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94 results for “primer”
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Research ArticleOpen accessIn the abstractUtilization of Rumen Content by Indigenous Microorganisms in a Modified Anaerobic DigesterAlhassan Salihu Adam, Sani Yahaya, Haruna Musa et al.The current work was designed to assess the effect of modification of model digester on the kinetics that affects efficiency in biogas generation using bovine rumen content as the feedstock. A biogas plant consisting of conventional and modified fixed-dome digesters (each with 2 m3 capacity) was established; and bovine rumen content was used as feedstock. Standard methods were used to … …Energy Research Journal, Vol. 11, Issue 1 · 26 Sep 2020 · 6,780 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparative Effect of Ulvan and Biosynthesized Silver Nanoparticles on Different Cell Lines Cytotoxicity and Gene ExpressionHadear Hanie Amin, Mohammed Ahmed Taha AbdelreheemUntil now, researchers searching for compounds that contribute for treating the most serious disease of this age, cancer. Researchers hope to contribute to this through this research. Activities of both Ulvan extracted from Ulva lactuca and silver nanoparticles (Ag-NPs) were examined against seven different cancer cell types called (Caco2 cells, A549 cells, Mcf7 cells, Hep2 cells, HepG2 cells, PC3 cells, … …OnLine Journal of Biological Sciences, Vol. 20, Issue 2 · 30 Jun 2020 · 6,675 accessesView PDFCite
Research ArticleOpen accessIn the abstractDetection of Commercial Fraud in Processed Meat Products Using Rapid TechniquesAli M. Ahmed, Takwa H. Ismail, Reham R. Abouelmaatti et al.Meat adulteration with different species has become a considerable problem worldwide. It means that meat products contain undeclared meat species; so, the meat ingredients are not consistent with the label. It has direct impact on public health, religious faith, fair-trades and food safety standers. This study focused on species adulteration detection in meat products by applying accurate, slandered and fast … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 4 Jun 2020 · 7,817 accessesView PDFCite
Research ArticleOpen accessIn the abstractcDNA Cloning and Characterization of the House Dust Mite Allergen Sui p 2Joshua Evans M. Bajao, John Donnie A. RamosSuidasia pontifica (Sp) is a House Dust Mite (HDM) species found in tropical urban residential areas which causes sensitization of up to 75% of allergic patients. Cloning of allergens from Sp is a significant initial step in understanding the clinical significance of this HDM species. The study describes the cloning and in silico characterization of the group 2 allergen gene … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 2 · 14 May 2020 · 7,885 accessesView PDFCite
Research ArticleOpen accessIn the abstractDNA Yields and PCR Amplification Success Using Degraded Animal CorpsesJimmy Ilunga, Paul Michael, Grace Daniel et al.A recurring problem with ancient specimens from wildlife animals is that their preserved tissues contain small amounts of DNA in a degraded state. The specific objectives of the present study were: (1) To determine DNA yields from different animal tissues; (2) to compare traditional (manual) DNA extraction protocols with commercial procedures and (3) to assess the success of PCR amplification … …American Journal of Biochemistry and Biotechnology, Vol. 16, Issue 1 · 12 Feb 2020 · 7,479 accessesView PDFCite
Research ArticleOpen accessIn the abstractOne Real-Time Fluorescent Loop-Mediated Isothermal Amplification Combined with Propidium Monoazide for Detection of Viable Vibrio Parahaemolyticus in SeafoodHuanteng Yu, Jiehong Fang, Biao Ma et al.Vibrio parahaemolyticus is one of the crucial foodborne pathogens in the world. At present, the rapid detection methods for V. parahaemolyticus cannot distinguish between dead and viable cells and false positive results may occur. In this study, one rapid and accurate method that combines Propidium Monoazide (PMA) with real-time fluorescent loop-mediated isothermal ampli?cation (LAMP) using SYTO-16 dye was developed to … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 2 · 26 Jun 2019 · 7,589 accessesView PDFCite
Research ArticleOpen accessIn the abstractComparison of Molecular Markers ISSR, SSR and RFLP to Study the Genetic Variation of Strawberry CultivarsGanies Riza Aristya, Rina Kasiamdari, Muhammad Fauzi Arif et al.Strawberry is grown in Indonesia because it has potential high economic value. The purpose of this study was to investigate relationship of phenetic lineage and genetic variations in 12 strawberry cultivars using three different molecular marker methods, including ISSR, SSR and RFLP. Five primers of ISSR markers were used and they generated 53 bands with 44 polymorphic DNA bands. Percentage … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 19 Mar 2019 · 9,182 accessesView PDFCite
Research ArticleOpen accessIn the abstractIdentification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)Nastaran Moarefi, Paul Michael, Peter Beckett, Kabwe NkongoloBetula is a polyploid and highly polymorphic genus with several species known to hybridize very readily. This high level of introgression has resulted in hybrid populations where different species are sympatric. The main objectives of the present study were to identify putative hybrids between B. papyrifera and B. pumila in the Greater Sudbury Region (Northern Ontario) and to develop ISSR … …American Journal of Environmental Sciences, Vol. 14, Issue 5 · 30 Nov 2018 · 8,057 accessesView PDFCite
Research ArticleOpen accessIn the abstractSensitive and Rapid Detection of Sulfate-Reducing Bacteria in Jet Fuel by Loop-Mediated Isothermal Amplification Combined with Lateral Flow DipstickZezhen Li, Yun Xiong, Peng Zhu, Hailong HuangThe existence of Sulfate-Reducing Bacteria (SRB) is one of the significant reasons for the Microbially Influenced Corrosion (MIC) of jet fuel. Especially for the jet fuel stored by military, since jet fuel is stored in the tank for a long time, some oxygen-consuming bacteria such as Amorphotheca resinae and Bacillus Cohn can consume oxygen and generate organic acids at the … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 2 · 11 Apr 2018 · 10,395 accessesView PDFCite
Research ArticleOpen accessIn the abstractMolecular Analysis of KRAS Mutation Associated with Colorectal Cancer in Iraqi PatientsRehab Subhi Ramadhan, Aola Sabah Ali, Rebah Najah AlgafariThe role of KRAS gene was investigated in manifestation of colorectal cancer in Iraqi patients. A total 40 blood samples were collected during October 2016 to January 2017 from AL-Amal Hospital in Baghdad and 20 blood samples from healthy subjects served as control. Blood samples were collected from subjects of 40-70 years old for both patients and control. The study … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 1 · 3 Mar 2018 · 9,024 accessesView PDFCite