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113 articles for “Protein Expression”
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Research ArticleOpen accessIn the abstractComparative Functional Analysis of the Basic Helix-Loop-Helix Proteins in the Clawed Frogs' Genomes With Common Essential Pathways and Enriched Gene Ontology TermsWuyi LiuThe international Gene Ontology (GO) and pathway databases were used to functionally analyze the clawed frogs' Basic Helix-Loop-Helix (bHLH) transcription factors of Xenopus tropicalis and Xenopus laevis in a updated genome-wide survey. There were 41 GO terms and one pathway significantly enriched for Xenopus tropicalis, whereas there were 45 GO terms and 3 pathways significantly enriched for Xenopus laevis. Among … …American Journal of Biochemistry and Biotechnology, Vol. 15, Issue 1 · 28 Mar 2019 · 8,497 accessesView PDFCite
Research ArticleOpen accessIn the abstractExposure to Mercury at Trace Concentrations Leads to Collapse of the Hepato-Nephrocitic System in Two Neotropical Species of BumblebeeFelipe Lissoni de Andrade Nogueira, Paulo José Bálsamo, Monica Jones Costa, Fábio Camargo AbdallaThis research aimed to investigate the effects of exposure to safe concentrations of mercury on the morpho-physiology of cells of the hepato-nephrocitic system of two species of neotropical bees. Workers of the neotropical bumblebee species Bombus morio and B. atratus were exposed to 0.2 ppb mercury for 48 h by ingestion. Bioassays were performed according some direction of OECD (2017) … …American Journal of Agricultural and Biological Sciences, Vol. 14, Issue 1 · 14 Dec 2018 · 8,620 accessesView PDFCite
Research ArticleOpen accessIn the abstractEfficient Production of Glycosylated and Non-Glycosylated Mycobacterium tuberculosis Antigen 85B Fused to PVX Coat Protein in Nicotiana benthamiana PlantsPablo A. González, Franco D. Puccio, Alicia M. ZeladaThe development of alternative subunit based-vaccines against tuberculosis is necessary due to variable efficiency and some security concerns of the BCG vaccine. The aim of this work was evaluate the production of the Mycobacterium tuberculosis Ag85B antigen fused to Potato Virus X Coat Protein (PVX-CP) by transient expression in Nicotiana benthamiana for subunit-based tuberculosis vaccine formulation. A codon-optimized M. tuberculosis … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 4 · 13 Nov 2018 · 9,980 accessesView PDFCite
Research ArticleOpen accessIn the abstractProduction of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coliDessy Natalia, Christian Heryakusuma, Fernita Puspasari et al.Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated … …American Journal of Biochemistry and Biotechnology, Vol. 14, Issue 3 · 10 Oct 2018 · 10,348 accessesView PDFCite
Research ArticleOpen accessIn the abstractSequence Analysis of Scaffold/Matrix Attachment Regions (S/MARs) From Human Embryonic Kidney and Chinese Hamster Ovary CellsNur Shazwani Mohd Pilus, Azrin Ahmad, Nurul Yuziana Mohd YusofBinding of intergenic Scaffold/Matrix Attachment Regions (S/MARs) to nuclear matrix proteins is believed to poise adjacent genes for transcription by forming chromatin loops. Vector constructs containing Scaffold/Matrix Attachment Regions (S/MAR) flanking the gene of interest, therefore, are able to enhance recombinant protein expression in mammalian cells. We compared two methods that are based on buffers containing 2M NaCl and Lithium-3,5-diidosalicylate … …OnLine Journal of Biological Sciences, Vol. 18, Issue 4 · 8 Oct 2018 · 7,637 accessesView PDFCite
Research ArticleOpen accessIn the abstractOligoasthenoteratozoospermia and Necrozoospermia: A Study of Sperm Na+, K+-ATPase α4 and Plasma Membrane Ca2+-ATPase 4 RegulationSilvia W. Lestari, Dessy Noor Miati, AsmarinahThe oligoasthenoteratozoospermia (OAT) and necrozoospermia are the extreme sperm abnormalities in male infertility. These sperm abnormalities may be related to the imbalance of ion transport which mediated by the ion-transporting P-type ATPases, such as Na+, K+-ATPase and Ca2+-ATPase. Until now, there is limited data about the activity of Na+, K+-ATPase and Ca2+-ATPase in sperms in the OAT and necrozoospermia samples. … …OnLine Journal of Biological Sciences, Vol. 18, Issue 3 · 3 Aug 2018 · 11,273 accessesView PDFCite
Research ArticleOpen accessIn the abstractTranscriptomic Analysis of the Erythritol High-Yielding Mutant Strain Yarrowia lipolytica Y44Xiaojie Ren, Heng Ban, Chao Huang et al.RNA-seq technique was used to analyze the transcriptomics of Yarrowia lipolytica control strain Po1g, an erythritol-producing natural isolate Yarrowia lipolytica Y 22 and an erythritol high-yielding mutant strain Y44 derived from Y 22. Functional annotation of genes and classification of metabolic pathways were carried out to identify Differentially Expressed Genes (DEGs). These DEGs were classified into related metabolic pathways to … …American Journal of Biochemistry and Biotechnology, Vol. 17, Issue 4 · 20 Dec 2021 · 6,119 accessesView PDFCite
EditorialOpen accessIn the abstractFocalize the Structure of Myd88 in TLR Signaling Pathway to Modulate Innate Immune Response: New Target for Old DiseasesShuai Xing, Ping ZhouAll diseases have their Achilles’ Heel to target and this is especially true for immunorelated diseases which must be treated by targeting the immune system somewhere and somehow correctly. In the most recent decade, immunologists recognized that the Toll-Like Receptor (TLR) signaling pathway in innate immune system plays a double-edged role in the pathogenesis of many notorious diseases. Although the … …American Journal of Immunology, Vol. 13, Issue 3 · 6 Sep 2017 · 7,865 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Pre-Slaughter Autophagic Status on Postmortem Proteolysis in Skeletal Muscle of MiceTomonori Nakanishi, Miho Yamashita, Naoto Nishimoto et al.Post mortem proteolysis is a key event during the aging process, where muscle converts to meat. However, its precise mechanism remains unclear. In this study, the effects of pre-slaughter autophagic status on post mortem proteolysis were investigated using mouse skeletal muscle. Mice were fasted for 14 h and administered with or without the autophagy inhibitor wortmannin, whereas control mice were … …American Journal of Animal and Veterinary Sciences, Vol. 12, Issue 2 · 10 Jul 2017 · 7,993 accessesView PDFCite
EditorialOpen accessIn the abstractIs Rerum® the New Coley's Vaccine?Marco RuggieroColey's Vaccine and Cancer Immunotherapy Dr. William B. Coley is credited for having been the first to translate the observation of an association between bacterial infections, high fever and cancer remission into a successful cancer treatment. However, it is worth noting that such an association dates back to at least one century before Coley's times; as Wiemann and Starnes from … …American Journal of Immunology, Vol. 13, Issue 2 · 31 May 2017 · 12,285 accessesView PDFCite