which are money and time consuming. Approach: This study was performed in order to direct the attention toward increasing the efficiency of early diagnosis in clinical laboratories at the western region of KSA and Egypt … …American Journal of Applied Sciences, Vol. 8, Issue 1 · 31 Jan 2011 · 8,060 accessesView PDFCite
Research ArticleOpen accessIn the keywordsCloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-GluZeily Nurachman, Sari Dewi Kurniasih, Ferra Puspitawati et al.Problem statement: An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 9,727 accessesView PDFCite
Research ArticleOpen accessIn the keywordsIsolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular MethodAngela Ingianni, Marta Quartuccio, Maria Antonietta Madeddu et al.The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 … …American Journal of Infectious Diseases, Vol. 3, Issue 3 · 30 Sep 2007 · 7,256 accessesView PDFCite
Research ArticleOpen accessIn the keywordsBiomedicine and Informatics Model of Alzheimer's DiseaseKang Cheng, Chang-Hua ZouIn a perspective of biomedicine and informatics, the mechanism of Alzheimer's, senile amnesia, or other aging-associated and cognitive impairment related diseases involve four important informative processing procedures: propagation, consolidation, retrieval and cognition, In this study, we systematically model the four procedures based on published experimental data. When modeling the propagation, we develop an equivalent circuit of biological membrane to describe … …American Journal of Biochemistry and Biotechnology, Vol. 3, Issue 3 · 28 May 2007 · 6,828 accessesView PDFCite
Research ArticleOpen accessIn the keywordscDNA Microarray Genome Image Processing Using Fixed Spot PositionBasim Alhadidi, Hussam Nawwaf Fakhouri, Omar S. AlMousaComplementary DNA (cDNA) microarray is one of the most recent and important technology for exploring the genome. cDNA microarray Image analysis aims to measure the intensity for each spot in the scanned image and this intensity represents the amount of a specific gene in the studied cell. This paper presents a new algorithm that achieves an automated way for applying … …American Journal of Applied Sciences, Vol. 3, Issue 2 · 28 Feb 2006 · 7,297 accessesView PDFCite