cDNA Microarray Genome Image Processing Using Fixed Spot Position

Complementary DNA (cDNA) microarray is one of the most recent and important technology for exploring the genome. cDNA microarray Image analysis aims to measure the intensity for each spot in the scanned image and this intensity represents the amount of a specific gene in the studied cell. This paper presents a new algorithm that achieves an automated way for applying grid in the cDNA microarray images through the determination of spots position in the cDNA microarray and calculating the mean of every raw and column in the microarray image and the result will be a fast automated system for applying addressing (grid) on the microarray image without any human interaction and this automated system starts from the step that follow the sub microarray image cropping.