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52 results for “Chromatography-Mass”
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Research ArticleOpen accessIn the abstractSpeciation of Arsenic Across Water-Sediment Interface of Falgu RiverRajeeva Ranjan, Rekha Rani, Ashay Bavishi et al.Two predominant species of arsenic compounds, As(III) and As(V), are found in soils and natural water and they have been classified as carcinogens. The focus of the present study was to examine the speciation of Arsenic (As) across the water-sediment interface at the confluence of drainage for Gaya city and the Falgu River. Gas Chromatography (GC) coupled to Inductively Coupled … …American Journal of Environmental Sciences, Vol. 8, Issue 6 · 31 Dec 2012 · 6,873 accessesView PDFCite
Research ArticleOpen accessIn the abstractGC-MS Analysis and PLS-DA Validation of the Trimethyl Silyl-Derivatization TechniquesKamalrul Azlan Azizan, Syarul Nataqain Baharum, Habtom W. Ressom, Normah Mohd. NoorProblem statement: Comparison and validation analysis of the conventional and power-adjusted Microwave-Assisted (MA) techniques of TMS derivatization using Gas Chromatography-Mass Spectrometry (GC-MS) and Multivariate Analysis (MVA) of Partial Least Squares Analysis (PLS-DA). Further improvement of the conventional technique using vigorous shaking was tested and analyzed. Approach: Cross-validation and response permutation test of PLS-DA and S-plot of Orthogonal Partial Least Squares … …American Journal of Applied Sciences, Vol. 9, Issue 7 · 4 Jun 2012 · 10,320 accessesView PDFCite
Research ArticleOpen accessIn the abstractAromatic Compound Glucopyranoside from New Species Artocarpus thailandicusWanaree Boonkla, Siripit Pitchuanchom, Puttinan Meepowpan et al.Problem statement: Many Artocarpus plants belonging to the family Moraceae are found in Thailand and have been used as Thai traditional medicines. These plants are known to produce a variety of phenolic glycoside. A. thailandicus is the new species and this prompted us to make further investigations on the occurrence of these types of compounds in the new Artocarpus species. … …American Journal of Applied Sciences, Vol. 8, Issue 11 · 23 Sep 2011 · 8,016 accessesView PDFCite
Research ArticleOpen accessIn the abstractEffect of Plant Growth Regulators on Callus, Cell Suspension and Cell Line Selection for Flavonoid Production from Pegaga (centella asiatica L. urban)Suat Hian Tan, Radzali Musa, Arbakariya Ariff, Mahmood MaziahProblem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 11,254 accessesView PDFCite
Research ArticleOpen accessIn the abstractExtraction and Purification of Collagenase Enzymes: A Critical ReviewSaid M Daboor, Suzanne M Budge, Abdel E Ghaly et al.Problem statement: Enzymes have vital roles in several industrial processes (foods, cosmetics, nutraceuticals and pharmaceuticals) due to their highly selective nature and high activity at very low concentrations. Recent efforts to identify new sources of useful enzymes have been concentrated on the marine environment because of the potential to make use of processing wastes. About 35-50% of the mass of … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 4 · 31 Dec 2010 · 14,311 accessesView PDFCite
Research ArticleOpen accessIn the abstractExpression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis)Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing WangProblem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT) and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+) as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia … …American Journal of Biochemistry and Biotechnology, Vol. 6, Issue 3 · 30 Sep 2010 · 7,770 accessesView PDFCite
Research ArticleOpen accessIn the abstractIn Vitro Metabolism Studies of Polybrominated Diphenyl Ethers Using Rat and Human Liver MicrosomesShun Wen Cheng, Kathleen Randall, Anne T. KotchevarA number of studies have recently reported the bioaccumulation of the commonly used fire retardants, Polybrominated Diphenyl Ethers (PBDEs), in humans and wildlife. Exposure of animals to PBDEs has been shown to result in developmental neurological, reproductive abnormalities and the disruption of endocrine function. Thyroid hormone equilibria was also shown to be altered by PBDE exposure. There is evidence that … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 3 · 30 Sep 2008 · 6,998 accessesView PDFCite
Research ArticleOpen accessIn the abstractAltered Sulfur Amino Acid Metabolism In Immune Cells of Children Diagnosed With AutismJung H. Suh, William J. Walsh, Woody R. McGinnis et al.Autism Spectrum Disorder (ASD) is a behaviorally defined neurodevelopmental disorder whose etiology is poorly understood. Recent studies have shown that autistic children may be experiencing increased inflammation and oxidative stress. Altered immune regulation may be one contributing factor to inflammation and oxidative stress in autistic children. Sulfur amino acid (SAA) metabolism plays a critical role in regulating blood leukocyte functions … …American Journal of Biochemistry and Biotechnology, Vol. 4, Issue 2 · 30 Jun 2008 · 10,789 accessesView PDFCite
Research ArticleOpen accessIn the abstractMass Spectrometry/Mass Spectrometry Study on the Degradation of B-Aflatoxins in Maize with Aqueous Citric AcidA. Méndez-Albores, I. Nicolás-Vázquez, R. Miranda-Ruvalcaba, E. Moreno-MartÍnezDegradation of B-aflatoxins in maize by means of 1N aqueous citric acid was confirmed by the AFLATEST immunoaffinity column method, High Performance Liquid Chromatography (HPLC), tandem mass spectrometry (MS/MS) and computational information. The AFLATEST and HPLC assays showed that 96.7% degradation occurred in maize contaminated with 93 ng g-1 when treated with the aqueous citric acid. Two major products, produced … …American Journal of Agricultural and Biological Sciences, Vol. 3, Issue 2 · 30 Jun 2008 · 8,049 accessesView PDFCite
Research ArticleOpen accessIn the abstractThe Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma, Cheng Hock ChuahThis study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using … …American Journal of Applied Sciences, Vol. 4, Issue 6 · 30 Jun 2007 · 8,362 accessesView PDFCite